A Real-Time PCR Assay for the Detection of Atypical Strains of Chlamydiaceae from Pigeons

PLoS ONE

Volumn 8, Issue 3, 2013, Pages

  Zocevic, Aleksandar ^a   Vorimore, Fabien ^a   Vicari, Nadia ^b   Gasparini, Julien ^c   Jacquin, Lisa ^c   Sachse, Konrad ^d   Magnino, Simone ^b   Laroucau, Karine ^a  

^a UNIVERSITÉ PARIS EST   (France)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA LOMBARDIA E DELL EMILIA ROMAGNA   (Italy)

^c ECOLE NORMALE SUPÉRIEURE   (France)

^d FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; CHLAMYDIACEAE; CHLAMYDOPHILA PSITTACI; CONTROLLED STUDY; LIMIT OF DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; ORNITHOSIS; PIGEON; PROCESS DEVELOPMENT; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY;

ANIMALS; CHLAMYDIACEAE; COLUMBIDAE; DNA, BACTERIAL; GENOME, BACTERIAL; PHOSPHOPYRUVATE HYDRATASE; PHYLOGENY; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS;

EID: 84875016305     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0058741     Document Type: Article

References (18)

1. Greub G, (2010) International Committee on Systematics of Prokaryotes. Subcommittee on the taxonomy of the Chlamydiae: minutes of the closed meeting, 21 June 2010, Hof bei Salzburg, Austria. Int J Syst Evol Microbiol 60: 2694.
2. Andersen AA, (1997) Two new serovars of Chlamydia psittaci from North American birds. J Vet Diagn Invest 9: 159-164.
3. Deschuyffeleer TP, Tyberghien LF, Dickx VL, Geens T, Saelen JM, et al. (2012) Risk assessment and management of Chlamydia psittaci in poultry processing plants. Ann Occup Hyg 56(3): 340-9 Review.
4. Vanrompay D, Ducatelle R, Haesebrouck F, (1995) Chlamydia psittaci infections: a review with emphasis on avian chlamydiosis. Vet Microbiol 45: 93-119.
5. Herrmann B, Rahman R, Bergstrom S, Bonnedahl J, Olsen B, (2000) Chlamydophila abortus in a Brown skua (Catharacta antarctica lonnbergi) from a subantarctic island. Appl Environ Microbiol 66: 3654-3656.
6. Pantchev A, Sting R, Bauerfeind R, Tyczka J, Sachse K, (2009) New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. Vet J 181: 145-150.
7. Lemus JA, Fargallo JA, Vergara P, Parejo D, Banda E (2010) Natural cross chlamydial infection between livestock and free-living bird species. PLoS One 5e13512.
8. Sachse K, Kuehlewind S, Ruettger A, Schubert E, Rohde G, (2012) More than classical Chlamydia psittaci in urban pigeons. Vet Microbiol 157: 476-480.
9. Gaede W, Reckling KF, Dresenkamp B, Kenklies S, Schubert E, et al. (2008) Chlamydophila psittaci infections in humans during an outbreak of psittacosis from poultry in Germany. Zoonoses Public Health 55: 184-188.
10. Laroucau K, Vorimore F, Aaziz R, Berndt A, Schubert E, et al. (2009) Isolation of a new chlamydial agent from infected domestic poultry coincided with cases of atypical pneumonia among slaughterhouse workers in France. Infect Genet Evol 9: 1240-1247.
11. Robertson T, Bibby S, O'Rourke D, Belfiore T, Lambie H, et al. (2009) Characterization of Chlamydiaceae species using PCR and high resolution melt curve analysis of the 16S rRNA gene. J Applied Microbiol 107: 2017-2028.
12. Vicari N, Laroucau K, Vorimore F, Barbieri I, Sachse K, et al. (2009) Molecular analysis of four chlamydial isolates from the intestine and cloacal swabs of feral pigeons sampled in Milan and Ferrara, Italy. (Oral communication). 1st European Meeting on Animal Chlamydioses and Zoonotic Aspects.Murcia, Spain. 14-16/06/09.
13. Gasparini J, Erin N, Bertin C, Jacquin L, Vorimore F, et al. (2011) Impact of urban environment and host phenotype on the epidemiology of Chlamydiaceae in feral pigeons (Columba livia). Environ Microbiol 13: 3186-3193.
14. Zocevic A, Vorimore F, Marhold C, Horvatek D, Wang D, et al. (2012) Molecular characterization of atypical Chlamydia and evidence of their dissemination in different European and Asian chicken flocks by specific real-time PCR. Environ Microbiol 14(8): 2212-2222.
15. Pannekoek Y, Morelli G, Kusecek B, Morre SA, Ossewaarde JM, et al. (2008) Multi locus sequence typing of Chlamydiales: clonal groupings within the obligate intracellular bacteria Chlamydia trachomatis. BMC Microbiol 8: 42.
16. Pannekoek Y, Dickx V, Beeckman DS, Jolley KA, Keijzers WC, et al. (2010) Multi locus sequence typing of Chlamydia reveals an association between Chlamydia psittaci genotypes and host species. PLoS ONE 5: e14179.
17. Ehricht R, Slickers P, Goellner S, Hotzel H, Sachse K, (2006) Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies. Mol Cell Probes 20(1): 60-63.
18. Ménard A, Clerc M, Subtil A, Mégraud F, Bébéar C, et al. (2006) Development of a real-time PCR for the detection of Chlamydia psittaci. J Med Microbiol 55: 471-473.