Label-free segmentation of co-cultured cells on a nanotopographical gradient

Nano Letters

Volumn 13, Issue 2, 2013, Pages 570-576

  Reynolds, Paul M ^a   Pedersen, Rasmus H ^a   Stormonth Darling, John ^a   Dalby, Matthew J ^b   Riehle, Mathis O ^b   Gadegaard, Nikolaj ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

co culture; high throughput screening; label free; machine learning; Nanotopography

Indexed keywords

AUTOMATED DETECTION; CELL RESPONSE; COCULTURE; CULTURE SUBSTRATE; DESCRIPTORS; HIGH-THROUGHPUT SCREENING; IMPLANT DEVICES; INDIVIDUAL CELLS; LABEL FREE; LARGE IMAGES; NANO-PILLAR ARRAYS; NANO-STRUCTURING; NANOTOPOGRAPHIES; SET OF RULES; SINGLE CELLS; SURFACE PARAMETER; SYSTEMATIC STUDY; TOPOGRAPHICAL VARIATIONS; VASCULAR STENTS;

BODY FLUIDS; CELLS; CYTOLOGY; LEARNING SYSTEMS; NANOTECHNOLOGY;

IMAGE SEGMENTATION;

NANOMATERIAL;

ARTICLE; CELL TRACKING; CHEMISTRY; COCULTURE; CYTOLOGY; ENDOTHELIUM CELL; EQUIPMENT; FIBROBLAST; FLUORESCENCE; HUMAN; METHODOLOGY; NANOTECHNOLOGY;

CELL TRACKING; COCULTURE TECHNIQUES; ENDOTHELIAL CELLS; FIBROBLASTS; FLUORESCENCE; HUMANS; NANOSTRUCTURES; NANOTECHNOLOGY;

MLCS; MLOWN;

EID: 84873695094     PISSN: 15306984     EISSN: 15306992     Source Type: Journal    
DOI: 10.1021/nl304097p     Document Type: Article

References (37)

1. Alberts, B.; Johnson, A.; Lewis, J.; Raff, M.; Roberts, K.; Walter, P. Mol. Biol. Cell 2008, 1725
2. Vickaryous, M. K.; Hall, B. K. Biol. Rev. Cambridge Philos. Soc. 2006, 81, 425-55
3. Anderson, D. G.; Levenberg, S.; Langer, R. Nat. Biotechnol. 2004, 22, 863-6
4. Unadkat, H. V; Hulsman, M.; Cornelissen, K.; Papenburg, B. J.; Truckenmüller, R. K.; Post, G. F.; Uetz, M.; Reinders, M. J. T.; Stamatialis, D.; Van Blitterswijk, C. a; De Boer, J. Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 16565-70
5. Geysen, H. M.; Schoenen, F.; Wagner, D.; Wagner, R. Nat. Rev. Drug Discovery 2003, 2, 222-30
6. Kirkpatrick, C. J.; Fuchs, S.; Unger, R. E. Adv. Drug Delivery Rev. 2011, 63, 291-9
7. Khetani, S. R.; Bhatia, S. N. Nat. Biotechnol. 2008, 26, 120-6
8. Ngo, J. T.; Champion, J. a; Mahdavi, A.; Tanrikulu, I. C.; Beatty, K. E.; Connor, R. E.; Yoo, T. H.; Dieterich, D. C.; Schuman, E. M.; Tirrell, D. a Nat. Chem. Biol. 2009, 5, 715-7
9. Dalby, M. J.; Gadegaard, N.; Tare, R.; Andar, A.; Riehle, M. O.; Herzyk, P.; Wilkinson, C. D. W.; Oreffo, R. O. C. Nat. Mater. 2007, 6, 997-1003
10. Rivera Gil, P.; Yang, F.; Thomas, H.; Li, L.; Terfort, A.; Parak, W. J. Nano Today 2011, 6, 20-27
11. Kamentsky, L.; Jones, T. R.; Fraser, A.; Bray, M.-A.; Logan, D. J.; Madden, K. L.; Ljosa, V.; Rueden, C.; Eliceiri, K. W.; Carpenter, A. E. Bioinformatics 2011, 27, 1179-80
12. Jones, T. R.; Carpenter, A. E.; Lamprecht, M. R.; Moffat, J.; Silver, S. J.; Grenier, J. K.; Castoreno, A. B.; Eggert, U. S.; Root, D. E.; Golland, P.; Sabatini, D. M. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 1826-31
13. Cardona, A.; Tomancak, P. Nat. Methods 2012, 9, 661-5
14. Cho, B. H.; Cao-Berg, I.; Bakal, J. A.; Murphy, R. F. Nat. Methods 2012, 9, 633-4
15. Jones, T. R.; Carpenter, A. E.; Lamprecht, M. R.; Moffat, J.; Silver, S. J.; Grenier, J. K.; Castoreno, A. B.; Eggert, U. S.; Root, D. E.; Golland, P.; Sabatini, D. M. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 1826-31
16. Anderson, D. G.; Putnam, D.; Lavik, E. B.; Mahmood, T. a; Langer, R. Biomaterials 2005, 26, 4892-7
17. Kolind, K.; Dolatshahi-Pirouz, A.; Lovmand, J.; Pedersen, F. S.; Foss, M.; Besenbacher, F. Biomaterials 2010, 31, 9182-91
18. Kunzler, T. P.; Drobek, T.; Schuler, M.; Spencer, N. D. Biomaterials 2007, 28, 2175-82
19. Gallant, N. D.; Lavery, K. a.; Amis, E. J.; Becker, M. L. Adv. Mater. 2007, 19, 965-969
20. Zelzer, M.; Majani, R.; Bradley, J. W.; Rose, F. R. a J.; Davies, M. C.; Alexander, M. R. Biomaterials 2008, 29, 172-84
21. Simon, C. G.; Lin-Gibson, S. Adv. Mater. 2011, 23, 369-87
22. Glassford, S.; Chan, K. L. A.; Byrne, B.; Kazarian, S. G. Langmuir 2012, 28, 3174-9
23. Ding, Y.; Sun, J.; Ro, H. W.; Wang, Z.; Zhou, J.; Lin, N. J.; Cicerone, M. T.; Soles, C. L.; Lin-Gibson, S. Adv. Mater. 2011, 23, 421-5
24. Yang, J.; Rose, F. R. a. J.; Gadegaard, N.; Alexander, M. R. Adv. Mater. 2009, 21, 300-304
25. De Mel, A.; Jell, G.; Stevens, M. M.; Seifalian, A. M. Biomacromolecules 2008, 9, 2969-79
26. Bakker, R. M.; Boltasseva, A.; Liu, Z.; Pedersen, R. H.; Gresillon, S.; Kildishev, A. V; Drachev, V. P.; Shalaev, V. M. Opt. Express 2007, 15, 13682-13688
27. Gadegaard, N.; Mosler, S.; Larsen, N. B. Macromol. Mater. Eng. 2003, 288, 76-83
28. Schift, H.; David, C.; Gobrecht, J.; D' Amore, a.; Simoneta, D.; Kaiser, W.; Gabriel, M. J. Vac. Sci. Technol., B 2000, 18, 3564
29. Stormonth-Darling, J. M.; Gadegaard, N. Macromol. Mater. Eng. 2012, 297, 1075-1080
30. Curtis, A. S. G. Eur. Cell Mater. 2004, 8, 27-36
31. Csaderova, L.; Martines, E.; Seunarine, K.; Gadegaard, N.; Wilkinson, C. D. W.; Riehle, M. O. Small 2010, 6, 2755-2761
32. Dalby, M. J.; Giannaras, D.; Riehle, M. O.; Gadegaard, N.; Affrossman, S.; Curtis, A. S. G. Biomaterials 2004, 25, 77-83
33. Gadegaard, N.; Thoms, S.; Macintyre, D. S.; Mcghee, K.; Gallagher, J.; Casey, B.; Wilkinson, C. D. W. Micro 2003, 68, 162-168
34. Pedersen, R. H.; Hamzah, M.; Thoms, S.; Roach, P.; Alexander, M. R.; Gadegaard, N. Microelectron. Eng. 2010, 87, 1112-1114
35. Reynolds, P. M.; Pedersen, R. H.; Riehle, M. O.; Gadegaard, N. Small 2012, 8, 2541-2547
36. Boss, J. Exp. Cell Res. 1954, 7, 215-231
37. Friedman, J.; Hastie, T.; Tibshirani, R.; Friedman, B. Y. J.; Hastie, T. (2000, 28, 337-374.