Standard methods for chemical ecology research in Apis mellifera

Journal of Apicultural Research

Volumn 52, Issue 4, 2013, Pages

  Torto, Baldwyn ^a   Carroll, Mark J ^b   Duehl, Adrian ^c   Fombong, Ayuka T ^a   Gozansky, Tamar Katzav ^d   Nazzi, Francesco ^e   Soroker, Victoria ^f   Teal, Peter E A ^c  

^a INTERNATIONAL CENTRE OF INSECT PHYSIOLOGY AND ECOLOGY ICIPE   (Kenya)

^b CARL HAYDEN BEE RESEARCH CENTER   (United States)

^c CENTER FOR MEDICAL   (United States)

^d OPEN UNIVERSITY OF ISRAEL   (Israel)

^e UNIVERSITY OF UDINE   (Italy)

^f VOLCANI CENTER   (Israel)

Author keywords

COLOSS BEEBOOK; EAG; Exocrine gland; Headspace; Honey bee; In vitro; QMP; Semiochemicals

Indexed keywords

EID: 84875386977     PISSN: 00218839     EISSN: 20786913     Source Type: Journal    
DOI: 10.3896/IBRA.1.52.4.06     Document Type: Review

References (110)

1. AGELOPOULOS, N; PICKETT, J A (1998) Headspace analysis in chemical ecology: effects of different sampling methods on ratios of volatile compounds present in headspace samples. Journal of Chemical Ecology 24(7):1161-1172. http://dx.doi.org/10.1023/A:1022442818196
2. ALAUX, C; ROBINSON, G E (2007) Alarm pheromone induces immediate-early gene expression and slow behavioural response in honey bees. Journal of Chemical Ecology 33(7):1346-1350. http://dx.doi.org/10.1007/s10886-007-9301-6
3. ARBOGAST, R T; TORTO, B; VANENGELSDORP, D; TEAL, P E A (2007) An effective trap and bait combination for monitoring the small hive beetle, Aethina tumida (Coleoptera: Nitidulidae). Florida Entomologist 90(2):404-406.
4. AUGUSTO, F; VALENTE, A L P (2002) Applications of solid-phase microextraction to chemical analysis of live biological samples. Trends in Analytical Chemistry 21(6-7):428-438. http://dx.doi.org/10.1016/S0165-9936 (02) 00602-7
5. BAKER, T C; WILLIS, M A; HAYNES, K F; PHELAN, P L (1985) A pulsed cloud of sex pheromone elicits upwind flight in male moths. Physiological Entomology 10:257-265.
6. BLAND, J M; OSBRINK, W L A; CORNELIUS, M L; LAX, A R; VIGO, C B (2001) Solid-phase microextraction for the detection of termite cuticular hydrocarbons. Journal of Chromatography A 932:119-127. http://dx.doi.org/10.1016/S0021-9673 (01) 01239-0
7. BLOMQUIST, G (2010) Structure and analysis of insect hydrocarbons. In Blomquist G J; Bagneres A G (Eds) Insect hydrocarbons: biology, biochemistry and chemical ecology. Cambridge University Press, UK. 491 pp.
8. BLOMQUIST, G J; HOWARD, R W; MCDANIEL, C A; REMALEY, S; DWYER, L A; NELSON, D R (1980) Application of methoxymercuration-demercuration followed by mass spectrometry as a convenient microanalytical technique for double-bond location in insect-derived alkenes. Journal of Chemical Ecology 6(1):257-269. http://dx.doi.org/10.1007/BF00987544
9. BLOMQUIST, G J; NELSON, D R; RENOBLES DE, M (1987) Chemistry, biochemistry, and physiology of insect cuticular lipids. Archives of Insect Biochemistry and Physiology 6(4):227-265. http://dx.doi.org/10.1002/arch. 940060404
10. BLUM, M S (1992) Honey bee pheromones. In Graham, J M (Ed.). The hive and the honey bee. Dadant; Hamilton, IL, USA. pp 373-400.
11. BLUM, M S; FALES, H M (1988) Eclectic chemisociality of the honey bee: a wealth of behaviors, pheromones, and exocrine glands. Journal of Chemical Ecology 14(11):2099-2107. http://dx.doi.org/10.1007/BF01014252
12. BOCH, R; SHEARER, D A; STONE, B C (1962) Identification of iso-amyl acetate as an active component in the sting pheromone of the honey bee. Nature 195(4845):1018-1020. http://dx.doi.org/10.1038/1951018b0
13. BOCH, R; SHEARER, D A (1964) Identification of nerolic and geranic aicds in the Nassanoff pheromone of the honey bee. Nature 202(4930):320-321. http://dx.doi.org/10.1038/202320a0
14. BREED, M D (1998) Recognition pheromones of the honey bee. BioScience 48(6):463-470. http://dx.doi.org/10.2307/1313244
15. BREED, M D; WILLIAMS, K R; FEWELL, J H (1988) Comb wax mediates the acquisition of nest-mate recognition cues in honey bees. Proceedings of the National Academy of Sciences 85(22):8766-8769.
16. CARLSON, D A; BOLTON, A B (1984) Identification of Africanized and European bees using extracted hydrocarbons. Bulletin of the Entomological Society of America, 30(1):32-35.
17. CARLSON, D A; ROAN, C; YOST, R A; HECTOR, J (1989) Dimethyl disulfide derivatives of long chain alkenes, alkadienes, and alkatrienes for gas chromatography/mass spectrometry. Analytical Chemistry 61(14):1564-1571. http://dx.doi.org/10.1021/ac00189a019
18. CARLSON, D A; BERNIER, U R; SUTTON, B D (1998) Elution patterns from capillary GC for methyl-branched alkanes. Journal of Chemical Ecology 24(11):1845-1865. http://dx.doi.org/10.1023/A:1022311701355
19. CARROLL, M J; DUEHL, A J (2012) Collection of volatiles from honey bee larvae and adults enclosed on brood frames. Apidologie 43(6):715-730. http://dx.doi.org/10.1007/s13592-012-0153-x
20. CHRISTENSEN, T A (2004) Methods in insect sensory neuroscience. CRC Press; Florida, USA. 435 pp.
21. D'ALESSANDRO, M; TURLINGS, T C J (2006) Advances and challenges in the identification of volatiles that mediate interactions among plants and arthopods. Analyst 131(1):24-32. http://dx.doi.org/10.1039/B507589K
22. DANI, F R; JONES, G R; CORSI, S; BEARD, R; PRADELLA, D; TURILLAZZI, S (2005) Nest mate recognition cues in the honey bee: differential importance of cuticular alkanes and alkenes. Chemical Senses 30(1):1-13. http://dx.doi.org/10. 1093/chemse/bji040
23. DE HAZAN, M; LENSKY, Y; CASSIER, P (1989) Effect of queen honey bee (Apis mellifera L.) aging on her attractiveness to workers. Comparative Biochemistry and Physiology A 93(4):777-783. http://dx.doi.org/10.1016/0300-9629 (89) 90501-X
24. DEL PICCOLO, F; NAZZI, F; DELLA VEDOVA, G; MILANI, N (2010) Selection of Apis mellifera workers by the parasitic mite Varroa destructor using host cuticular hydrocarbons. Parasitology 137(6):967-973. http://dx.doi.org/10.1017/ S0031182009991867
25. DIETEMANN, V; NAZZI, F; MARTIN, S J; ANDERSON, D; LOCKE, B; DELAPLANE, K S; WAUQUIEZ, Q; TANNAHILL, C; FREY, E; ZIEGELMANN, B; ROSENKRANZ, P; ELLIS, J D (2013) Standard methods for varroa research. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume II: standard methods for Apis mellifera pest and pathogen research. Journal of Apicultural Research 52(1):http://dx.doi.org/10.3896/IBRA.1.52.1.09
26. DOR, R; KATZAV-GOZANSKY, T; HEFETZ, A (2005) Dufour's gland pheromone as a reliable signal for reproductive dominance among workers honey bee (Apis mellifera). Behavioural Ecology and Sociobiology 58(3):270-276. http://dx.doi.org/10.1007/s00265-005-0923-9
27. DUCRET, A; TRANI, M; LORTIE, R (2008) General guidelines for authors for submission of manuscripts that contain identifications and shntheses of compounds. Journal of Chemical Ecology 34(8):984-986.
28. EVANS, J D; SCHWARZ, R S; CHEN, Y P; BUDGE, G; CORNMAN, R S; DE LA RUA, P; DE MIRANDA, J R; FORET, S; FOSTER, L; GAUTHIER, L; GENERSCH, E; GISDER, S; JAROSCH, A; KUCHARSKI, R; LOPEZ, D; LUN, C M; MORITZ, R F A; MALESZKA, R; MUÑOZ, I; PINTO, M A (2013) Standard methodologies for molecular research in Apis mellifera. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(4):http://dx.doi.org/10.3896/IBRA.1.52.4.11
29. FRANCKE, W; LÜBKE, G; SCHRÖDER, W; RECKZIEGEL, A; IMPERATRIZ-FONSECA, V; KLEINERT, A; ENGELS, E; HARTFELDER, K; RADKE, R; ENGELS, W (2000). Identification of oxygen containing volatiles in cephalic secretions of workers of Brazilian stingless bees. Journal of Brazilian Chemical Society 11(6):562-571.
30. FREE, J B (1987) Pheromones of social bees. Chapman and Hall; London, UK. 218 pp.
31. FRANCIS, B R; BLANTON, W E; NUNAMAKER, R A (1985) Extractable surface hydrocarbons of workers and drones of the genus Apis. Journal of Apicultural Research 24(1):13-26.
32. FRANCIS, B R; BLANTON, W E; LITTLEFIELD, J L; NUNAMAKER, R A (1989) Hydrocarbons of the cuticle and haemolymph of the adult honey bee (Hymenoptera: Apidae). Annals of the Entomological Society of America 82(4):486-493.
33. GENERSCH, E; GISDER, S; HEDTKE, K; HUNTER, W B; MÖCKEL, N; MÜLLER, U (2013) Standard methods for cell cultures in Apis mellifera research. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(1):http://dx.doi.org/10.3896/IBRA.1.52.1.02
34. GILLEY, D C; DEGRANDI-HOFFMAN, G; HOOPER, J E (2006) Volatile compounds emitted by live European honey bee (Apis mellifera L.) queens. Journal of Insect Physiology 52(5):520-527. http://dx.doi.org/10.1016/j.jinsphys.2006.01.014
35. GRAMACHO, K P; SPIVAK, M (2003) Differences in olfactory sensitivity and behavioral responses among honey bees bred for hygienic behavior. Behavioral Ecology and Sociobiology 54(5):472-479. http://dx.doi.org/10.1007/s00265-003- 0643-y
36. HANDLEY, A J; ADLARD, E R (2005) Gas chromatographic techniques and applications. CRC Press; Boca Raton, FL, USA.
37. HARE, J D (1998) Bioassay methods in terrrestrial invertebrates. In Millar, J G; Haynes, K F (Eds). Methods in chemical ecology: bioassay methods. Kluwer Academic Publishers; Boston, USA. pp 212-271.
38. HASEGAWA, M; ASANUMA, S; FUJIYUKI, T; KIYA T; SASAKI, T; ENDO D; MARIOKA, M; KUBO, T (2009) Differential gene expression in the mandibular glands of queen and worker honey bees, Apis mellifera. Insect Biochemistry and Molecular Biology 39(10):661-667. http://dx.doi.org/10.1016/j.ibmb.2009.08.001
39. HARTFELDER, K; GENTILE BITONDI, M M; BRENT, C; GUIDUGLI-LAZZARINI, K R; SIMÕES, Z L P; STABENTHEINER, A; DONATO TANAKA, É; WANG, Y (2013) Standard methods for physiology and biochemistry research in Apis mellifera. In V Dietemann; J D Elis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(1):http://dx.doi.org/10.3896/IBRA.1.52.1.06
40. HEATH, R R; MANUKIAN, A (1994) An automated system for use in collecting volatile chemicals released from plants. Journal of Chemical Ecology 20(3):593-608. http://dx.doi.org/10.1007/BF02059600
41. HUMAN, H; BRODSCHNEIDER, R; DIETEMANN, V; DIVELY, G; ELLIS, J; FORSGREN, E; FRIES, I; HATJINA, F; HU, F-L; JAFFÉ, R; KÖHLER, A; PIRK, C W W; ROSE, R; STRAUSS, U; TANNER, G; TARPY, D R; VAN der STEEN, J J M; VEJSNÆS, F; WILLIAMS, G R; ZHENG, H-Q (2013) Miscellaneous standard methods for Apis mellifera research. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(4):http://dx.doi.org/10.3896/IBRA.1.52.4.10
42. HUNT, G J; WOOD, K V; GUZMAN-NOVOA, E; LEE, H D; ROTHWELL, A P; BONHAM, C C (2003) Discovery of 3-methyl-3-buten-1-yl acetate, a new alarm component in the sting apparatus of Africanized honey bees. Journal of Chemical Ecology 29(2):453-463. http://dx.doi.org/10.1023/A:1022694330868
43. KAATZ, H H; HAGEDORN, H H; ENGELS, W (1985) Culture of honey bee organs: development of a new medium and the importance of tracheation in vitro. Ce lular and Developmental Biology 21:347-352.
44. KAMINSKI, L A; SLESSOR, K N; WINSTON, M L; HAY, N W; BORDEN, J H (1990) Honey bee response to queen mandibular pheromone in laboratory bioassays. Journal of Chemical Ecology 16(3):841-850. http://dx.doi.org/10.1007/BF01016494
45. KATZAV-GOZANSKY, T; SOROKER, V; HEFETZ, A; COJOCARU, M; ERDMANN, D H; FRANCKE, W (1997) Plasticity of caste-specific Dufour's gland secretion in the honey bee (Apis mellifera L.). Naturwissenschaften 84(4):238-241. http://dx.doi.org/10.1007/s001140050386
46. KATZAV-GOZANSKY, T; SOROKER, V; HEFETZ, A (2000) Plasticity in caste-related exocrine secretion biosynthesis in the honey bee (Apis mellifera). Journal of Insect Physiology 46(6):993-998. http://dx.doi.org/10.1016/S0022- 1910 (99) 00209-7
47. KATZAV-GOZANSKY, T; SOROKER, V; IBARRA, F; FRANCKE, W; HEFETZ, A (2001) Dufour's gland secretion of the queen honey bee (Apis mellifera): an egg discriminator pheromone or a queen signal? Behavioral Ecology and Sociobiology 51(1):76-86. http://dx.doi.org/10.1007/s002650100406
48. KATZAV-GOZANSKY, T; SOROKER, V; HEFETZ; A (2003) Honey bee egg laying workers mimics the queen signal. Insectes Sociaux 50(1):20-23. http://dx.doi.org/10.1007/s000400300003
49. KATZAV-GOZANSKY, T; BOULAY, R; SOROKER, V; HEFETZ, A (2004) Queen-signal modulation of worker pheromonal composition in honey bees. Proceedings of the Royal Society of London: Biological Sciences 271(1552):2065-2069. http://dx.doi.org/10.1098/rspb.2004.2839
50. KATZAV-GOZANSKY, T; HEFETZ, A; SOROKER, V; (2007) Brain modulation of Dufour's gland ester biosynthesis in vitro in the honey bee (Apis mellifera). Naturwissenschaften 94(5):407-411. http://dx.doi.org/10.1007/s00114-006-0206-y
51. KEELING, C I; SLESSOR, K N; HIGO, H A; WINSTON, M L (2003) New components of the honey bee (Apis mellifera L.) queen retinue pheromone. Proceedings of the National Academy of Sciences 100(8):4486-4491. http://dx.doi.org/10.1073/ pnas.0836984100
52. KOCHER, S D; RICHARD, F-J; TARPY, D R;. GROZINGER, C M (2009) Queen reproductive state modulates pheromone production and queen worker interactions in honey bees. Behavioral Ecology 20(5):1007-1014. http://dx.doi.org/10.1093/ beheco/arp090
53. KRAUS, B; VELTHUIS, H H W (1997) High humidity in the honey bee (Apis mellifera L.) brood nest limits reproduction of the parasitic mite Varroa jacobsoni Oud. Naturwissenschaften 84(5):217-218. http://dx.doi.org/10.1007/ s001140050382
54. MAISONNASSE, A; LENOIR, J C; COSTAGLIOLA, G; BESLAY, D; CHOTEAU, F; CRAUSER, D; BECARD, J M; PLETTNER, E; LECONTE, Y (2009) A scientific note on E-ß-ocimene, a new volatile primer pheromone that inhibits worker ovary development in honey bees. Apidologie 40(5):562-564. http://dx.doi.org/10.1051/ apido/2009024
55. MAISONNASSE, A; LENOIR, J C; BESLAY, D; CRAUSER, D; LECONTE, Y (2010) E-ß-Ocimene, a volatile brood pheromone involved in social regulation in the honey bee colony (Apis mellifera). PLoS ONE 5(10):e13531. http://dx.doi.org/10.1371/journal.pone.0013531
56. MALKA, O; KRUNGER, I; YEHESKEL, A; MORIN, S; HEFETZ, A (2009) The road to royalty-differential expression of hydroxylating genes in the mandibular glands of the honey bee. Federation of European Biochemical Societies Journal 276(19):5481-5490. http://dx.doi.org/10.1111/j.1742-4658.2009.07232.x
57. MALKA, O; SHNIEOR, S; HEFETZ, A; KATZAV-GOZANSKY, T (2007) Reversible royalty in worker honey bees under the queen influence. Behavioural Ecology and Sociobiology 61(3):465-473. http://dx.doi.org/10.1007/s00265-006-0274-1
58. MARTIN, S J; JONES, G R (2004) Conservation of bio synthetic pheromone pathways in honey bees Apis. Naturwissenschaften 91(5):232-236. http://dx.doi.org/10.1007/s00114-004-0517-9
59. MASTERMAN, R; ROSS, R; MESCE, K; SPIVAK, M (2001) Olfactory and behavioralresponse thresholds to odors of diseased brood differ between hygienic and non-hygienic honey bees (Apis mellifera L.). Journal of Comparative Physiology A 187(6):441-452. http://dx.doi.org/10.1007/s003590100216
60. MILLAR, J; HAYNES, K F (1998) Methods in chemical ecology: chemical methods. Chapman & Hall; USA. 420 pp.
61. MILLAR, J G; SIMS, J J (1998) Preparation, cleanup and preliminary fractionation of extracts. In Millar, J G; Haynes, K F (Eds). Methods in chemical ecology: bioassay methods. Kluwer Academic Publishers; Boston, USA. pp 2-37.
62. MORITZ, R; CREWE, R M (1991) The volatile emission of honey bee queens (Apis mellifera L). Apidologie 22(3):205-212. http://dx.doi.org/10.1051/apido: 19910304
63. NAUMANN, K; WINSTON, M L; SLESSOR, K N; PRESTWICH, G D; WEBSTER, F X (1991) Production and transmission of queen (Apis mellifera L.) mandibular gland pheromone. Behavioral Ecology and Sociobiology 29(5):321-332. http://dx.doi.org/10.1007/BF00165956
64. NEUMANN, P; PIRK, C W W; SCHÄFER, M O; EVANS, J D; PETTIS, J S; TANNER, G; WILLIAMS, G R; ELLIS, J D (2013) Standard methods for small hive beetle research. In V Dietemann; J D Ellis, P Neumann (Eds) The COLOSS BEEBOOK: Volume II: Standard methods for Apis mellifera pest and pathogen research. Journal of Apicultural Research 52(4):http://dx.doi.org/10.3896/IBRA.1.52.4.19
65. NAZZI, F; MILANI N; DELLA VEDOVA, G (2002) (Z)-8-Heptadecene from infested cells reduces the reproduction of Varroa destructor under laboratory conditions. Journal of Chemical Ecology 28(11):2181-2190. http://dx.doi.org/10. 1023/A:1021041130593
66. NAZZI, F; MILANI, N; DELLA VEDOVA, G (2004) A semiochemical from larval food influences the entrance of Varroa destructor into brood cells. Apidologie 35(4):403-410. http://dx.doi.org/10.1051/apido:2004023
67. NAZZI, F; BORTOLOMEAZZI, R; VEDOVA, G D; DEL PICCOLO, F; ANNOSCIA, D; MILANI, N (2009) Octanoic acid confers to royal jelly varroa-repellent properties. Naturwissenschaften 96(2):309-314. http:dx.doi.org/10.1007/s00114- 008-0470-0
68. NÚÑEZ, A J; GONZALEZ, L F; JANAK, J (1984) Pre-concentration of headspace volatiles for trace organic analysis by gas chromatography. Journal of Chromatography 300(1):127-162. http://dx.doi.org/10.1016/S0021-9673 (01) 87583-X
69. PANKIW, T (2004) Cued in: honey bee pheromones as information flow and collective decision-making. Apidologie 35(2):217-226. http://dx.doi.org/10.1051/ apido:2004009
70. PANKIW, T; HUANG, Z-Y; WINSTON, M L; ROBINSON, G E (1998) Queen mandibular gland pheromone influences worker honey bee (Apis mellifera L.) foraging ontogeny and juvenile hormone titers. Journal of Insect Physiology 44(7-8):685-282. http://dx.doi.org/10.1016/S0022-1910 (98) 00040-7
71. PANKIW, T; WINSTON, M L; SLESSOR, K N (1994) Variation in worker response to honey bee (Apis mellifera L.) queen mandibular pheromone (Hymenoptera: Apidae). Journal of Insect Behaviour 7(1):1-15. http://dx.doi.org/10.1007/ BF01989823
72. PANKIW, T; WINSTON, M; FONDRK, M K; SLESSOR, K N (2000) Selection on worker honey bee responses to queen pheromone (Apis mellifera L.). Naturwissenschaften 87(11):487-490. http://dx.doi.org/10.1007/s001140050764
73. PICKETT, J A; WILLIAMS, I H; MARTIN, A P; SMITH, M C (1980) Nasonov pheromone of the honey bee Apis mellifera L. (Hymenoptera: Apidae) Part I. Chemical characterization. Journal of Chemical Ecology 6(2):425-432. http://dx.doi.org/10.1007/BF01402919
74. PLETTNER, E; SUTHERLAND, G R J; SLESSOR, K N; WINSTON, M L (1995) Why not be a queen? Regioselectivity in mandibular secretions of honey bee castes. Journal of Chemical Ecology 21(6):1017-1029. http://dx.doi.org/10.1007/ BF02033805
75. PLETTNER, E; SLESSOR, K N; WINSTON, M L; OLIVER, J E (1996) Caste-selective pheromone biosynthesis in honey bees. Science 271(5257):1851-1853. http://10.1126/science.271.5257.1851
76. RAGUSO, R A; PELLMYR, O (1998) Dynamic headspace analysis of floral volatiles: a comparison of methods. Oikos 81(2):238-254.
77. REYMENT, R A (1989) Compositional data analysis. Terra Nova 1:29-34. http://dx.doi.org/10.1111/j.1365-3121.1989.tb00322.x
78. RICKLI, M; DIEHL, P A; GUERIN, P M. (1994) Cuticle alkanes of honey bee larvae mediate arrestment of bee parasite Varroa jacobsoni. Journal of Chemical Ecology 20(9):2437-2453. http://dx.doi.org/10.1007/BF02033212
79. RAFAELI, A (2009) Pheromone biosynthesis activating neuropeptide (PBAN): Regulatory role and mode of action. General Comparative Endocrinology 162(1):69-78. http://dx.doi.org/10.1016/j.ygcen. 2008.04.004
80. ROBINSON, G E (1985) Effects of a juvenile hormone analogue on honey bee foraging behaviour and alarm pheromone production. Journal of Insect Physiology 31(4):277-282. http://dx.doi.org/10.1016/0022-1910 (85) 90003-4
81. ROBINSON, G E A; HUANG, Z-H (1998) Colony integration in honey bees: genetic, endocrine and social control of division of labour. Apidologie 29(1-2):159-170. http://dx.doi.org/10.1051/apido:19980109
82. ROMEO, J T (2009) New SPME guidelines. Journal of Chemical Ecology 35(12):1383. http://dx.doi.org/10.1007/s10886-009-9733-2.
83. SALVY, M; MARTIN, C; BAGNÈRES, A G; PROVOST, E; ROUX, M; LECONTE, Y; CLÉMENT, J L (2001) Modifications of the cuticular hydrocarbon profile of Apis mellifera worker bees in the presence of the ectoparasitic mite Varroa jacobsoni in brood cells. Parasitology 122(2):145-159. http://dx.doi.org/10. 1017/S0031182001007181
84. SCHEINER, R; ABRAMSON, C I; BRODSCHNEIDER, R; CRAILSHEIM, K; FARINA, W; FUCHS, S; GRÜNEWALD, B; HAHSHOLD, S; KARRER, M; KOENIGER, G; KOENIGER, N; MENZEL, R; MUJAGIC, S; RADSPIELER, G; SCHMICKLl, T; SCHNEIDER, C; SIEGEL, A J; SZOPEK, M; THENIUS, R (2013) Standard methods for behavioural studies of Apis mellifera. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(4):http://dx.doi.org/10.3896/IBRA.1.52.4.04
85. SCHMITT, T; HERZNER, G; WECKERLE, B; SCHREIER, P; STROHM, E (2007) Volatiles of foraging honey bees Apis mellifera (Hymenoptera: Apidae) and their potential role as semiochemicals. Apidologie 38(2):164-170. http://dx.doi.org/ 10.1051/apido:2006067
86. SCHNEIDER, D (1957). Elektrophysiologische untersuchungen von chemorezeptoren und mechanorezeptoren der antenne des seidenspinners Bombyx mori L. Zeitschrift fur Vergleichende Physiologie 40:8-41. http://dx.doi.org/10. 1007/BF00298148
87. SCHÖNING, C S; GISDER, S; GEISELHARDT, S; KRETSCHMANN, I; BIENEFELD, K; HILKER, M; GENERSCH, E (2012) Evidence for damage-dependent hygienic behaviour towards Varroa destructorparasitised brood in the western honey bee, Apis mellifera. The Journal of Experimental Biology 215(2):264-271. http://dx.doi.org/10.1242/jeb.062562
88. SEELEY, T D (1982) Adaptive significance of the age polytheism schedule in honey bee colonies. Behavioural Ecology and Sociobiology 11(4):287-293. http://dx.doi.org/10.1007/BF00299306
89. SEIDELMANN, K; WEINERT, H; FERENZ, H-J (2003) Wings and legs are production sites for desert locust courtship-inhibition pheromone, phenyl acetonitrile Journal of Insect Physiology 49(12):1125-1133. http://dx.doi.org/ 10.1016/j.jinsphys.2003.08.005
90. SKOOG, D A; HOLLER, F J; CROUCH, S R (2007) Principles of instrumental analysis, (6th Ed.). Brooks/Cole, Thomson Learning; Belmont, CA, USA.
91. SLESSOR, K N; KAMINSKI, L A; KING, G G S; BORDEN, H J; WINSTON, M L (1988) Semiochemical basis of the retinue response to queen honey bees. Nature 332(6162):354-356. http://dx.doi.org/10.1038/332354a0
92. SLESSOR, K N; WINSTON, M; LE CONTE, Y (2005) Pheromone communication in the honey bee (Apis mellifera L.). Journal of Chemical Ecology 31(11):2731-2745. http://dx.doi.org/10.1007/s10886-005-7623-9
93. SONNET, P E (1984) Tabulation of selected methods of syntheses that are frequently employed for insect sex pheromones, emphasizing the literature of 1977-1982. In Hummel, H E; Miller, T A (Eds). Techniques in pheromone research. Springer-Verlag; New York, USA.
94. SOROKER, V; HEFETZ, A (2000) Hydrocarbon site of synthesis and circulation in the desert ant Cataglyphis niger. Journal of Insect Physiology 46(7):1097-1102. http://dx.doi.org/10.1016/S0022-1910 (99) 00219-X
95. SOROKER, V; RAFAELI, A (1989) In vitro hormonal stimulation of acetate incorporation by Heliothis armigera pheromone glands. Journal of Insect Biochemistry 19(1):1-5. http://dx.doi.org/10.1016/0020-1790 (89) 90002-4
96. STANLEY-SAMUELSON, D W; JURENKA, R A; CRIPPS, C; BLOMQUIST, G J; de RENOBLES, M (1988) Fatty acids in insects: composition, metabolism, and biological significance. Archives of Insect Biochemistry and Physiology 9(1):1-33. http://dx.doi.org/10.1002/arch.940090102
97. SWANSON, J A; TORTO, B; KELLS, S A; MESCE, K A; TUMLINSON, J H, SPIVAK, M (2009) Odorants that induce hygienic behavior in honey bees: identification of volatile compounds in chalkbroodinfected honey bee larvae. Journal of Chemical Ecology 35(9):1108-1116. http://dx.doi.org/10.1007/s10886-009-9683-8
98. SYNTECH (2004) Electroantennography: a practical introduction. Hilversum; Netherlands.
99. TEAL, P E A; TORTO, B; BOUCIAS, D; TUMLINSON, J H (2006) In-hive trap and attractant composition for the control of the small hive beetle, Aethina tumida. US Patent, 20060141904. 29 June 2006.
100. THOLL, D; BOLAND, W; HANSEL, A; LORETO, F, RÖSE, U S R; SCHNITZLER, J-P (2006) Practical approaches to plant volatile analysis. The Plant Journal 45(4):540-560. http://dx.doi.org/10.1111/j.1365-313X.2005.02612.x
101. THOM, C; GILLEY, D C; HOOPER, J; ESCH, H E (2007) The scent of the waggle dance. PLoS Biology 5(9):1862-1867. http://dx.doi.org/10.1371/journal.pbio. 0050228
102. TORTO, B; BOUCIAS, D G; ARBOGAST, R T; TUMLINSON, J H; TEAL, P E A (2007a) Multi-trophic interaction facilitates parasite-host relationship between an invasive beetle and the honey bee. Proceedings of the National Academy of Science, USA 104(20):8374-8378. http://dx.doi.org/10.1073/pnas.0702813104
103. TORTO, B; ARBOGAST, R T; VAN ENGELSDORP, D; WILLMS, S; PURCELL, D; BOUCIAS, D; TUMLINSON, J H; TEAL, P E A (2007b) Trapping of Aethina tumida Murray (Coleoptera: Nitidulidae) from Apis mellifera L. (Hymenoptera: Apidae) colonies with an in-hive baited trap. Environmental Entomology 36(5):1018-1024. http://dx.doi.org/10.1603/0046-225X (2007) 36[1018:TOATMC] 2.0. CO;2
104. TORTO, B; SUAZO, A; ALBORN, H; TUMLINSON, J H; TEAL, P E A (2005) Response of the small hive beetle (Aethina tumida) to a blend of chemicals identified from honey bee (Apis mellifera) volatiles. Apidologie 36(4):523-532. http://dx.doi.org/10.1051/apido:2005038
105. TSFADIA, O; AZRIELI, A; FALECH, L; ZADA A; ROELOFS W; RAFAELI, A (2008) Pheromone biosynthesis pathway: PBAN-regulated ratelimiting steps and deferential expression of desaturase genes in moth species. Insect Biochemistry and Molecular Biology 38(5):552-567. http://dx.doi.org/10.1016/j.ibmb.2008.01. 005
106. WILLIAMS, G R; ALAUX, C; COSTA, C; CSÁKI, T; DOUBLET, V; EISENHARDT, D; FRIES, I; KUHN, R; MCMAHON, D P; MEDRZYCKI, P; MURRAY, T E; NATSOPOULOU, M E; NEUMANN, P; OLIVER, R; PAXTON, R J; PERNAL, S F; SHUTLER, D; TANNER, G; VAN der STEEN, J J M; BRODSCHNEIDER, R (2013) Standard methods for maintaining adult Apis mellifera in cages under in vitro laboratory conditions. In V Dietemann; J D Ellis; P Neumann (Eds) The COLOSS BEEBOOK, Volume I: standard methods for Apis mellifera research. Journal of Apicultural Research 52(1):http://dx.doi.org/10.3896/IBRA.1.52.1.04
107. WINSTON, M L (1987) The biology of the honey bee. Harvard University Press; Cambridge, Massachusetts, USA. 281 pp.
108. WOSSLER, T C; CREWE, R M (1999) The releaser effects of the tergal gland secretion of queen honey bees (Apis mellifera). Journal of Insect Behavior 12(3):343-351. http://dx.doi.org/10.1023/A:1020839505622
109. WOSSLER, T C; JONES, G E; ALLSOP, M H; HEPBURN, R (2006) Virgin queen mandibular gland signals of Apis mellifera capensis change in age and affect honey bee worker response Journal of Chemical. Ecology 32(5):1043-1056. http://dx.doi.org/10.1007/s10886-006-9053-8
110. ZABARAS, D; WYLLIE, S G (2002) Rearrangement of p-menthane terpenes by Carboxen during HS-SPME. Journal of Separation Science 25(10-11):685-690. http://dx.doi.org/10.1002/1615-9314 (20020701) 25:10/11<685::AID- JSSC685>3.0. CO;2-9