The MTT assay is a rapid and reliable quantitative method to assess Staphylococcus aureus induced endothelial cell damage

Journal of Microbiological Methods

Volumn 92, Issue 3, 2013, Pages 307-309

  Seidl, Kati ^a   Zinkernagel, Annelies S ^a  

^a UNIVERSITY HOSPITAL ZURICH   (Switzerland)

Author keywords

A549; Damage; Ea.hy926; HUVECs; MTT assay; Staphylococcus aureus

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; CHROMIUM 51;

ARTICLE; BACTERIAL STRAIN; BIOASSAY; CELL DAMAGE; CHROMIUM 51 RELEASE; CONTROLLED STUDY; HUMAN; HUMAN CELL; LIMIT OF DETECTION; METABOLIC ACTIVATION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RELIABILITY; SCREENING TEST; STAPHYLOCOCCUS AUREUS; UMBILICAL VEIN ENDOTHELIAL CELL;

CELL SURVIVAL; CELLS, CULTURED; CHROMIUM RADIOISOTOPES; CYTOLOGICAL TECHNIQUES; ENDOTHELIAL CELLS; HUMANS; STAINING AND LABELING; STAPHYLOCOCCUS AUREUS; TETRAZOLIUM SALTS; THIAZOLES;

EID: 84873744295     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.12.018     Document Type: Article

References (20)

1. Balwit J., van Langevelde P., Vann J., Proctor R. Gentamicin-resistant menadione and hemin auxotrophic Staphylococcus aureus persist within cultured endothelial cells. J. Infect. Dis. 1994, 170(4):1033-1037.
2. Berridge M.V., Tan A.S. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys. 1993, 303(2):474-482.
3. Cheung A.L., Bayles K.W. Tissue culture assays used to analyze invasion by Staphylococcus aureus. Curr. Protoc. Microbiol. Suppl. 2007, (4):1-7. (Chapter 9, Unit 9C).
4. Edgell C.J., McDonald C.C., Graham J.B. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. U. S. A. 1983, 80(12):3734-3737.
5. Edwards A.M., Potts J.R., Josefsson E., Massey R.C. Staphylococcus aureus host cell invasion and virulence in sepsis is facilitated by the multiple repeats within FnBPA. PLoS Pathog. 2010, 6(6):e1000964.
6. Garrard W., Lascelles J. Regulation of Staphylococcus aureus lactate dehydrogenase. J. Bacteriol. 1968, 95(1):152-156.
7. Hansen M.B., Nielsen S.E., Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods 1989, 119(2):203-210.
8. Haslinger-Löffler B., Kahl B.C., Grundmeier M., Strangfeld K., Wagner B., Fischer U., Cheung A.L., Peters G., Schulze-Osthoff K., Sinha B. Multiple virulence factors are required for Staphylococcus aureus-induced apoptosis in endothelial cells. Cell. Microbiol. 2005, 7(8):1087-1097.
9. Kubica M., Guzik K., Koziel J., Zarebski M., Richter W., Gajkowska B., Golda A., Maciag-Gudowska A., Brix K., Shaw L., Foster T., Potempa J. A potential new pathway for Staphylococcus aureus dissemination: the silent survival of S. aureus phagocytosed by human monocyte-derived macrophages. PLoS One 2008, 3(1):e1409.
10. Lam T.-T., Giese B., Chikkaballi D., Kuhn A., Wolber W., Pane-Farre J., Schafer D., Engelmann S., Fraunholz M., Sinha B. Phagolysosomal integrity is generally maintained after Staphylococcus aureus invasion of nonprofessional phagocytes but Is modulated by strain 6850. Infect. Immun. 2010, 78(8):3392-3403.
11. Lemichez E., Lecuit M., Nassif X., Bourdoulous S. Breaking the wall: targeting of the endothelium by pathogenic bacteria. Nat. Rev. Microbiol. 2010, 8(2):93-104.
12. Lieber M., Smith B., Szakal A., Nelson-Rees W., Todaro G. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int. J. Cancer 1976, 17(1):62-70.
13. Scheglovitova O., Scanio V., Fais S., Papadia S., Abbate I., Castilletti C., Dianzani F., Capobianchi M. Antibody to ICAM-1 mediates enhancement of HIV-1 infection of human endothelial cells. Arch. Virol. 1995, 140(5):951-958.
14. Schnaith A., Kashkar H., Leggio S.A., Addicks K., Krönke M., Krut O. Staphylococcus aureus subvert autophagy for induction of caspase-independent host cell death. J. Biol. Chem. 2007, 282(4):2695-2706.
15. Seidl K., Bayer A.S., McKinnell J.A., Ellison S., Filler S.G., Xiong Y.Q. In vitro endothelial cell damage is positively correlated with enhanced virulence and poor vancomycin responsiveness in experimental endocarditis due to methicillin resistant Staphylococcus aureus. Cell. Microbiol. 2011, 13(10):1530-1541.
16. Seidl K., Solis N.V., Bayer A.S., Hady W.A., Ellison S., Klashman M.C., Xiong Y.Q., Filler S.G. Divergent responses of different endothelial cell types to infection with Candida albicans and Staphylococcus aureus. PLoS One 2012, 7(6):e39633.
17. Tuchscherr L., Heitmann V., Hussain M., Viemann D., Roth J., von Eiff C., Peters G., Becker K., Loeffler B. Staphylococcus aureus small-colony variants are adapted phenotypes for intracellular persistence. J. Infect. Dis. 2010, 202(7):1031-1040.
18. Vann J.M., Proctor R.A. Ingestion of Staphylococcus aureus by bovine endothelial cells results in time- and inoculum-dependent damage to endothelial cell monolayers. Infect. Immun. 1987, 55(9):2155-2163.
19. Walencka E., Sadowska B., Róžalska S., Hryniewicz W., Róžalska B. Staphylococcus aureus biofilm as a target for single or repeated doses of oxacillin, vancomycin, linezolid and/or lysostaphin. Folia Microbiol. 2006, 51(5):381-386.
20. Wisplinghoff H., Bischoff T., Tallent S.M., Seifert H., Wenzel R.P., Edmond M.B. Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin. Infect. Dis. 2004, 39(3):309-317.