Very high throughput electrical cell lysis and extraction of intracellular compounds using 3D carbon electrodes in Lab-On-A-Chip devices

Micromachines

Volumn 3, Issue 3, 2012, Pages 574-581

  Mernier, Guillaume ^a   Martinez Duarte, Rodrigo ^a   Lehal, Rajwinder ^a   Radtke, Freddy ^a   Renaud, Philippe ^a  

^a EPFL   (Switzerland)

Author keywords

Carbon electrodes; Cell lysis; Microfluidics

Indexed keywords

BULK CHEMICALS; CARBON ELECTRODE; CELL LYSIS; ELECTRICAL CELLS; HIGH CELL DENSITY; HIGH THROUGHPUT; LAB-ON-A-CHIP DEVICES; MAMMALIAN CELLS; MICROLITERS; MINIATURIZED DEVICES; OFF-CHIP; SAMPLE PREPARATION; STAND-ALONE DEVICES; YEAST CELL;

EXTRACTION; LAB-ON-A-CHIP; MICROFLUIDICS;

ELECTRODES;

EID: 84874501440     PISSN: None     EISSN: 2072666X     Source Type: Journal    
DOI: 10.3390/mi3030574     Document Type: Article

References (20)

1. Zimmermann, U.; Pilwat, G.; Holzapfel, C.; Rosenheck, K. Electrical hemolysis of human and bovine red blood cells. J. Membr. Biol. 1976, 30, 135-152.
2. Salton, M.R.J. Cell structure and the enzymic lysis of bacteria. J. Gen. Microbiol. 1953, 9, 512-523.
3. Bramley, T.A.; Colemana, R.; Fineana, J.B. Chemical, enzymological and permeability properties of human erythrocyte ghosts prepared by hypotonic lysis in media of different osmolarities. Biochim. Biophys. Acta Biomembr. 1971, 241, 752-769.
4. Irimia, D.; Tompkins, R.G.; Toner, M. Single-cell chemical lysis in picoliter-scale closed volumes using a microfabricated device. Anal. Chem. 2004, 76, 6137-6143.
5. Jeltsch, E.; Zimmermann, U. Particles in a homogeneous electrical field: A model for the electrical breakdown of living cells in a coulter counter. J. Electroanal. Chem. 1979, 104, 349-384.
6. Han, F.; Wang, Y.; Sims, C.E.; Bachman, M.; Chang, R.; Li, G.P.; Allbritton, N.L. Fast electrical lysis of cells for capillary electrophoresis. Anal. Chem. 2003, 75, 3688-3696.
7. Pethica, B.A. Lysis by physical and chemical methods. J. Gen. Microbiol. 1958, 18, 473-480.
8. Di Carlo, D.; Jeong, K.H.; Lee, L.P. Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation. Lab Chip 2003, 3, 287-291.
9. Rau, K.R.; Guerra, A.; Vogel, A. Investigation of laser-induced cell lysis using time-resolved imaging. Appl. Phys. Lett. 2004, 84, 2940-2942.
10. Dhawan, M.D.; Wise, F.; Baeumner, A.J. Development of a laser-induced cell lysis system. Anal. Bional. Chem. 2002, 374, 421-426.
11. Wang, H.-Y.; Bhunia, A.K.; Lu, C. A microfluidic flow-through device for high throughput electrical lysis of bacterial cells based on continuous dc voltage. Biosens. Bioelectron. 2006, 22, 582-588.
12. Lu, H.; Schmidt, M.A.; Jensen, K.F. A microfluidic electroporation device for cell lysis. Lab Chip 2005, 5, 23-29.
13. Mernier, G.; Piacentini, N.; Renaud, P. Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting. Lab Chip 2010, 10, 2077-2082.
14. Mernier, G.; Hasenkamp, W.; Piacentini, N.; Renaud, P. Multiple-frequency impedance measurements in continuous flow for automated evaluation of yeast cell lysis. Sens Actuators B 2010, doi:10.1016/j.snb.2010.10.050.
15. Lee, D.W.; Cho, Y.H. A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture. Sens. Actuators B 2007, 124, 84-89.
16. Mernier, G.; Piacentini, N.; Renaud, P. Multiple-frequency impedance measurements in continuous flow for the evaluation of electrical lysis of yeast cells. Procedia Eng. 2010, 5, 37-40.
17. Shahini, M.; Yeow, J.T.W. Carbon nanotubes for voltage reduction and throughput enhancement of electrical cell lysis on a lab-on-a-chip. Nanotechnology 2011, 22, doi:10.1088/0957-4484/22/32/325705.
18. Martinez-Duarte, R.; Renaud, P.; Madou, M. A novel approach to Dielectrophoresis using carbon electrodes. Electrophoresis 2011, 32, 2385-2392.
19. Martinez-Duarte, R.; Gorkin, R.; Abi-Samra, K.; Madou, M. The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform. Lab Chip 2010, 10, 1030-1043.
20. Müller, K.J.; Sukhorukov, V.L.; Zimmermann, U. Intracellular delivery of trehalose into mammalian cells by electropermeabilization. J. Membr. Biol. 2001, 184, 161-170.