The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients;Uso da Reação em Cadeia da Polimerase aninhada (PCR aninhada) para o diagnóstico precoce da infecção pelo Histoplasma capsulatum no soro e no sangue total de pacientes HIV positivos

Anais Brasileiros de Dermatologia

Volumn 88, Issue 1, 2013, Pages 141-143

  Dantas, Katia Cristina ^a   Freitas, Roseli S ^a   Vicentini Moreira, Adriana Pardini ^b   da Silva, Marcos Vinicius ^a   Vasconcellos, Cidia ^a   Benard, Gil ^a   Criado, Paulo Ricardo ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

AIDS related opportunistic infections; Histoplasma; HIV seropositivity; Molecular diagnostic techniques; Polymerase chain reaction

Indexed keywords

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ANTIGEN DETECTION; ARTICLE; DNA SEQUENCE; HISTOPLASMA CAPSULATUM; HISTOPLASMOSIS; HUMAN; IMMUNOBLOTTING; IMMUNODIFFUSION; IMMUNOREACTIVITY; INCIDENCE; MICROSCOPY; PNEUMONIA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROLOGY;

EARLY DIAGNOSIS; HISTOPLASMA; HISTOPLASMOSIS; HIV SEROPOSITIVITY; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84875407585     PISSN: 03650596     EISSN: 18064841     Source Type: Journal    
DOI: 10.1590/S0365-05962013000100025     Document Type: Article

References (7)

1. Wheat J, Hafner R, Wulfson M, Spencer P Squires K, Powderly W, et al. Prevention of relapse of histoplasmosis with itraconazole in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1993;118:610-6.
2. Maubon D, Simon S, Aznar C. Histoplasmosis diagnosis using a polymerase chain reaction method. Application on human samples in French Guiana, South America. Diagn Microbiol Infect Dis. 2007;58:441-4.
3. Kauffman CA. Histoplasmosis: a clinical and laboratory update. Clin Microbiol Rev. 2007;20:115-32.
4. Wheat LJ, Garringer, T, Brizendine E, Connolly FI Diagnosis of histoplasmosis by antigen detection based upon experience at the histoplasmosis reference laboratory. Diagn. Microbiol infect Dis. 2002;43:29-37.
5. Freitas RS, Carvalho-Vivi JO, Zamboni IM, Assis CM, Costa-Martins JE, Vicentini-Moreira AP. The importance of serological assays in diagnosing acute pulmonary histoplasmosis. J Venom Anim Toxins Inct Trop Dis. 2009;15:278-88.
6. Bialek R, Feucht A, Aepinus C, Just-Nubling G, Robertson Vj, Knobloch J, et al. Evaluation of two nested PCR assays for detection of Histoplasma capsulatum DNA in human tissue. J Clin Microbiol. 2002;40:1644-7.
7. Fugita S, Senda Y Nakaguchi S, Hashimoto T. Multiplex PCR Using Transcrebed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains. J Clin Microbiol. 2001;39:3617-22.