Refolded recombinant Siglec5 for NMR investigation of complex carbohydrate binding

Protein Expression and Purification

Volumn 88, Issue 2, 2013, Pages 183-189

  Barb, Adam W ^a,b   Wang, Xu ^a,c   Prestegard, James H ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b IOWA STATE UNIVERSITY   (United States)

^c ARIZONA STATE UNIVERSITY   (United States)

Author keywords

Carbohydrate recognition; Glycoprotein; NMR chemical shift perturbation; On column refolding; Sialoside binding

Indexed keywords

DIFFERENTIATION ANTIGEN; DNA; LECTIN; LEUKOCYTE ANTIGEN; RECOMBINANT PROTEIN; SIALIC ACID DERIVATIVE; SIGLEC5 PROTEIN, HUMAN;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; PROTEIN BINDING; PROTEIN REFOLDING; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BINDING SITES; DNA; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; LECTINS; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN REFOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SIALIC ACIDS;

EID: 84873358322     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.01.005     Document Type: Article

References (21)

1. P.R. Crocker, J.C. Paulson, and A. Varki Siglecs and their roles in the immune system Nat. Rev. Immunol. 7 2007 255 266
2. M.K. O'Reilly, and J.C. Paulson Siglecs as targets for therapy in immune-cell-mediated disease Trends Pharmacol. Sci. 30 2009 240 248
3. S.J. McMillan, and P.R. Crocker CD33-related sialic-acid-binding immunoglobulin-like lectins in health and disease Carbohydrate Res. 343 2008 2050 2056
4. A. Varki Essentials Glycobiology 2009 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
5. C. Jones, M. Virji, and P.R. Crocker Recognition of sialylated meningococcal lipopolysaccharide by siglecs expressed on myeloid cells leads to enhanced bacterial uptake Mol. Microbiol. 49 2003 1213 1225
6. H. Rempel, C. Calosing, B. Sun, and L. Pulliam Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity PLoS One 3 2008 e1967
7. S. Han, B.E. Collins, P. Bengtson, and J.C. Paulson Homomultimeric complexes of CD22 in B cells revealed by protein-glycan cross-linking Nat. Chem. Biol. 1 2005 93 97
8. P. Gunnarsson, L. Levander, P. Pahlsson, and M. Grenegard The acute-phase protein alpha 1-acid glycoprotein (AGP) induces rises in cytosolic Ca2+ in neutrophil granulocytes via sialic acid binding immunoglobulin-like lectins (siglecs) FASEB J. 21 2007 4059 4069
9. A.F. Carlin, Y.C. Chang, T. Areschoug, G. Lindahl, N. Hurtado-Ziola, C.C. King, A. Varki, and V. Nizet Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5 J. Exp. Med. 206 2009 1691 1699
10. T. Nordstrom, E. Movert, A.I. Olin, S.R. Ali, V. Nizet, A. Varki, T. Areschoug, Human Siglec-5 inhibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein, J. Biol. Chem. 286 (2011) 33981-33991.
11. M.A. Zhuravleva, K. Trandem, and P.D. Sun Structural implications of Siglec-5-mediated sialoglycan recognition J. Mol. Biol. 375 2008 437 447
12. M. Jansson, Y.C. Li, L. Jendeberg, S. Anderson, B.T. Montelione, and B. Nilsson High-level production of uniformly 15N- and 13C-enriched fusion proteins in Escherichia coli J. Biomol. NMR 7 1996 131 141
13. C.T. Veldkamp, F.C. Peterson, P.L. Hayes, J.E. Mattmiller, J.C. Haugner 3rd, N. de la Cruz, and B.F. Volkman On-column refolding of recombinant chemokines for NMR studies and biological assays Protein Exp. Purif. 52 2007 202 209
14. A.P. Golovanov, G.M. Hautbergue, S.A. Wilson, and L.Y. Lian A simple method for improving protein solubility and long-term stability J. Am. Chem. Soc. 126 2004 8933 8939
15. F. Delaglio, S. Grzesiek, G.W. Vuister, G. Zhu, J. Pfeifer, and A. Bax NMRPipe: a multidimensional spectral processing system based on UNIX pipes J. Biomol. NMR 6 1995 277 293
16. B.A. Johnson Using NMR view to visualize and analyze the NMR spectra of macromolecules Meth. Mol. Biol. 278 2004 313 352
17. B. Han, Y.F. Liu, S.W. Ginzinger, and D.S. Wishart SHIFTX2: significantly improved protein chemical shift prediction J. Biomol. NMR 50 2011 43 57
18. Y. Shen, F. Delaglio, G. Cornilescu, and A. Bax TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts J. Biomol. NMR 44 2009 213 223
19. B.T. Farmer, K.L. Constantine, V. Goldfarb, M.S. Friedrichs, J. Yanchunas, J.G. Robertson, and L. Mueller Localizing the NADP+ binding site on the MurB enzyme by NMR Nat. Struct. Biol. 3 1996 995 997
20. J. Cavanagh Protein NMR Spectroscopy: Principles and Practice 2007 Academic Press Amsterdam, Boston
21. D. Sharma, and K. Rajarathnam C-13 NMR chemical shifts can predict disulfide bond formation J. Biomol. NMR 18 2000 165 171