Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering

Engineering in Life Sciences

Volumn 13, Issue 2, 2013, Pages 140-148

  Bice, Ismet ^a   Celik, Hilal ^a   Wolff, Christoph ^a   Beutel, Sascha ^a   Zahid, Maria ^a   Hitzmann, Bernd ^b   Rinas, Ursula ^a,c   Kasper, Cornelia ^d   Gerardy Schahn, Rita ^e   Scheper, Thomas ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b UNIVERSITY OF HOHENHEIM   (Germany)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^d UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^e HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Clay mineral; Escherichia coli K1; Membrane adsorber technique; Polysialic acid; Tissue engineering

Indexed keywords

CARBOHYDRATE POLYMERS; DEGREE OF POLYMERIZATION; DOWNSTREAM-PROCESSING; ETHANOL PRECIPITATION; FED-BATCH CULTIVATION; MEMBRANE ADSORBER; POLYSIALIC ACID; PURIFICATION PROCESS;

ACETONE; ADSORPTION; CLAY MINERALS; ESCHERICHIA COLI; LASERS; MEMBRANE TECHNOLOGY; POLYMERIZATION; PURIFICATION; SCAFFOLDS (BIOLOGY); TISSUE ENGINEERING;

CHAIN LENGTH;

ADSORBENT; ENDOTOXIN; GLUCOSE; MINERAL; POLYSIALIC ACID;

ADSORPTION; ANION; BIOENGINEERING; BIOREACTOR; CARBOHYDRATE; CLAY MINERAL; FECAL COLIFORM; INORGANIC ACID; ION EXCHANGE; MEMBRANE; POLYMER; POLYMERIZATION; PURIFICATION;

ADSORPTION; ARTICLE; BIOMASS; BIOREACTOR; CLAY; ESCHERICHIA COLI; FED BATCH CULTURE; MICROFILTRATION; NONHUMAN; OXIDATION; PH; POLYMERIZATION; PRECIPITATION; PROTEIN BINDING; PURIFICATION; STIRRED REACTOR; TISSUE ENGINEERING; ULTRAFILTRATION;

EID: 84875009796     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201200041     Document Type: Article

References (21)

1. Rodríguez-Aparicio, L. B., Reglero, A., Ortiz, A. I., Luengo, J. M., Effect of physical and chemical conditions on the production of colominic acid by Escherichia coli in a defined medium. Appl. Microbiol. Biotechnol. 1988, 27, 474-483.
2. Baumann, H., Brisson, J. R., Michon, F., Pon, R. et al., Comparison of the conformation of the epitope of alpha(2-8) polysialic acid with its reduced and N-acyl derivatives. Biochemistry 1993, 32, 4007-4013.
3. Angata, T., Varki, A., Chemical diversity in the sialic acids and related alpha-keto acids: an evolutionary perspective. Chem. Rev. 2002, 102, 439-469.
4. Gotschlich, E. C., Fraser, B. A., Nishimura, O., Robbins, J. B. et al., Lipid on capsular polysaccharides of gram-negative bacteria. J. Biol. Chem. 1981, 256, 8915-8921.
5. Tzeng, Y.-L., Datta, A. K., Strole, C. A., Lobritz, M. A. et al., Translocation and surface expression of lipidated serogroup B capsular polysaccharide in Neisseria meningitidis. Infect. Immun. 2005, 73, 1491-1505.
6. Whitfield, C., Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. Annu. Rev. Biochem. 2006, 75, 39-68.
7. Rode, B., Endres, C., Ran, C., Stahl, F. et al., Large-scale production and homogenous purification of long chain polysialic acids from E. coli K1. J. Biotechnol. 2008, 135, 202-209.
8. Liu, J.-L., Zhan, X.-B., Wu, J.-R., Lin, C.-C., Yu, D.-F., An efficient and large-scale preparation process for polysialic acid by Escherichia coli CCTCC M208088. Biochem. Eng. J. 2010, 53, 97-103.
9. Kapre, S. V., Shaligram, U., Highly pure polysialic acid and process for preperation thereof US Patent Application 20100086974, 2010.
10. Ralla, K., Sohling, U., Suck, K., Sander, F. et al., Adsorption and separation of proteins by a synthetic hydrotalcite. Colloids Surf. B 2011, 87, 217-225.
11. Kökpinar, O., Harkensee, D., Kasper, C., Scheper, T. et al., Innovative modular membrane adsorber system for high-throughput downstream screening for protein purification. Biotechnol. Prog. 22, 1215-1219.
12. Chen, R., John, J., Rode, B., Hitzmann, B. et al., Comparison of polysialic acid production in Escherichia coli K1 during batch cultivation and fed-batch cultivation applying two different control strategies. J. Biotechnol. 2011, 154, 222-229.
13. Graf, A.-M., Steinhof, R., Lotz, M., Tippkötter, N. et al., Downstream-Processing mit Membranadsorbern zur Isolierung nativer Proteinfraktionen aus Kartoffelfruchtwasser. Chem. Ing. Tech. 2009, 81, 267-274.
14. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
15. Inoue, S., Lin, S. L., Lee, Y. C., Inoue, Y., An ultrasensitive chemical method for polysialic acid analysis. Glycobiology 2001, 11, 759-767.
16. Nakata, D., Troy, F. A., Degree of polymerization (DP) of polysialic acid (polySia) on neural cell adhesion molecules (N-CAMS): development and application of a new strategy to accurately determine the DP of polySia chains on N-CAMS. J. Biol. Chem. 2005, 280, 38305-38316.
17. Suck, K., Walter, J., Menzel, F., Tappe, A., Kasper, C., Naumann, C., Zeidler, R., Scheper, T., Fast and efficient protein purification using membrane adsorber systems. J. Biotechnol. 2006, 121, 361-367.
18. Plate, K., Beutel, S., Buchholz, H., Demmer, W. et al., Isolation of bovine lactoferrin, lactoperoxidase and enzymatically prepared lactoferricin from proteolytic digestion of bovine lactoferrin using adsorptive membrane chromatography. J. Chromatogr. A 2006, 1117, 81-86.
19. Ralla, K., Anton, F., Scheper, T., Kasper, C., Application of conjoint liquid chromatography with monolithic disks for the simultaneous determination of immunoglobulin G and other proteins present in a cell culture medium. J. Chromatogr. A 2009, 1216, 2671-2675.
20. Ruiz-Hitzky, E., Darder, M., Aranda, P., Functional biopolymer nanocomposites based on layered solids. J. Mater. Chem. 2005, 15, 3650-3662.
21. Haastert-Talini, K., Schaper-Rinkel, J., Schmitte, R., Bastian, R. et al., In vivo evaluation of polysialic acid as part of tissue-engineered nerve transplants. Tissue Eng. A 2010, 16, 3085-3098.