One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coli

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Caldinelli, Laura ^a,b   Albani, Diego ^c   Pollegioni, Loredano ^a,b  

^a UNIVERSITY OF INSUBRIA   (Italy)

^b POLITECNICO DI MILANO   (Italy)

^c MARIO NEGRI INSTITUTE FOR PHARMACOLOGICAL RESEARCH   (Italy)

Author keywords

Synuclein; Oxidative stress; Parkinson's disease; Protein aggregation; Recombinant proteins; TAT fusion protein

Indexed keywords

EXPERIMENTAL EVIDENCE; HIS-TAGGED PROTEINS; MICROMOLAR CONCENTRATION; PARKINSON'S DISEASE; PROTEIN AGGREGATION; PURIFICATION PROCEDURES; PURIFICATION PROTOCOL; SYNUCLEIN;

BACTERIOPHAGES; ESCHERICHIA COLI; METAL RECOVERY; NEURODEGENERATIVE DISEASES; OXIDATIVE STRESS; PURIFICATION;

RECOMBINANT PROTEINS;

ALPHA SYNUCLEIN; CHIMERIC PROTEIN; COMPLEMENTARY DNA; HYBRID PROTEIN; PERIPLASMIC PROTEIN; RECOMBINANT PROTEIN; TRANSACTIVATOR PROTEIN; TRANSACTIVATOR PROTEIN ALPHA SYNUCLEIN; UNCLASSIFIED DRUG;

ARTICLE; CHELATION; CHROMATOGRAPHY; CONTROLLED STUDY; CYTOPLASM; CYTOTOXICITY; DNA SYNTHESIS; ESCHERICHIA COLI; FIBER; GENE OVEREXPRESSION; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; NEUROPROTECTION; NONVIRAL GENE DELIVERY SYSTEM; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PARKINSON DISEASE; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

ALPHA-SYNUCLEIN; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; MICROSCOPY, CONFOCAL; PERIPLASM; RECOMBINANT FUSION PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; TAT GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS;

ESCHERICHIA COLI;

EID: 84875687780     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-32     Document Type: Article

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