Generation of transgenic mice expressing EGFP protein fused to NP68 MHC class I epitope using lentivirus vectors

Genesis

Volumn 51, Issue 3, 2013, Pages 193-200

  Tomkowiak, Martine ^a,b   Ghittoni, Raffaella ^a,b   Teixeira, Marie ^b,c   Blanquier, Bariza ^b,c   Szécsi, Judit ^b,d,e   Nègre, Didier ^c,d,e   Aubert, Denise ^b,c   Coupet, Charles Antoine ^a,b   Brunner, Molly ^a,b   Verhoeyen, Els ^d,e   Thoumas, Jean Louis ^b,c   Cosset, François Loïc ^c,d,e   Leverrier, Yann ^a,b,c   Marvel, Jacqueline ^a,b,c  

^a INSERM   (France)

^b UNIVERSITÉ LYON 1   (France)

^c University Lyon i   (France)

^d CENTRE INTERNATIONAL DE RECHERCHE EN INFECTIOLOGIE   (France)

^e UNIVERSITÉ DE LYON   (France)

Author keywords

Antigen presentation; Cross presentation; Dendritic cells; T cell development; Thymus; Transgenesis

Indexed keywords

CD8ALPHA ANTIGEN; ENHANCED GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LENTIVIRUS VECTOR; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; NP68 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN EXPRESSION; ANTIGEN PRESENTATION; ARTICLE; CD8+ T LYMPHOCYTE; CELL DIFFERENTIATION; CONTROLLED STUDY; CROSS PRESENTATION; HEMATOPOIETIC STEM CELL; IN VITRO STUDY; MOUSE; NONHUMAN; PLASMACYTOID DENDRITIC CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SPLEEN FOCUS FORMING VIRUS; TRANSGENICS;

ANIMALS; DENDRITIC CELLS; EPITOPES, T-LYMPHOCYTE; GENETIC ENGINEERING; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HISTOCOMPATIBILITY ANTIGENS CLASS I; LENTIVIRUS; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; PROMOTER REGIONS, GENETIC; T-LYMPHOCYTES; TRANSGENES; VIRAL PROTEINS;

LENTIVIRUS; MUS MUSCULUS; SPLEEN FOCUS-FORMING VIRUS;

EID: 84875052240     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/dvg.22364     Document Type: Article

References (17)

1. Baup D, Moser M, Schurmans S, Leo O. 2009. Developmental regulation of the composite CAG promoter activity in the murine T lymphocyte cell lineage. Genesis 47:799-804.
2. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. 1993. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 90:8033-8037.
3. Cronin J, Zhang XY, Reiser J. 2005. Altering the tropism of lentiviral vectors through pseudotyping. Curr Gene Ther 5:387-398.
4. Dann CT. 2007. New technology for an old favorite: Lentiviral transgenesis and RNAi in rats. Transgenic Res 16:571-580.
5. de Brito C, Tomkowiak M, Ghittoni R, Caux C, Leverrier Y, Marvel J. 2011. CpG promotes cross-presentation of dead cell-associated antigens by pre-CD8alpha+ dendritic cells. J Immunol 186:1503-1511.
6. de Jersey J, Carmignac D, Barthlott T, Robinson I, Stockinger B. 2002. Activation of CD8 T cells by antigen expressed in the pituitary gland. J Immunol 169:6753-6759.
7. Dresch C, Leverrier Y, Marvel J, Shortman K. 2012. Development of antigen cross-presentation capacity in dendritic cells. Trends Immunol 33:381-388.
8. Frecha C, Toscano MG, Costa C, Saez-Lara MJ, Cosset FL, Verhoeyen E, Martin F. 2008. Improved lentiviral vectors for Wiskott-Aldrich syndrome gene therapy mimic endogenous expression profiles throughout haematopoiesis. Gene Ther 15:930-941.
9. Gonzalez-Murillo A, Lozano ML, Alvarez L, Jacome A, Almarza E, Navarro S, Segovia JC, Hanenberg H, Guenechea G, Bueren JA, Rio P. 2010. Development of lentiviral vectors with optimized transcriptional activity for the gene therapy of patients with Fanconi anemia. Hum Gene Ther 21:623-630.
10. Jubin V, Ventre E, Leverrier Y, Djebali S, Mayol K, Tomkowiak M, Mafille J, Teixeira M, Teoh DY, Lina B, Walzer T, Arpin C, Marvel J. 2012. T inflammatory memory CD8 T cells participate to antiviral response and generate secondary memory cells with an advantage in XCL1 production. Immunol Res 52:284-293.
11. Levelt CN, de Jong YP, Mizoguchi E, O'Farrelly C, Bhan AK, Tonegawa S, Terhorst C, Simpson SJ. 1999. High- and low-affinity single-peptide/MHC ligands have distinct effects on the development of mucosal CD8alphaalpha and CD8alphabeta T lymphocytes. Proc Natl Acad Sci USA 96:5628-5633.
12. Mamalaki C, Elliott J, Norton T, Yannoutsos N, Townsend AR, Chandler P, Simpson E, Kioussis D. 1993. Positive and negative selection in transgenic mice expressing a T-cell receptor specific for influenza nucleoprotein and endogenous superantigen. Dev Immunol 3:159-174.
13. Mamalaki C, Norton T, Tanaka Y, Townsend AR, Chandler P, Simpson E, Kioussis D. 1992. Thymic depletion and peripheral activation of class I major histocompatibility complex-restricted T cells by soluble peptide in T-cell receptor transgenic mice. Proc Natl Acad Sci USA 89:11342-11346.
14. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2003. Manipulating the mouse embryo: A laboratory manual, 3rd ed. New York: Cold Spring Harbor Laboratory Press. 764 p.
15. Park F. 2007. Lentiviral vectors: Are they the future of animal transgenesis? Physiol Genomics 31:159-173.
16. Van Maele B, De Rijck J, De Clercq E, Debyser Z. 2003. Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction. J Virol 77:4685-4694.
17. Zufferey R, Nagy D, Mandel RJ, Naldini L, Trono D. 1997. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat Biotechnol 15:871-875.