Evaluation of PCR procedures for detecting and quantifying Leishmania donovani DNA in large numbers of dried human blood samples from a visceral leishmaniasis focus in Northern Ethiopia

BMC Infectious Diseases

Volumn 13, Issue 1, 2013, Pages

  Abbasi, Ibrahim ^a   Aramin, Samar ^a   Hailu, Asrat ^b   Shiferaw, Welelta ^b   Kassahun, Aysheshm ^b   Belay, Shewaye ^c   Jaffe, Charles ^a   Warburg, Alon ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b ADDIS ABABA UNIVERSITY   (Ethiopia)

^c MEKELLE UNIVERSITY   (Ethiopia)

Author keywords

Asymptomatic infections; Cohort study; DNA extraction; Ethiopia; kDNA PCR; Leishmania donovani; Visceral Leishmaniasis

Indexed keywords

ACETIC ACID; CHLOROFORM; DNA; INTERNAL TRANSCRIBED SPACER 1; LEISHMANIA DONOVANI DNA; PHENOL; SODIUM HYDROXIDE; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; ASYMPTOMATIC INFECTION; BLOOD SAMPLING; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DNA DETERMINATION; DNA EXTRACTION; DNA SEQUENCE; ETHIOPIA; HUMAN; KINETOPLASTIDA; LEISHMANIA DONOVANI; PARASITEMIA; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; VISCERAL LEISHMANIASIS;

ADULT; ANIMALS; BLOOD; CHILD; COHORT STUDIES; DESICCATION; DNA, KINETOPLAST; DNA, PROTOZOAN; ETHIOPIA; FEMALE; HUMANS; LEISHMANIA DONOVANI; LEISHMANIASIS, VISCERAL; MALE; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING;

EID: 84875332341     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/1471-2334-13-153     Document Type: Article

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