Beclin1 inhibition promotes autophagy and decreases gemcitabine-induced apoptosis in Miapaca2 pancreatic cancer cells

Cancer Cell International

Volumn 13, Issue 1, 2013, Pages

  Li, Xiaoshu ^a,b   Yan, Jun ^c   Wang, Lisheng ^d   Xiao, Fengjun ^d   Yang, Yuefeng ^d   Guo, Xiaozhong ^a   Wang, Hua ^d  

^a GENERAL HOSPITAL OF NORTHERN THEATER COMMAND   (China)

^b Fourth People's Hospital of Shenyang   (China)

^c CHINESE PLA GENERAL HOSPITAL   (China)

^d BEIJING INSTITUTE OF RADIATION MEDICINE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BECLIN 1; GEMCITABINE; GREEN FLUORESCENT PROTEIN; MICROTUBULE ASSOCIATED PROTEIN 1; MICROTUBULE ASSOCIATED PROTEIN 1 LIGHT CHAIN 3 II; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; AUTOPHAGY; CANCER CELL; CELL CYCLE; CELL VIABILITY; CONTROLLED STUDY; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INHIBITION KINETICS; PANCREAS CANCER; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

EID: 84874807733     PISSN: None     EISSN: 14752867     Source Type: Journal    
DOI: 10.1186/1475-2867-13-26     Document Type: Article

References (20)

1. Mizushima N, Levine B, Cuervo AM, Klionsky DJ. Autophagy fights disease through cellular self-digestion. Nature 2008, 451(7182):1069-1075. 10.1038/nature06639, 2670399, 18305538.
2. Mathew R, Karantza-Wadsworth V, White E. Role of autophagy in cancer. Nat Rev Cancer 2007, 7(12):961-967. 10.1038/nrc2254, 2866167, 17972889.
3. Brech A, Ahlquist T, Lothe RA, Stenmark H. Autophagy in tumour suppression and promotion. Mol Oncol 2009, 3(4):366-375. 10.1016/j.molonc.2009.05.007, 19559660.
4. Levine B, Yuan J. Autophagy in cell death: an innocent convict?. Clin Invest 2005, 115(10):2679-2688.
5. Katayama M, Kawaguchi T, Berger MS, Pieper RO. DNA damaging agent-induced autophagy produces a cytoprotective adenosine triphosphate surge in malignant glioma cells. Cell Death Differ 2007, 14(3):548-558. 10.1038/sj.cdd.4402030, 16946731.
6. Baehrecke EH. Autophagy: dual roles in life and death?. Nat Rev Mol Cell Biol 2005, 6(6):505-510. 10.1038/nrm1666, 15928714.
7. Lum JJ, DeBerardinis RJ, Thompson CB. Autophagy in metazoans: cell survival in the land of plenty. Nat Rev Mol Cell Biol 2005, 6(6):439-448. 10.1038/nrm1660, 15928708.
8. Yorimitsu T, Klionsky DJ. Eating the endoplasmic reticulum: quality control by autophagy. Trends Cell Biol 2007, 17(6):279-285. 10.1016/j.tcb.2007.04.005, 17481899.
9. Aita VM, Liang XH, Murty VV, Pincus DL, Yu W, Cayanis E, Kalachikov S, Gilliam TC, Levine B. Cloning and genomic organization of beclin1, a candidate tumor suppressor gene on chromosome 17q21. Genomics 1999, 59(1):59-65. 10.1006/geno.1999.5851, 10395800.
10. Miracco C, Cosci E, Oliveri G, Luzi P, Pacenti L, Monciatti I, Mannucci S, De Nisi MC, Toscano M, Malagnino V, Falzarano SM, Pirtoli L, Tosi P. Protein and mRNA expression of autophagy gene Beclin 1 in human brain tumours. Int J Onco 2007, 30(2):429-436.
11. Mizushima N, Noda T, Yoshimori T, Tanaka Y, Ishii T, George MD, Klionsky DJ, Ohsumi M, Ohsumi Y. A protein conjugation system essential for autophagy. Nature 1998, 395(6700):395-398. 10.1038/26506, 9759731.
12. Ravikumar B, Imarisio S, Sarkar S, O'Kane CJ, Rubinsztein DC. Rab5 Modulates aggregation and toxicity of mutant huntingtin through macroautophagy in cell and fly models of Huntington disease. Cell Sci 2008, 121(Pt 10):1649-1660.
13. Liang XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature 1999, 402(6762):672-676. 10.1038/45257, 10604474.
14. Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y, Yoshimori T. LC3, A mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J 2000, 19(21):5720-5728. 10.1093/emboj/19.21.5720, 305793, 11060023.
15. Luo S, Rubinsztein DC. Apoptosis blocks beclin 1-dependent autophagosome synthesis: an effect rescued by Bcl-xL. Cell Death Differ 2010, 17(2):268-277. 10.1038/cdd.2009.121, 2894406, 19713971.
16. Qu X, Yu J, Bhagat G, Furuya N, Hibshoosh H, Troxel A, Rosen J, Eskelinen EL, Mizushima N, Ohsumi Y, Cattoretti G, Levine B. Promotion of tumorigenesis by heterozygous disruption of the beclin1 autophagy gene. Clin Invest 2003, 112(12):1809-1820.
17. Boya P, González-Polo RA, Casares N, Perfettini JL, Dessen P, Larochette N, Métivier D, Meley D, Souquere S, Yoshimori T, Pierron G, Codogno P, Kroemer G. Inhibition of macroautophagy triggers apoptosis. Mol Cell Biol 2005, 25(3):1025-1040. 10.1128/MCB.25.3.1025-1040.2005, 543994, 15657430.
18. Wang ZH, Peng ZL, Duan ZL, Liu H. Expression and clinical significance of autophagy gene Beclin1 in cervical squamous cell carcinoma. Sichuan Da Xue Xue Bao Yi Xue Ban 2006, 37(6):860-863.
19. Pattingre S, Tassa A, Qu X, Garuti R, Liang XH, Mizushima N, Packer M, Schneider MD, Levine B. Bcl-2 antiapoptotic proteins inhibit beclin 1-dependent autophagy. Cell 2005, 122(6):927-939. 10.1016/j.cell.2005.07.002, 16179260.
20. Saleem A, Dvorzhinski D, Santanam U, Mathew R, Bray K, Stein M, White E, DiPaola RS. Effect of dual inhibition of apoptosis and autophagy in prostate cancer. Prostate 2012, 72(12):1374-1381. 10.1002/pros.22487, 22241682.