Reconstitution of flagellar sliding

Methods in Enzymology

Volumn 524, Issue , 2013, Pages 343-369

  Alper, Joshua ^a   Geyer, Veikko ^a   Mukundan, Vikram ^a   Howard, Jonathon ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

Author keywords

Axoneme; Chlamydomonas; Dynein; Flagella; Gliding; Purification; Reactivation; Reconstitution; Sliding; Tubulin

Indexed keywords

ACETIC ACID; CHLOROPHYLL; DYNEIN ADENOSINE TRIPHOSPHATASE; POTASSIUM DIHYDROGEN PHOSPHATE;

ARTICLE; AUTOMATION; AXONEME; BIOTINYLATION; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CHLAMYDOMONAS; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; ENZYME ACTIVITY; IMAGE PROCESSING; NONHUMAN; OXIDATION; PH; PRIORITY JOURNAL; PROTEIN PURIFICATION; ROOM TEMPERATURE; SIGNAL NOISE RATIO;

EID: 84875247577     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-397945-2.00019-6     Document Type: Chapter

References (29)

1. S. Aoyama, and R. Kamiya Cyclical interactions between two outer doublet microtubules in split flagellar axonemes Biophysical Journal 89 2005 3261 3268
2. M. Bessen, R.B. Fay, and G.B. Witman Calcium control of waveform in isolated flagellar axonemes of Chlamydomonas The Journal of Cell Biology 86 1980 446 455
3. C.J. Brokaw, and D.J. Luck Bending patterns of Chlamydomonas flagella I. Wild-type bending patterns Cell Motility 3 1983 131 150
4. Z. Carvalho-Santos, J. Azimzadeh, J.B. Pereira-Leal, and M. Bettencourt-Dias Evolution: Tracing the origins of centrioles, cilia, and flagella The Journal of Cell Biology 194 2011 165 175
5. A. Furuta, T. Yagi, H.-A. Yanagisawa, H. Higuchi, and R. Kamiya Systematic comparison of in vitro motile properties between Chlamydomonas wild-type and mutant outer arm dyneins each lacking one of the three heavy chains The Journal of Biological Chemistry 284 2009 5927 5935
6. C. Gell, V. Bormuth, G.J. Brouhard, D.N. Cohen, S. Diez, and C.T. Friel Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy Methods in Cell Biology 95 2010 221 245
7. I.R. Gibbons Sliding and bending in sea urchin sperm flagella Symposia of the Society for Experimental Biology 35 1982 225 287
8. U.W. Goodenough, B. Gebhart, V. Mermall, D.R. Mitchell, and J.E. Heuser High-pressure liquid chromatography fractionation of Chlamydomonas dynein extracts and characterization of inner-arm dynein subunits Journal of Molecular Biology 194 1987 481 494
9. D.S. Gorman, and R.P. Levine Cytochrome f and plastocyanin: Their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii Proceedings of the National Academy of Sciences of the United States of America 54 1965 1665 1669
10. J. Howard, A.J. Hudspeth, and R.D. Vale Movement of microtubules by single kinesin molecules Nature 342 1989 154 158
11. S.H. Hutner, L. Provasoli, A. Schatz, and C.P. Haskins Some approaches to the study of the role of metals in the metabolism of microorganisms Proceedings of the American Philosophical Society 94 1950 152 170
12. J.S. Hyams, and G.G. Borisy Flagellar coordination in Chlamydomonas reinhardtii: Isolation and reactivation of the flagellar apparatus Science 189 1975 891 893
13. O. Kagami, and R. Kamiya Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein Journal of Cell Science 103 1992 653 664
14. R. Kamiya Analysis of cell vibration for assessing axonemal motility in Chlamydomonas Methods 22 2000 383 387
15. S.M. King, T. Otter, and G.B. Witman Purification and characterization of Chlamydomonas flagellar dyneins Methods in Enzymology 134 1986 291 306
16. S.J. Kron, and J.A. Spudich Fluorescent actin filaments move on myosin fixed to a glass surface Proceedings of the National Academy of Sciences 83 1986 6272 6276
17. C. Leduc, F. Ruhnow, J. Howard, and S. Diez Detection of fractional steps in cargo movement by the collective operation of kinesin-1 motors Proceedings of the National Academy of Sciences of the United States of America 104 2007 10847 10852
18. P.A. Lefebvre, S.A. Nordstrom, J.E. Moulder, and J.L. Rosenbaum Flagellar elongation and shortening in Chlamydomonas.4. Effects of flagellar detachment, regeneration, and resorption on induction of flagellar protein-synthesis The Journal of Cell Biology 78 1978 8 27
19. C.B. Lindemann, W.G. Rudd, and R. Rikmenspoel The stiffness of the flagella of impaled bull sperm Biophysical Journal 13 1973 437 448
20. I.H. Riedel-Kruse, A. Hilfinger, J. Howard, and F. Julicher How molecular motors shape the flagellar beat HFSP Journal 1 2007 192 208
21. D.L. Ringo Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas The Journal of Cell Biology 33 1967 543 571
22. F. Ruhnow, D. Zwicker, and S. Diez Tracking single particles and elongated filaments with nanometer precision Biophysical Journal 100 2011 2820 2828
23. R. Sager, and S. Granick Nutritional studies with Chlamydomonas reinhardtii Annals of the New York Academy of Sciences 56 1953 831 838
24. J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, and T. Pietzsch Fiji: An open-source platform for biological-image analysis Nature Methods 9 2012 676 682
25. K.E. Summers, and I.R. Gibbons Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm Proceedings of the National Academy of Sciences of the United States of America 68 1971 3092 3096
26. V. Varga, C. Leduc, V. Bormuth, S. Diez, and J. Howard Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization Cell 138 2009 1174 1183
27. P.O. Widlund, M. Podolski, S. Reber, J. Alper, M. Storch, and A.A. Hyman One-step purification of assembly competent tubulin from diverse eukaryotic sources Molecular Biology of the Cell 23 2012 4393 4401
28. G.B. Witman Isolation of Chlamydomonas flagella and flagellar axonemes Methods in Enzymology 134 1986 280 290
29. G.B. Witman, J. Plummer, and G. Sander Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components The Journal of Cell Biology 76 1978 729 747