A dual compartment diffusion chamber for studying axonal chemotaxis in 3D collagen

Journal of Neuroscience Methods

Volumn 215, Issue 1, 2013, Pages 53-59

  Pujic, Zac ^a   Goodhill, Geoffrey J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Axon guidance; Chemotropism; Gradient; NGF; Slit2

Indexed keywords

COLLAGEN; NERVE GROWTH FACTOR; SLIT2 PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ASSAY; CHEMOTAXIS; CONTROLLED STUDY; DIFFUSION CHAMBER; DORSAL ROOT; NERVE FIBER; NERVE FIBER GROWTH; NEURITE; NONHUMAN; OLFACTORY BULB; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; SPINAL GANGLION;

ANIMALS; AXONS; CHEMOTAXIS; COLLAGEN; DIFFUSION CHAMBERS, CULTURE; GANGLIA, SPINAL; IMMUNOHISTOCHEMISTRY; NERVE GROWTH FACTOR; NEURITES; OLFACTORY BULB; ORGAN CULTURE TECHNIQUES; RATS; RATS, WISTAR;

EID: 84875510147     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2013.02.008     Document Type: Article

References (32)

1. Balgude A.P., Yu X., Szymanski A., Bellamkonda R.V. Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures. Biomaterials 2001, 22:1077-1084.
2. Campenot R.B. Local control of neurite development by nerve growth factor. Proc Natl Acad Sci USA 1977, 74:4516-4519.
3. Cimetta E., Cannizzaro C., James R., Biechele T., Moon R.T., Elvassore N., et al. Microfluidic device generating stable concentration gradients for long term cell culture: application to Wnt3a regulation of beta-catenin signaling. Lab Chip 2010, 10:3277-3283.
4. Dudanova I., Gatto G., Klein R. GDNF acts as a chemoattractant to support ephrinA-induced repulsion of limb motor axons. Curr Biol 2010, 20:2150-2156.
5. Dudanova I., Kao T.J., Herrmann J.E., Zheng B., Kania A., Klein R. Genetic evidence for a contribution of EphA:ephrinA reverse signaling to motor axon guidance. J Neurosci 2012, 32:5209-5215.
6. Gallo G., Lefcort F.B., Letourneau P.C. The trkA receptor mediates growth cone turning toward a localized source of nerve growth factor. J Neurosci 1997, 17:5445-5454.
7. Gil V., del Rio J.A. Analysis of axonal growth and cell migration in 3D hydrogel cultures of embryonic mouse CNS tissue. Nat Protoc 2012, 7:268-280.
8. Goodhill G.J. Mathematical guidance for axons. Trends Neurosci 1998, 21:226-231.
9. Gundersen R.W., Barrett J.N. Neuronal chemotaxis: chick dorsal-root axons turn toward high concentrations of nerve growth factor. Science 1979, 206:1079-1080.
10. Kent C.B., Shimada T., Ferraro G.B., Ritter B., Yam P.T., McPherson P.S., et al. 14-3-3 proteins regulate protein kinase a activity to modulate growth cone turning responses. J Neurosci 2010, 30:14059-14067.
11. Kothapalli C.R., van Veen E., de Valence S., Chung S., Zervantonakis I.K., Gertler F.B., et al. A high-throughput microfluidic assay to study neurite response to growth factor gradients. Lab Chip 2011, 11:497-507.
12. Li H.S., Chen J.H., Wu W., Fagaly T., Zhou L., Yuan W., et al. Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons. Cell 1999, 96:807-818.
13. Liu W.W., Goodhouse J., Jeon N.L., Enquist L.W. A microfluidic chamber for analysis of neuron-to-cell spread and axonal transport of an alpha-herpesvirus. PLoS ONE 2008, 3:e2382.
14. Lohof A.M., Quillan M., Dan Y., Poo M.M. Asymmetric modulation of cytosolic cAMP activity induces growth cone turning. J Neurosci 1992, 12:1253-1261.
15. Lumsden A.G., Davies A.M. Earliest sensory nerve fibres are guided to peripheral targets by attractants other than nerve growth factor. Nature 1983, 306:786-788.
16. Mortimer D., Feldner J., Vaughan T., Vetter I., Pujic Z., Rosoff W.J., et al. Bayesian model predicts the response of axons to molecular gradients. Proc Natl Acad Sci USA 2009, 106:10296-10301.
17. Mortimer D., Fothergill T., Pujic Z., Richards L.J., Goodhill G.J. Growth cone chemotaxis. Trends Neurosci 2008, 31:90-98.
18. Mortimer D., Pujic Z., Vaughan T., Thompson A.W., Feldner J., Vetter I., et al. Axon guidance by growth-rate modulation. Proc Natl Acad Sci USA 2010, 107:5202-5207.
19. O'Donnell M., Chance R.K., Bashaw G.J. Axon growth and guidance: receptor regulation and signal transduction. Annu Rev Neurosci 2009, 32:383-412.
20. Pujic Z., Giacomantonio C.E., Unni D., Rosoff W.J., Goodhill G.J. Analysis of the growth cone turning assay for studying axon guidance. J Neurosci Methods 2008, 170:220-228.
21. Pujic Z., Mortimer D., Feldner J., Goodhill G.J. Assays for eukaryotic cell chemotaxis. Comb Chem High Throughput Screen 2009, 12:580-588.
22. Rosoff W.J., McAllister R., Esrick M.A., Goodhill G.J., Urbach J.S. Generating controlled molecular gradients in 3D gels. Biotechnol Bioeng 2005, 91:754-759.
23. Rosoff W.J., Urbach J.S., Esrick M.A., McAllister R.G., Richards L.J., Goodhill G.J. A new chemotaxis assay shows the extreme sensitivity of axons to molecular gradients. Nat Neurosci 2004, 7:678-682.
24. Ruiz de Almodovar C., Fabre P.J., Knevels E., Coulon C., Segura I., Haddick P.C., et al. VEGF mediates commissural axon chemoattraction through its receptor Flk1. Neuron 2011, 70:966-978.
25. Song H.J., Ming G.L., Poo M.M. cAMP-induced switching in turning direction of nerve growth cones. Nature 1997, 388:275-279.
26. Tessier-Lavigne M., Placzek M., Lumsden A.G., Dodd J., Jessell T.M. Chemotropic guidance of developing axons in the mammalian central nervous system. Nature 1988, 336:775-778.
27. Thompson A.W., Pujic Z., Richards L.J., Goodhill G.J. Cyclic nucleotide-dependent switching of mammalian axon guidance depends on gradient steepness. Mol Cell Neurosci 2011, 47:45-52.
28. Wang J.C., Li X., Lin B., Shim S., Ming G.L., Levchenko A. A microfluidics-based turning assay reveals complex growth cone responses to integrated gradients of substrate-bound ECM molecules and diffusible guidance cues. Lab Chip 2008, 8:227-237.
29. Xu J., Rosoff W.J., Urbach J.S., Goodhill G.J. Adaptation is not required to explain the long-term response of axons to molecular gradients. Development 2005, 132:4545-4552.
30. Yam P.T., Langlois S.D., Morin S., Charron F. Sonic hedgehog guides axons through a noncanonical, Src-family-kinase-dependent signaling pathway. Neuron 2009, 62:349-362.
31. Zicha D., Dunn G.A., Brown A.F. A new direct-viewing chemotaxis chamber. J Cell Sci 1991, 99(Pt 4):769-775.
32. Zigmond S.H. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J Cell Biol 1977, 75:606-616.