NP-40 reduces contamination by endogenous biotinylated carboxylases during purification of biotin tagged nuclear proteins

Protein Expression and Purification

Volumn 89, Issue 1, 2013, Pages 80-83

  Papageorgiou, Dimitris N ^a,b   Demmers, Jeroen ^c   Strouboulis, John ^a  

^a BIOMEDICAL SCIENCES RESEARCH CENTER ALEXANDER FLEMING   (Greece)

^b UNIVERSITY OF ATHENS   (Greece)

^c ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Nuclear extracts Affinity purification Protein complexes Transcription factors BirA biotin ligase Biotin tagging

Indexed keywords

BIOTIN; BIRA PROTEIN, E COLI; CELL EXTRACT; ESCHERICHIA COLI PROTEIN; LIGASE; MACROGOL DERIVATIVE; NONIDET P-40; NUCLEAR PROTEIN; REPRESSOR PROTEIN; TRITON X 114;

ARTICLE; BIOTINYLATION; CELL NUCLEUS; CHEMISTRY; ISOLATION AND PURIFICATION;

BIOTIN; BIOTINYLATION; CARBON-NITROGEN LIGASES; CELL EXTRACTS; CELL NUCLEUS; ESCHERICHIA COLI PROTEINS; NUCLEAR PROTEINS; POLYETHYLENE GLYCOLS; REPRESSOR PROTEINS;

EID: 84875477796     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.02.015     Document Type: Article

References (11)

1. M. Shoaib, A. Kulyyassov, C. Robin, K. Winczura, P. Tarlykov, E. Despas, P. Kannouche, E. Ramanculov, M. Lipinski, V. Ogryzko, PUB-NChIP - "in vivo biotinylation" approach to study chromatin in proximity to a protein of interest, Genome Res.
2. G. Strubbe, C. Popp, A. Schmidt, A. Pauli, L. Ringrose, C. Beisel, and R. Paro Polycomb purification by in vivo biotinylation tagging reveals cohesin and Trithorax group proteins as interaction partners Proc. Nat. Acad. Sci. USA 108 2011 5572 5577
3. V. van den Boom, M. Rozenveld-Geugien, F. Bonardi, D. Malanga, D. van Gosliga, A.M. Heijink, G. Viglietto, G. Morrone, F. Fusetti, E. Vellenga, J.J. Schuringa, Non-redundant and locus-specific gene repression functions of PRC1 paralog family members in human hematopoietic stem/progenitor cells, Blood (2013) (Epub ahead of print).
4. A. Chapman-Smith, and J.E. Cronan Jr. In vivo enzymatic protein biotinylation Biomol. Eng. 16 1999 119 125 (Pubitemid 30184848)
5. M.G. Cull, and P.J. Schatz Biotinylation of proteins in vivo and in vitro using small peptide tags Methods Enzymol. 326 2000 430 440
6. P. Rodriguez, H. Braun, K.E. Kolodziej, E. de Boer, J. Campbell, E. Bonte, F. Grosveld, S. Philipsen, and J. Strouboulis Isolation of transcription factor complexes by in vivo biotinylation tagging direct binding to streptavidin beads Meth. Mol. Biol. 338 2006 305 323
7. E. de Boer, P. Rodriguez, E. Bonte, J. Krijgsveld, E. Katsantoni, A. Heck, F. Grosveld, and J. Strouboulis Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice Proc. Nat. Acad. Sci. USA 100 2003 7480 7485 (Pubitemid 36759995)
8. S.M. Abmayr, T. Yao, T. Parmely, J.L. Workman, Preparation of nuclear and cytoplasmic extracts from mammalian cells, in: Frederick M. Ausubel et al. (Eds.), Current Protocols in Molecular Biology, Wiley Online Library, 2006 (Chapter 12, Unit 12 11).
9. J.D. Dignam, R.M. Lebovitz, and R.G. Roeder Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei Nucleic Acids Res. 11 1983 1475 1489
10. A. Shevchenko, M. Wilm, O. Vorm, and M. Mann Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels Anal. Chem. 68 1996 850 858
11. P. Rodriguez, E. Bonte, J. Krijgsveld, K.E. Kolodziej, B. Guyot, A.J. Heck, P. Vyas, E. de Boer, F. Grosveld, and J. Strouboulis GATA-1 forms distinct activating and repressive complexes in erythroid cells EMBO J. 24 2005 2354 2366 (Pubitemid 41032587)