Establishment of a triple co-culture in vitro cell models to study intestinal absorption of peptide drugs

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 83, Issue 3, 2013, Pages 427-435

  Antunes, Filipa ^a   Andrade, Fernanda ^a   Araújo, Francisca ^b   Ferreira, Domingos ^a   Sarmento, Bruno ^a,b,c  

^a UNIVERSITY OF PORTO   (Portugal)

^b UNIVERSITY INSTITUTE OF HEALTH SCIENCES IUCS   (Portugal)

^c INEB INSTITUTO DE ENGENHARIA BIOMÉDICA   (Portugal)

Author keywords

Caco 2 cells; Co culture; In vitro model; Intestinal absorption; Nanoparticles; Therapeutic proteins

Indexed keywords

INSULIN; NANOCARRIER; NANOPARTICLE; PEPTIDE;

ARTICLE; CELL MEMBRANE; CELL STRAIN CACO 2; CELL STRAIN HT29; CELL STRUCTURE; CELL VIABILITY; COCULTURE; CONTROLLED STUDY; FILTER; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; IN VITRO STUDY; INTESTINE ABSORPTION; INTESTINE EPITHELIUM; RAJI CELL; THICKNESS;

CACO-2 CELLS; COCULTURE TECHNIQUES; HT29 CELLS; HUMANS; INTESTINAL ABSORPTION; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; PEPTIDES; PERMEABILITY;

EID: 84875219051     PISSN: 09396411     EISSN: 18733441     Source Type: Journal    
DOI: 10.1016/j.ejpb.2012.10.003     Document Type: Article

References (43)

1. F. Antunes, F. Andrade, D. Ferreira, H.M. Nielsen, B. Sarmento, Models to predict intestinal absorption of therapeutic peptides and proteins, Curr. Drug Metab., in press.
2. P.V. Balimane, S. Chong, and R.A. Morrison Current methodologies used for evaluation of intestinal permeability and absorption J. Pharmacol. Toxicol. Methods 44 2000
3. K.M. Wood, G.M. Stone, and N.A. Peppas The effect of complexation hydrogels on insulin transport in intestinal epithelial cell models Acta Biomater. 6 2010 48 56
4. I.M.V.D. Lubben, F.A.C.V. Opdorp, M.R. Hengeveld, J.J.M. Onderwater, H.K. Koerten, J.C. Verhoef, G. Borchard, and H.E. Junginger Transport of chitosan microparticles for mucosal vaccine delivery in a human intestinal M-cell model J. Drug Target. 10 2002 449 456
5. P.V. Balimane, and S. Chong Cell culture-based models for intestinal permeability: a critique Drug Discov. Today 10 2005 335 343
6. M.L. Lind, J. Jacobsen, R. Holm, and A. Müllertz Development of simulated intestinal fluids containing nutrients as transport media in the Caco-2 cell culture model: assessment of cell viability, monolayer integrity and transport of a poorly aqueous soluble drug and a substrate of efflux mechanisms Eur. J. Pharm. Sci. 32 2007 261 270
7. C. Hilgendorf, H. Spahn-Langguth, C.G. Regardh, E. Lipka, G.L. Amidon, and P. Langguth Caco-2 versus Caco-2/HT29-MTX co-cultured cell lines: permeabilities via diffusion, inside- and outside-directed carrier-mediated transport J. Pharm. Sci. 89 2000 63 75
8. P. Anderle, E. Niederer, W. Rubas, C. Hilgendorf, H. Spahn-Langguth, H. Wunderli-Allenspach, H.P. Merkle, and P. Langguth P-glycoprotein (P-gp) mediated efflux in Caco-2 cell monolayers: the influence of culturing conditions and drug exposure on P-gp expression levels J. Pharm. Sci. 87 1998 757 762
9. X.-M. Chen, I. Elisia, and D.D. Kitts Defining conditions for the co-culture of Caco-2 and HT29-MTX cells using Taguchi design J. Pharmacol. Toxicol. Methods 2010
10. G.J. Mahler, M.L. Shuler, and R.P. Glahn Characterization of Caco-2 and HT29-MTX cocultures in an in vitro digestion/cell culture model used to predict iron bioavailability J. Nutr. Biochem. 20 2009 494 502
11. A. Wikman-Larhed, and P. Artursson Co-cultures of human intestinal goblet (HT29-H) and absorptive (Caco-2) cells for studies of drug and peptide absorption Eur. J. Pharm. Sci. 3 1995 171 183
12. G. Nollevaux, C. Deville, B. El Moualij, W. Zorzi, P. Deloyer, Y.J. Schneider, O. Peulen, and G. Dandrifosse Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) BMC Cell Biol. 7 2006 20
13. A.d. Rieux, E.G. Ragnarsson, E. Gullberg, V. Preat, Y.J. Schneider, and P. Artursson Transport of nanoparticles across an in vitro model of the human intestinal follicle associated epithelium Eur. J. Pharm. Sci. 25 2005 455 465
14. I. Kadiyala, Y. Loo, K. Roy, J. Rice, and K.W. Leong Transport of chitosan-DNA nanoparticles in human intestinal M-cell model versus normal intestinal enterocytes Eur. J. Pharm. Sci. 39 2010 103 109
15. Y.H. Lai, and M.J. D'Souza Microparticle transport in the human intestinal M cell model J. Drug Target. 16 2008 36 42
16. I.M.v.d. Lubben, F.A.v. Opdorp, M.R. Hengeveld, J.J. Onderwater, H.K. Koerten, J.C. Verhoef, G. Borchard, and H.E. Junginger Transport of chitosan microparticles for mucosal vaccine delivery in a human intestinal M-cell model J Drug Target. 10 2002 449 456
17. E. Gullberg, M. Leonard, J. Karlsson, A.M. Hopkins, D. Brayden, A.W. Baird, and P. Artursson Expression of specific markers and particle transport in a new human intestinal M-cell model Biochem. Biophys. Res. Commun. 279 2000 808 813
18. M.-K. Yoo, S.-K. Kang, J.-H. Choi, I.-K. Park, H.-S. Na, H.-C. Lee, E.-B. Kim, N.-K. Lee, J.-W. Nah, Y.-J. Choi, and C.-S. Cho Targeted delivery of chitosan nanoparticles to Peyer's patch using M cell-homing peptide selected by phage display technique Biomaterials 31 2010 7738 7747
19. T.C. Yih, and M. Al-Fandi Engineered nanoparticles as precise drug delivery systems J. Cell. Biochem. 97 2006 1184 1190
20. S. Shu, C. Sun, X. Zhang, Z. Wu, Z. Wang, and C. Li Hollow and degradable polyelectrolyte nanocapsules for protein drug delivery Acta Biomater. 6 2010 210 217
21. B. Sarmento, A. Ribeiro, F. Veiga, and D. Ferreira Development and characterization of new insulin containing polysaccharide nanoparticles Colloids Surf. B Biointerf. 53 2006 193 202
22. B. Sarmento, A. Ribeiro, F. Veiga, D. Ferreira, and R. Neufeld Oral bioavailability of insulin contained in polysaccharide nanoparticles Biomacromolecules 8 2007 3054 3060
23. A.d. Rieux, V. Fievez, I. Théate, J. Mast, V. Préat, and Y.-J. Schneider An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M cells Eur. J. Pharm. Sci. 30 2007 380 391
24. S. Kernéis, A. Bogdanova, J.-P. Kraehenbuhl, and E. Pringault Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria Science 277 1997 949 952
25. B. Sarmento, A. Ribeiro, F. Veiga, and D. Ferreira Development and validation of a rapid reversed-phase HPLC method for the determination of insulin from nanoparticulate systems Biomed Chromatogr 20 2006 898 903
26. P. Artursson, K. Palm, and K. Luthman Caco-2 monolayers in experimental and theoretical predictions of drug transport Adv. Drug Deliv. Rev. 46 2001 27 43
27. R. Bansil, and B.S. Turner Mucin structure, aggregation, physiological functions and biomedical applications Curr. Opin. Colloid Interf. Sci. 11 2006 164 170
28. P. Fonte, T. Nogueira, C. Gehm, D. Ferreira, and B. Sarmento Chitosan-coated solid lipid nanoparticles enhance the oral absorption of insulin Drug Deliv. Translation. Res. 1 2011 299 308
29. D.A. Carr, and N.A. Peppas Assessment of poly(methacrylic acid-co-N-vinyl pyrrolidone) as a carrier for the oral delivery of therapeutic proteins using Caco-2 and HT29-MTX cell lines J. Biomed. Mater. Res. Part A 92A 2010 504 512
30. E. Walter, S. Janich, B.J. Roessler, J.M. Hilfinger, and G.L. Amidon HT29-MTX/Caco-2 cocultures as an in vitro model for the intestinal epithelium: in vitro-in vivo correlation with permeability data from rats and humans J. Pharm. Sci. 85 1996 1070 1076
31. L.M. Ensign, R. Cone, and J. Hanes Oral drug delivery with polymeric nanoparticles: the gastrointestinal mucus barriers Adv. Drug Deliv. Rev. 64 2012 557 570
32. X.-M. Chen, I. Elisia, and D. Kitts Defining conditions for the co-culture of Caco-2 and HT29-MTX cells using Taguchi design J. Pharmacol. Toxicol. Methods 61 2010 334 342
33. Y.-H. Lin, F.-L. Mi, C.-T. Chen, W.-C. Chang, S.-F. Peng, H.-F. Liang, and H.-W. Sung Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery Biomacromolecules 8 2007 146 152
34. S.M.v.d. Merwe, J.C. Verhoef, J.H.M. Verheijden, A.F. Kotzé, and H.E. Junginger Trimethylated chitosan as polymeric absorption enhancer for improved peroral delivery of peptide drugs Eur J. Pharm. Biopharm. 58 2004 225 235
35. P.D. Ward, T.K. Tippin, and D.R. Thakker Enhancing paracellular permeability by modulating epithelial tight junctions Pharm. Sci. Technol. Today 3 2000 346 358
36. Y. Jin, Y. Song, X. Zhu, D. Zhou, C. Chen, Z. Zhang, and Y. Huang Goblet cell-targeting nanoparticles for oral insulin delivery and the influence of mucus on insulin transport Biomaterials 33 2012 1573 1582
37. V. Zoete, M. Meuwly, and M. Karplus A comparison of the dynamic behavior of monomeric and dimeric insulin shows structural rearrangements in the active monomer J. Mol. Biol. 342 2004 913 929
38. S. Kerneis, A. Bogdanova, J.-P. Kraehenbuhl, and E. Pringault Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria Science 277 1997 949 952
39. A. Bazes, G. Nollevaux, R. Coco, A. Joly, T. Sergent, and Y.-J. Schneider Development of a triculture based system for improved benefit/risk assessment in pharmacology and human food BMC Proc. 5 2011 P67
40. L. Yin, J. Ding, C. He, L. Cui, C. Tang, and C. Yin Drug permeability and mucoadhesion properties of thiolated trimethyl chitosan nanoparticles in oral insulin delivery Biomaterials 30 2009 5691 5700
41. C.B. Woitiski, B. Sarmento, R.A. Carvalho, R.J. Neufeld, and F. Veiga Facilitated nanoscale delivery of insulin across intestinal membrane models Int. J. Pharm. 412 2011 123 131
42. L. Yin, J. Ding, L. Fei, M. He, F. Cui, C. Tang, and C. Yin Beneficial properties for insulin absorption using superporous hydrogel containing interpenetrating polymer network as oral delivery vehicles Int. J. Pharm. 350 2008 220 229
43. S. Yamashita, Y. Tanaka, Y. Endoh, Y. Taki, T. Sakane, T. Nadai, and H. Sezaki Analysis of drug permeation across Caco-2 monolayer: implication for predicting in vivo drug absorption Pharm. Res. 14 1997 486 491