Loading fluorescent Ca2+ indicators into living cells

Cold Spring Harbor Protocols

Volumn 8, Issue 2, 2013, Pages 122-125

  Bootman, Martin D ^a,b   Rietdorf, Katja ^c   Collins, Tony ^d   Walker, Simon ^a   Sanderson, Michael ^c  

^a BABRAHAM INSTITUTE   (United Kingdom)

^b OPEN UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^d MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; CARBOXYLIC ACID; ESTERASE; FLUORESCENT DYE; FURA 2; INDICATOR; POLOXAMER; SULFINPYRAZONE; CALCIUM;

ARTICLE; CELL MEMBRANE; CELL TYPE; CULTURE MEDIUM; FLUORESCENCE IMAGING; GENETIC TRANSFECTION; IMAGIMG SYSTEM; LUNG SLICE; MICROINJECTION; PINOCYTOSIS; SIGNAL TRANSDUCTION; CHEMISTRY; DIFFUSION;

CALCIUM; CARBOXYLIC ACIDS; DIFFUSION; FLUORESCENT DYES; MICROINJECTIONS; PINOCYTOSIS;

MLCS; MLOWN;

EID: 84873373156     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot072801     Document Type: Article

References (4)

1. 2+ spikes in HeLa cells by sensitizing the inositol 1,4,5-trisphosphate receptor. J Biol Chem 267: 25113-25119.
2. 2+ imaging. Cold Spring Harb Protoc doi: 10.1101/pdb.top066050.
3. Perez JF, Sanderson MJ. 2005. The frequency of calcium oscillations induced by 5-HT, ACH, and KCl determine the contraction of smooth muscle cells of intrapulmonary bronchioles. J Gen Physiol 125: 535-553.
4. Tsien RY. 1981. A non-disruptive technique for loading calcium buffers and indicators into cells. Nature 290: 527-528.