Detection and characterization of an unknown rice event in Basmati rice products

European Food Research and Technology

Volumn 236, Issue 4, 2013, Pages 715-723

  Reiting, Ralf ^a   Grohmann, Lutz ^b   Moris, Gilbert ^c   Mäde, Dietrich ^d  

^a Hessisches Landeslabor   (Germany)

^b Bundesamt fur Verbraucherschutz und Lebensmittelsicherheit   (Germany)

^c LABORATOIRE NATIONAL DE SANTÉ   (Luxembourg)

^d Landesamt Verbraucherschutz S   (Germany)

Author keywords

Construct specific PCR; Cry2A; Detection method; GMO; Real time PCR; Transgenic rice

Indexed keywords

DNA SEQUENCES; GENES; PLANTS (BOTANY); SCREENING;

CONSTRUCT-SPECIFIC PCR; CRY2A; DETECTION METHODS; REAL-TIME PCR; TRANSGENIC RICE;

POLYMERASE CHAIN REACTION;

HEXAPODA; ZEA MAYS;

EID: 84875376389     PISSN: 14382377     EISSN: 14382385     Source Type: Journal    
DOI: 10.1007/s00217-013-1928-7     Document Type: Article

References (46)

1. Beintema N, Raitzer D, Dobermann A, Pray C, Sanint L, Wopereis M (2010) Positioning rice research globally: investments, institutional arrangements, and emerging challenges. In: rice in the global economy strategic research and policy issues for food security. Chap 4. 3. In: Pandey S, Byerlee D, Dawe D, Dobermann A, Mohanty S, Rozelle S, and Hardy B (eds.) Rice in the global economy: strategic research and policy issues for food security. International Rice Research Institute, Los Baños (Philippines), p 477.
2. Escaler M, Teng P, Powell A (2012) Challenges of harmonization of agricultural biotechnology regulatory systems across the APEC economies. Biosafety 1: 3. doi: 10. 4172/bs. 1000101.
3. James C (2011) Global status of commercialized biotech/GM Crops: 2011. ISAAA Brief No. 43. ISAAA: Ithaca, NY.
4. Frutos R, Rang C, Royer M (1999) Managing insect resistance to plants producing Bacillus thuringiensis toxins. Crit Rev Biotechnol 19(3): 227-276.
5. Zhang Q (2007) Strategies for developing green super rice. PNAS 104: 16402-16409.
6. Tan T, Zhan J, Chen C (2011) The impact of commercialization of GM Rice in China. Am Eurasian J Agric Environ Sci 10: 296-299.
7. Mäde D, Degner C, Grohmann L (2006) Detection of genetically modified rice: a construct-specific real-time PCR method based on DNA sequences from transgenic Bt rice. Eur Food Res Technol 224: 271-278.
8. Akiyama H, Sasaki N, Sakata K, Ohmori K, Toyota A, Kikuchi Y, Watanabe T, Furui S, Kitta K, Maitani T (2007) Indicated detection of two unapproved transgenic rice lines contaminating vermicelli products. J Agric Food Chem 55: 5942-5947.
9. Reiting R, Grohmann L, Mäde D (2010) A testing cascade for the detection of genetically modified rice by real-time PCR in food and its application for detection of an unauthorized rice line similar to KeFeng6. J Verbr Lebensm 5: 185-188.
10. Anonymus (2011) Final Report of an Audit Carried out in China from 29 March to 08 April in Order to evaluate the control systems for genetically modified organisms (GMOs) in respect of seed, food and feed intended for export to the EU. DG(SANCO) 2011-6208-MR FINAL. European Commission. Health and Consumers Directorate-General. Directorate F-Food and Veterinary Office.
11. Regulation (EC) No 1829/2003 of the European parliament and of the council of 22 September 2003 on genetically modified food and feed. Off J Eur Union L268/1.
12. Commission Decision of 3 April 2008 on emergency measures regarding the unauthorised genetically modified organism 'Bt63' in rice products (2008/289/EC). Off J Eur Union 96: 29-34.
13. Commission implementing Decision of 22 December 2011 on emergency measures regarding unauthorised genetically modified rice in rice products originating from China and repealing Decision 2008/289/EC (2011/884/EU).
14. RASFF (2011) 2011. 1646 https://webgate. ec. europa. eu/rasff-window/portal/index. cfm?event=notificationDetail& NOTIF_REFERENCE=2011. 1646.
15. RASFF (2012) 2012. 0017; https://webgate. ec. europa. eu/rasff-window/portal/index. cfm?event=notificationDetail& NOTIF_REFERENCE=2012. 0017.
16. RASFF (2012) 2012. 0041; https://webgate. ec. europa. eu/rasff-window/portal/index. cfm?event=notificationDetail& NOTIF_REFERENCE=2012. 0041.
17. RASFF (2012) 2012. 0252; https://webgate. ec. europa. eu/rasff-window/portal/index. cfm?event=notificationDetail& NOTIF_REFERENCE=2012. 0252.
18. RASFF (2012) 2012. 0388; https://webgate. ec. europa. eu/rasff-window/portal/index. cfm?event=notificationDetail& NOTIF_REFERENCE=2012. 0388.
19. Grohmann L, Mäde D (2009) Detection of genetically modified rice: collaborative validation study of a construct-specific real-time PCR method for detection of transgenic Bt rice. Eur Food Res Technol 228: 497-500.
20. Hernandez M, Esteve T, Pla M (2005) Real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. J Agric Food Chem 53: 7003-7009.
21. Zeitler R, Pietsch K, Waiblinger H-U (2002) Validation of real-time PCR methods for the quantification of transgenic contaminations in rape seed. Eur Food Res Technol 214: 346-351.
22. Mazzara M, Grazioli E, Savini C, van Den Eede G (2006) Event-specific method for the quantitation of rice line LLRICE62 using real-time PCR-validation report and protocol-sampling and DNA extraction of Rice Online Publication. doi: 10. 2788/31940.
23. Anonymus (2005) Foodstuffs-methods of analysis for the detection of genetically modified organisms and derived products-quantitative nucleic acid based methods. International Organization for Standardization, Geneva Switzerland ISO 21570: 2005.
24. Fatima F (2001) Studies on expression of foreign genes in Rice (Oryza sativa L.) http://eprints. hec. gov. pk/529/1/327. html. htm.
25. Anonymus (2006) Sammlung von Untersuchungsverfahren nach § 64 LFGB; L29. 00-9 (September 2006): Qualitativer Nachweis modifizierter DNA-Sequenzen in Papaya-Ring-Spot-Virus-resistenter Papaya (Carica papaya) Konstrukt spezifisches Verfahren.
26. Altschul S, Gish W, Miller W, Myers E, Lipman D (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
27. Babekova R, Funk T, Pecoraro S, Engel K-H, Busch U (2009) Development of an event-specific real-time PCR detection method for the transgenic Bt rice line KMD1. Eur Food Res Technol 228: 707-716.
28. Wang W, Zhu T, Lai F, Fu Q (2011) Event-specific qualitative and quantitative detection of transgenic rice Kefeng-6 by characterization of the transgene flanking sequence. Eur Food Res Technol 232: 297-305. doi: 10. 1007/s00217-010-1389-1.
29. Waiblinger H-U, Ernst B, Anderson A, Pietsch K (2008) Validation and collaborative study of a P35S and T-nos duplex real-time screening method to detect genetically modified organisms in food products. Eur Food Res Technol 226: 1221-1228.
30. Maqbool S, Husnain T, Riazuddin S, Masson L, Christou P (1998) Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M7 using the novel delta-endotoxin cry2A Bacillus thuringiensis gene. Mol Breed 4: 501-507.
31. Chen H, Tang W, Xu C, Li X, Lin Y, Zhang Q (2005) Transgenic indica rice plants harboring a synthetic cry2A gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests. Theor Appl Genet 111: 1330-1337.
32. Kumar P. A. (2007). Synthetic cry2Aa gene from Bacillus thuringiensis. Acc. EU109565.
33. Ferre J, van Rie J (2002) Biochemistry and genetics of insect resistance to Bacillus thuringiensis. Annu Rev Entomol 47: 501-533.
34. Maqbool S, Christou P (1999) Multiple traits of agronomic importance in transgenic indica rice plants: analysis of transgene integration patterns, expression levels and stability. Mol Breed 5: 471.
35. Maqbool S, Riazuddin S, Loc N, Gatehouse A, Gatehouse J, Christou P (2001) Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Mol Breed 7: 85-93.
36. Yang Z, Chen H, Tang W, Hua H, Lin Y (2011) Development and characterisation of transgenic rice expressing two Bacillus thuringiensis genes. Pest Manag Sci 67: 414-422.
37. Tabashnik B, Dennehy T, Sims M, Larkin K, Head G, Moar W, Carriere Y (2002) Control of resistant pink bollworm (Pectinophora gossypiella) by transgenic cotton that produces Bacillus thuringiensis toxin Cry2Ab. Appl Environ Microb 68: 3790-3794.
38. Bakhsh A, Rao A, Shahid A, Husnain T, Riazuddin S (2009) Insect resistance and risk assessment studies in advance lines of Bt cotton harbouring Cry1Ac and Cry2A genes. Am Eurasian J Agric Environ Sci 6(1): 01-11.
39. Riaz N, Husnain T, Fatima T, Makhdoom R, Bashir K, Masson L, Altosaar I, Riazuddin S (2006) Development of indica Basmati rice harbouring two insecticidal genes for sustainable resistance against lepidopteran insects. S Afr J Bot 72: 217-223.
40. Bashir K, Husnain T, Fatima T, Latif Z, Mehdi S, Riazuddin S (2004) Field evaluation and risk assessment of transgenic indica Basmati rice. Mol Breed 134: 301-312.
41. Bashir K, Husnain T, Fatima T, Riaz N, Makhdoom R, Riazuddin S (2005) Novel indica Basmati line (B-370) expressing two unrelated genes of Bacillus thuringiensis is highly resistant to two lepidopteran insects in the field. Crop protection 24: 870-879.
42. Rahman M, Rashid H, Shahid A, Bashir K, Husnain T, Riazuddin S (2007) Insect resistance and risk assessment studies of advanced generations of Basmati rice expressing two genes of Bacillus thuringiensis. Elect J Biotechnol 10(2) Issue of April 15.
43. Code of Practice on Basmati rice (2005) http://riceassociation. fivesitestage. co. uk/download/10/Basmati. Rice. Code. of. Practice. pdf.
44. Wang W, Zhu T, Lai F, Fu Q (2012) Event-specific qualitative and quantitative detection of transgenic rice Kefeng-8 by characterization of the transgene flanking sequence. Eur Food Res Technol. doi: 10. 1007/s00217-011-1654-y.
45. Community Reference Laboratory for GM food and feed. (2006); http://gmo-crl. jrc. ec. europa. eu/LLRice601update. htm.
46. Yang L, Ding J, Zhang C, Jia J, Weng H, Liu W, Zhang D (2005) Estimating the copy number of trans genes in transformed rice by real-time quantitative PCR. Plant Cell Rep 23: 759-763.