Serial enrichment of spermatogonial stem and progenitor cells (SSCs) in culture for derivation of long-term adult mouse SSC lines

Journal of Visualized Experiments

Volumn , Issue 72, 2013, Pages

  Martin, Laura A ^a   Seandel, Marco ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

Anatomy; Animal model; Cell culture; Cellular biology; Developmental biology; Feeder cells; Genetics; Germ cells; Issue 72; Medicine; Mice; Microenvironment; Molecular biology; Progenitor cells; Spermatogonial stem cells; Stem cell biology; Stem cell niche; Stem cells; Surgery; Testis; Transgenic mice

Indexed keywords

EID: 84874526200     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50017     Document Type: Article

References (15)

1. Kanatsu-Shinohara, M., et al. Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol. Reprod. 69, 612-616 (2003).
2. Seandel, M., et al. Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature. 449, 346-350 (2007).
3. Kim, J., Seandel, M., Falciatori, I., Wen, D., & Rafii, S. CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells. Stem Cells. 26, 2516-2522. doi:10.1634/stemcells.2008-0379 (2008).
4. Ogawa, T., et al. Derivation and morphological characterization of mouse spermatogonial stem cell lines. Arch. Histol. Cytol. 67, 297-306 (2004).
5. Schmidt, J.A., et al. In vivo and in vitro aging is detrimental to mouse spermatogonial stem cell function. Biology of Reproduction. 84, 698-706. doi:10.1095/biolreprod.110.088229 (2011).
6. Zhang, X., Ebata, K. T., Robaire, B., & Nagano, M. C. Aging of male germ line stem cells in mice. Biology of Reproduction. 74, 119-124. doi:10.1095/biolreprod.105.045591 (2006).
7. Hobbs, R.M., Seandel, M., Falciatori, I., Rafii, S., & Pandolfi, P.P. Plzf regulates germline progenitor selfrenewal by opposing mTORC1. Cell. 142, 468-479 (2010).
8. Nagano, M., Ryu, B.Y., Brinster, C.J., Avarbock, M.R., & Brinster, R.L. Maintenance of mouse male germ line stem cells in vitro. Biol. Reprod. 68, 2207-2214 (2003).
9. Csaszar, E., et al. Rapid expansion of human hematopoietic stem cells by automated control of inhibitory feedback signaling. Cell Stem Cell. 10, 218-229. doi:10.1016/j.stem.2012.01.003 (2012).
10. Enders, G.C. & May, J.J. Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice. Dev. Biol. 163, 331-340 (1994).
11. Oatley, J.M. & Brinster, R.L. Regulation of spermatogonial stem cell self-renewal in mammals. Annu Rev Cell Dev Biol. 24, 263-286. doi:10.1146/annurev.cellbio.24.110707.175355 (2008).
12. Brinster, R.L. & Zimmermann, J.W. Spermatogenesis following male germ-cell transplantation. Proc Natl Acad Sci U.S.A. 91, 11298-11302 (1994).
13. Tang, L., Rodriguez-Sosa, J.R., & Dobrinski, I. Germ Cell Transplantation and Testis Tissue Xenografting in Mice. J. Vis. Exp. (60), e3545. doi:10.3791/3545 (2012).
14. Arnold, K., et al. Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice. Cell Stem Cell. 9, 317-329. doi:10.1016/j.stem.2011.09.001 (2011).
15. Yeh, J. R., Zhang, X., & Nagano, M. C. Establishment of a short-term in vitro assay for mouse spermatogonial stem cells. Biology of Reproduction. 77, 897-904. doi:10.1095/biolreprod.107.063057 (2007).