Spectrophotometric Assays of Antioxidants

Oxidative Stress in Aquatic Ecosystems

Volumn , Issue , 2011, Pages 367-380

  Regoli, Francesco ^a   Bocchetti, Raffaella ^a   Filho, Danilo Wilhelm ^b  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^b FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

Author keywords

CAT, heme containing enzyme in peroxisomes; Glutathione (gsh) content; GST, family of multifunctional enzymes; Homogenization reagents and solutions; Less common units, expressing cat activity; Normalizing gsh concentration to tissue weight; Sample storage and preparation; Spectrophotometric assays of antioxidants; Superoxide dismutases activity; Technical protocols, measuring common antioxidants

Indexed keywords

EID: 84873454724     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781444345988.ch27     Document Type: Chapter

References (27)

1. Aebi, H. (1984) Catalase in vitro. Methods in Enzymolology 105, 121-126.
2. Akerboom, T.P.M., Sies, H. (1981) Assay of glutathione, glutathione disulfide and glutathione mixed disulfides in biological samples. Methods in Enzymology 71, 373-382.
3. Bergmeyer, H.U. (ed.) (1965) Methods of Enzymatic Analysis. Academic Press, New York, pp. 885-894.
4. Chance, B., Boveris, A., Oshino, N., Loschen, G. (1973) The nature of the catalase intermediate in the biological function. In King, T., Mason, H., Morrison, M. (eds). Oxidases and Related Redox Systems, Vol. I. University Park Press, Baltimore.
5. De Almeida, E.A., Bainy, A.C.D., de Melo Loureiro, A.P. et al. (2007) Oxidative stress in Perna perna and other bivalves as indicators of environmental stress in the Brazilian marine environment: antioxidants, lipid peroxidation and DNA damage. Comparative Biochemistry and Physiology A 146, 588-600.
6. Fernandez, C., San Miguel, E., Fernandez-Briera, A. (2009) Superoxide dismutase and catalase: tissue activities and relation with age in the long-lived species Margaritifera margaritifera. Biological Research 42, 57-68.
7. Fridovich, I. (1986) Superoxide dismutases. Advances in Enzymology and Related Areas ofMolecular Biology 58, 61-97.
8. George, S.G. (1994) Enzymology and molecular biology of phase II xenobiotic conjugation enzymes in fish. In Malins, D.C., Ostrander, G.K. (eds). Molecular Biological Approaches to Aquatic Toxicology. Lewis Publisher, Boca Raton, FL, pp. 37-85.
9. Habig, W.H., Jacoby, W.B. (1981) Assays for differentiation of glutathione S-transferases. Methods in Enzymology 77, 398-405.
10. Halliwell, B., Aruoma, O.I. (1991) DNA damage by oxygen derived species. Its mechanism and measurement in mammalian system. FEBS Letters 281, 9-19.
11. Halliwell, B., Gutteridge, J.M.C. (2001) Free Radicals in Biology and Medicine. University Press, Oxford, UK, pp. 936.
12. Halliwell, B., Gutteridge, J.M.C. (2007) Free Radicals in Biology and Medicine, 4rd edn. Oxford University Press, New York.
13. Lawrence, R.A., Burk, R.F. (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and Biophysical Research Communications 71, 952-958.
14. Mannervik, B.I., Carlberg, I., Larson, K. (1989) Glutathione. General review of mechanisms of action. In Dolphin, D., Poulsen, R., Wiley A.O. (eds) Glutathione: Chemical, Biochemical and Medical aspects, Part A. John Wiley & Sons, New York, pp. 475-516.
15. McCord, J.M., Fridovich, I. (1976) Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry 244, 6049-6055.
16. Meister, A. (1989) On the biochemistry of glutathione. In Taniruchi, N., Higashi, T., Sakamoto, S., Meyster, A. (eds) Glutathione Centennial: Molecular Prospectives and Clinical implications. Academic Press, San Diego, CA, pp. 3-22.
17. Meister, A., Anderson, M.E. (1983). Glutathione. Annual Review of Biochemistry 52, 711-760.
18. 2-removing enzyme in human erythrocytes. Blood 90, 4973-4978.
19. Principato, G.B., Locci, P., Rosi, G. et al. (1983) Activity changes of glyoxalase I-II and glutathione reductase in regenerating rat liver. Biochemical International 6, 249-255.
20. Principato, G.B., Rosi, G., Talesa, V. et al. (1987) Purification and characterization of two forms of glyoxalase II from the liver and brain of Wistar rats. Biochimica et Biophysica Acta 911, 349-355.
21. Regoli, F. (1992) Lysosomal responses as a sensitive stress index in biomonitoring heavy metal pollution. Marine Ecology Progress Series 84, 63-69.
22. Regoli, F., Principato, G.B. (1995) Glutathione, glutathionedependent and antioxidant enzymes in mussels, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of chemical biomarkers. Aquatic Toxicology 31, 143-164.
23. Regoli, F., Pellegrini, D., Winston, G.W. et al. (2002) Application of biomarkers for assessing the biological impact of dredged materials in the Mediterranean: The relationship between antioxidant responses and susceptibility to oxidative stress in the red mullet (Mullus barbatus). Marine Pollution Bulletin 44, 912-922.
24. Romero-Ruiz, A., Amezcua, O., Rodriíguez-Ortega, M.J., Muñoz, J.L. et al. (2003) Oxidative stress biomarkers in bivalves transplanted to the Guadalquivir estuary after Aznalcollar spill. Environmental Toxicology and Chemistry 22, 92-100.
25. Viarengo, A. (1989) Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular levels. Critical Review in Aquatic Science 1, 295-317.
26. Viarengo, A., Canesi, L., Pertica,M. et al. (1990) Heavymetals effects on lipid peroxidation in the tissues of Mytilus galloprovincialis Lam. Comparative Biochemistry and PhysiologyC 97, 37-42.
27. Vlahogianni, T., Dassenakis, M., Scoullos, M.J., Valavanidis, A. (2007) Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussel Mytilus galloprovincialis for assessing heavy metals' pollution in coastal areas from the Saronikos Gulf of Greece. Marine Pollution Bulletin 54, 1361-1371.