Construction and identification of recombinant adenovirus vector for expression of shRNA targeting phospholipase C epsilon gene

Chinese Journal of Biologicals

Volumn 26, Issue 1, 2013, Pages 51-55

  Zhang, Yan Yi ^a   Ou, Li Ping ^b   Liu, Qi ^b   Tao, Jia ^b   Wu, Xiao Hou ^b   Luo, Chun Li ^b  

^a CHONGQING MEDICAL UNIVERSITY   (China)

^b NONE

Author keywords

Adenovirus; Bladder cancer; PLC gene; RNA interference

Indexed keywords

ADENOVIRUS VECTOR; PHOSPHOLIPASE C; PHOSPHOLIPASE C EPSILON; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ADENOVIRUS; ARTICLE; GENE EXPRESSION; GENE TARGETING; NONHUMAN; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

EID: 84874201411     PISSN: 10045503     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Song C, Hu CD, Masago M, et al. Regulation of a novel human phospholipase C, PLCepsilon, through membrane targeting by Ras[J]. J Biol Chem, 2001, 276(4): 2752-2757.
2. Wing MR, Bourdon DM, Harden TK, et al. PLC-epsilon: a shared effector protein in Ras-, Rho-, and G alpha beta gamma-mediated signaling [J]. Mol Interv, 2004, 3(5): 273-280.
3. Oka M, Edamatsu H, Kunisada M, et al. Phospholipase Cε has a crucial role in ultraviolet B-induced neutrophil-associated skin inflammation by regulating the expression of CXCL-1 / KC [J]. Lab Invest, 2011, 91(5): 711-718.
4. Seifert JP, Zhou Y, Hicks SN, et al. Dual activation of phospholipase C-ε by Rho and Ras GTPases[J]. J Biol Chem, 2008, 283(44): 29690-29698.
5. Bunney TD, Baxendale RW, Katan M. Regulatory links between PLC enzymes and Ras superfamily GTPases: sig-nalling via PL-Cepsilon [J]. Adv Enzyme Reg-ul, 2009, 49(1): 54-58.
6. 2+- controlled activation of Ras and extracellular signal-regulated kinases by Gs-coupled receptors [J]. J Biol Chem, 2004, 279(45): 46497-46508.
7. Ikuta S, Edamatsu H, Li M, et al. Crucial role of phospholipase C epsilon in skin inflammation induced by tumor-promoting phorbolester [J]. Cancer Res, 2008, 68(1): 64-72. (Pubitemid 351380106)
8. Li M, Edamatsu H, Kitazawa R, et al. Phospholipase Cepsilon promotes intestinal tumorigenesis of Apc(Min / +) mice through augmentation of inflammation and angiogen-esis [J]. Carcinogenesis, 2009, 30(8): 1424-1432.
9. 2+ signaling, and cy-toskeleton modification in head and neck squamous cell carcinoma cells [J]. J Biol Chem, 2006, 281 (20): 14026-14040.
10. Yun S, Hong WP, Choi JH, et al. Phospholipase C-ε augments epidermal growth factor-dependent cell growth by inhibiting epidermal growth factor receptor down-regulation [J]. J Biol Chem, 2008, 283(1): 341-349.
11. Rao DD, Vorhies JS, Senzer N, et al. siRNA vs. shRNA: similarities and differences [J]. Adv Drug Deliv Rev, 2009, 61(9): 746-759.