Misclassification of Plasmodium infections by conventional microscopy and the impact of remedial training on the proficiency of laboratory technicians in species identification

Malaria Journal

Volumn 12, Issue 1, 2013, Pages

  Obare, Peter ^a   Ogutu, Bernhards ^a,d   Adams, Mohammed ^b   Odera, James Sande ^a   Lilley, Ken ^c   Dosoo, David ^b   Adhiambo, Christine ^a   Owusu Agyei, Seth ^b   Binka, Fred ^d   Wanja, Elizabeth ^a   Johnson, Jacob ^a  

^a U S ARMY   (United States)

^b Kintampo Health Research Center   (Ghana)

^c AUSTRALIAN ARMY MALARIA INSTITUTE   (Australia)

^d Studies of the Epidemiology of Epilepsy in Demographic Surveillance Systems   (Ghana)

Author keywords

Microscopy; Misclassification; Morphology; Species; Training

Indexed keywords

ARTICLE; CLASSIFICATION; DENSITY; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; GIEMSA STAIN; GOOD LABORATORY PRACTICE; LABORATORY PERSONNEL; MALARIA; MALARIA FALCIPARUM; MICROSCOPY; MIXED INFECTION; MORPHOLOGY; NONHUMAN; PLASMODIUM; PLASMODIUM FALCIPARUM; PLASMODIUM MALARIAE; PLASMODIUM MALARIAE INFECTION; PLASMODIUM OVALE; PLASMODIUM OVALE MALARIA; PLASMODIUM VIVAX; PLASMODIUM VIVAX MALARIA; SPECIES IDENTIFICATION;

DIAGNOSTIC ERRORS; EDUCATION, MEDICAL, CONTINUING; HUMANS; LABORATORY PERSONNEL; MALARIA; MICROSCOPY; PARASITOLOGY; PLASMODIUM; PROFESSIONAL COMPETENCE; STAINING AND LABELING;

EID: 84875308220     PISSN: None     EISSN: 14752875     Source Type: Journal    
DOI: 10.1186/1475-2875-12-113     Document Type: Article

References (19)

1. Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections. Lee KS, Cox-Singh J, Singh B, Malar J 2009 8 73 10.1186/1475-2875-8-73 19383118
2. A review of malaria diagnostic tools: microscopy and rapid diagnostic test (RDT). Wongsrichanalai C, Barcus MJ, Muth S, Sutamihardja A, Wernsdorfer WH, AmJTrop Med Hyg 2007 77 119 127
3. Plasmodium knowlesi: reservoir hosts and tracking the emergence in humans and macaques. Lee KS, Divis PC, Zakaria SK, Matusop A, Julin RA, Conway DJ, Cox-Singh J, Singh B, PLoS Pathog 2011 7 1002015 10.1371/journal.ppat.1002015 21490952
4. Review of cases with the emerging fifth human malaria parasite, Plasmodium knowlesi. Kantele A, Jokiranta TS, Clin Infect Dis 2011 52 1356 1362 10.1093/cid/cir180 21596677
5. Random distribution of mixed species malaria infections in Papua New Guinea. Mehlotra RK, Lorry K, Kastens W, Miller SM, Alpers MP, Bockarie M, Kazura JW, Zimmerman PA, AmJTrop Med Hyg 2000 62 225 231
6. High prevalence of Plasmodium malariae infections in a Brazilian Amazon endemic area (Apiacás-Mato Grosso State) as detected by polymerase chain reaction. Scopel KK, Fontes CJ, Nunes ÁC, Horta MF, Braga ÉM, Acta Trop 2004 90 61 64 10.1016/j.actatropica.2003.11.002 14739024
7. Comparative population structure of Plasmodium malariae and Plasmodium falciparum under different transmission settings in Malawi. Bruce MC, Macheso A, McConnachie A, Molyneux ME, Malar J 2011 10 38 10.1186/1475-2875-10-38 21314950
8. Estimating the global clinical burden of Plasmodium falciparum malaria in 2007. Hay SI, Okiro EA, Gething PW, Patil AP, Tatem AJ, Guerra CA, Snow RW, PLoS Med 2010 7 1000290 10.1371/journal.pmed.1000290 20563310
9. Increased Plasmodium falciparum gametocyte production in mixed infections with P. malariae. Bousema JT, Drakeley CJ, Mens PF, Arens T, Houben R, Omar SA, Gouagna LC, Schallig H, Sauerwein RW, AmJTrop Med Hyg 2008 78 442 448
10. Plasmodium malariae: parasite and disease. Collins WE, Jeffery GM, Clin Microbiol Rev 2007 20 579 592 10.1128/CMR.00027-07 17934075
11. Plasmodium malariae and Plasmodium ovale: the bashful malaria parasites. Mueller I, Zimmerman PA, Reeder JC, Trends Parasitol 2007 23 278 283 10.1016/j.pt.2007.04.009 17459775
12. Two nonrecombining sympatric forms of the human malaria parasite Plasmodium ovale occur globally. Sutherland CJ, Tanomsing N, Nolder D, Oguike M, Jennison C, Pukrittayakamee S, Dolecek C, Hien TT, Do Rosario VE, Arez AP, Pinto J, Michon P, Escalante AA, Nosten F, Burke M, Lee R, Blaze M, Otto TD, Barnwell JW, Pain A, Williams J, White NJ, Day NP, Snounou G, Lockhart PJ, Chiodini PL, Imwong M, Polley SD, J Infect Dis 2010 201 1544 1550 10.1086/652240 20380562
13. Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite. Mueller I, Galinski MR, Baird JK, Carlton JM, Kochar DK, Alonso PL, del Portillo HA, Lancet Infect Dis 2009 9 555 566 10.1016/S1473-3099(09)70177-X 19695492
14. Failure to detect Plasmodium vivax in West and Central Africa by PCR species typing. Culleton RL, Mita T, Ndounga M, Unger H, Cravo PV, Paganotti GM, Takahashi N, Kaneko A, Eto H, Tinto H, Karema C, D'Alessandro U, Do Rosário V, Kobayakawa T, Ntoumi F, Carter R, Tanabe K, Malar J 2008 7 174 10.1186/1475-2875-7-174 18783630
15. Malaria. Snow RW, Omumbo JA, Disease and Mortality in Sub-Saharan Africa Washington (DC): World Bank, Jamison DT, Feachem RG, Makgoba MW, Bos ER, Baingana FK, Hofman KJ, Rogo KO, 2 2006 Chapter 14. http://www.ncbi.nlm.nih.gov/ books/NBK2286/
16. Multiple proportions and post hoc analysis http://www.statstodo.com/ MultiProp-Pgm.php
17. Use of standardized blood smear slide sets for competency assessment in the malaria microscopic diagnosis in the Peruvian Amazon. Rosas-Aguirre Á Gamboa D, Rodriguez H, Llanos-Zavalaga F, Aguirre K, Llanos-Cuentas A, Rev Peru Med Exp Salud Publica 2010 27 540 547 10.1590/S1726-46342010000400008 21308193
18. Detection and identification of malaria parasites: a review of proficiency test results and laboratory practices. Edson DC, Glick T, Massey LD, Lab Med 2010 4 719 723
19. Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum. Plasmodium vivax and Plasmodium knowlesi. Barber BE, William T, Grigg MJ, Yeo TW, Anstey NM, Malar J 2013 12 8 10.1186/1475-2875-12-8 23294844