uPAR regulates pericellular proteolysis through a mechanism involving integrins and fMLF-receptors

Thrombosis and Haemostasis

Volumn 109, Issue 2, 2013, Pages 309-318

  Montuori, Nunzia ^a   Cosimato, Vincenzo ^b   Rinaldi, Loredana ^b   Rea, Vincenza Elena Anna ^a   Alfano, Daniela ^b,c   Ragno, Pia ^b  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b UNIVERSITY OF SALERNO   (Italy)

^c INSTITUTE OF GENETICS AND BIOPHYSICS ADRIANO BUZZATI TRAVERSO   (Italy)

Author keywords

Pericellular proteolysis; Plasminogen activators; Urokinase receptor

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL) CHROMONE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL) IMIDAZOLE; COMPLEMENTARY DNA; FORMYLATED MLF PEPTIDE RECEPTOR; G PROTEIN COUPLED RECEPTOR; INTEGRIN; MITOGEN ACTIVATED PROTEIN KINASE; SMALL INTERFERING RNA; SYNAPTOPHYSIN; UNCLASSIFIED DRUG; UROKINASE; UROKINASE RECEPTOR;

ARTICLE; CONTROLLED STUDY; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WESTERN BLOTTING; ZYMOGRAPHY;

CHOLERA TOXIN; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; HEK293 CELLS; HELA CELLS; HUMANS; INTEGRINS; LIGANDS; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PERTUSSIS TOXIN; PROTEIN KINASE INHIBITORS; RECEPTORS, FORMYL PEPTIDE; RECEPTORS, UROKINASE PLASMINOGEN ACTIVATOR; RNA INTERFERENCE; SIGNAL TRANSDUCTION; TRANSFECTION; UP-REGULATION; UROKINASE-TYPE PLASMINOGEN ACTIVATOR;

EID: 84873278736     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1160/TH12-08-0546     Document Type: Article

References (36)

1. Ragno P. The urokinase receptor: a ligand or a receptor? Story of a sociable molecule. Cell Mol Life Sci 2006; 63: 1028-1037.
2. Ellis V, Scully MF, Kakkar VV. Plasminogen activation initiated by single-chain urokinase-type plasminogen activator. Potentiation by U937 monocytes. J Biol Chem 1989; 264: 2185-2188.
3. Montuori N, Ragno P. Multiple activities of a multifaceted receptor: roles of cleaved and soluble uPAR. Front Biosci 2009;14: 2494-2503.
4. Fazioli F, Resnati M, Sidenius N, et al. A urokinase-sensitive region of the human urokinase receptor is responsible for its chemotactic activity. EMBO J 1997;16: 7279-7286.
5. Resnati M, Pallavicini I, Wang JM, et al. The fibrinolytic receptor for urokinase activates the G protein-coupled chemotactic receptor FPRL1/LXA4R. Proc Natl Acad Sci USA 2002; 99: 1359-1364.
6. Huang J, Chen K, Gong W, et al. G-protein coupled chemoattractant receptors and cancer. Front Biosci 2008; 13: 3352-3363.
7. Montuori N, Carriero MV, Salzano S, et al. The cleavage of the urokinase receptor regulates its multiple functions. J Biol Chem 2002; 277: 46932-46939.
8. de Paulis A, Montuori N, Prevete N, et al. Urokinase induces basophil chemotaxis through a urokinase receptor epitope that is an endogenous ligand for formyl peptide receptor-like 1 and-like 2. J Immunol 2004; 173: 5739-5748.
9. D'Alessio S, Blasi F. The urokinase receptor as an entertainer of signal transduction. Front Biosci 2009; 14: 4575-4587.
10. Smith HW, Marshall CJ. Regulation of cell signalling by uPAR. Nat Rev Mol Cell Biol 2010; 11: 23-36.
11. Blasi F, Carmeliet P. uPAR: a versatile signalling orchestrator. Nat Rev Mol Cell Biol 2002; 3: 932-943.
12. Montuori N, Visconte V, Rossi G, et al. Soluble and cleaved forms of the urokinase-receptor: degradation products or active molecules? Thromb Haemost 2005; 93: 192-198.
13. Montuori N, Rossi G, Ragno P. Post-transcriptional regulation of gene expression in the plasminogen activation system. Biol Chem 2002; 383: 47-53.
14. Montuori N, Salzano S, Rossi G, et al. Urokinase-type plasminogen activator up-regulates the expression of its cellular receptor. FEBS Lett 2000; 476: 166-170.
15. Montuori N, Mattiello A, Mancini A, et al. Urokinase-type plasminogen activator up-regulates the expression of its cellular receptor through a post-transcriptional mechanism. FEBS Lett 2001; 508: 379-384.
16. Montuori N, Mattiello A, Mancini A, et al. Urokinase-mediated posttranscriptional regulation of urokinase-receptor expression in non small cell lung carcinoma. Int J Cancer 2003; 105: 353-360.
17. Rønne E, Behrendt N, Ellis V, et al. Cell-induced potentiation of the plasminogen activation system is abolished by a monoclonal antibody that recognizes the NH2-terminal domain of the urokinase receptor. FEBS Lett 1991; 288: 233-236.
18. Roldan AL, Cubellis MV, Masucci MT, et al. Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis. EMBO J 1990; 9: 467-474.
19. Vassalli JD, Dayer JM, Wohlwend A, et al. Concomitant secretion of prourokinase and of a plasminogen activator-specific inhibitor by cultured human monocytes-macrophages. J Exp Med 1984; 159: 1653-1668.
20. Ragno P, Estreicher A, Gos A, et al. Polarized secretion of urokinase-type plasminogen activator by epithelial cells. Exp Cell Res 1992; 203: 236-243.
21. Wei Y, Lukashev M, Simon DI, et al. Regulation of integrin function by the urokinase receptor. Science 1996; 273: 1551-1555.
22. Wei Y, Waltz DA, Rao N, et al. Identification of the urokinase receptor as an adhesion receptor for vitronectin. J Biol Chem 1994; 269: 32380-32388.
23. Madsen CD, Ferraris GM, Andolfo A, et al. uPAR-induced cell adhesion and migration: vitronectin provides the key. J Cell Biol 2007; 177: 927-939.
24. Besser D, Presta M, Nagamine Y. Elucidation of a signalling pathway induced by FGF-2 leading to uPA gene expression in NIH 3T3 fibroblasts. Cell Growth Differ 1995; 6: 1009-1017.
25. Silberman S, Janulis M, Schultz RM. Characterization of downstream Ras signals that induce alternative protease-dependent invasive phenotypes. J Biol Chem 1997; 272: 5927-5935.
26. Huang S, New L, Pan Z, et al. Urokinase plasminogen activator/urokinase-specific surface receptor expression and matrix invasion by breast cancer cells requires constitutive p38alpha mitogen-activated protein kinase activity. J Biol Chem 2000; 275: 12266-12272.
27. Montero L, Nagamine Y. Regulation by p38 mitogen-activated protein kinase of adenylate-and uridylate-rich element-mediated urokinase-type plasminogen activator (uPA) messenger RNA stability and uPA-dependent in vitro cell invasion. Cancer Res 1999; 59: 5286-5293.
28. Gyetko MR, Todd RF 3rd, Wilkinson CC, et al. The urokinase receptor is required for human monocyte chemotaxis in vitro. J Clin Invest 1994; 93: 1380-1387.
29. Schreiber RE, Prossnitz ER, Ye RD, et al. Reconstitution of recombinant N-formyl chemotactic peptide receptor with G protein. J Leukoc Biol 1993; 53: 470-474.
30. Wei Y, Eble JA, Wang Z, et al. Urokinase receptors promote beta1 integrin function through interactions with integrin alpha3beta1. Mol Biol Cell 2001; 12: 2975-2986.
31. Zannetti A, Del Vecchio S, Carriero MV, et al. Coordinate up-regulation of Sp1 DNA-binding activity and urokinase receptor expression in breast carcinoma. Cancer Res 2000; 60: 1546-1551.
32. Gargiulo L, Longanesi-Cattani I, Bifulco K, et al. Cross-talk between fMLP and vitronectin receptors triggered by urokinase receptor-derived SRSRY peptide. J Biol Chem 2005; 280: 25225-25232.
33. Chigaev A, Waller A, Amit O, et al. Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation. J Biol Chem 2009; 284: 14337-14346.
34. Ghosh S, Johnson JJ, Sen R, et al. Functional relevance of urinary-type plasminogen activator receptor-alpha3beta1 integrin association in proteinase regulatory pathways. J Biol Chem 2006; 281: 13021-13029.
35. Hildenbrand R, Schaaf A, Dorn-Beineke A, et al. Tumor stroma is the predominant uPA-, uPAR-, PAI-1-expressing tissue in human breast cancer: prognostic impact. Histol Histopathol 2009; 24: 869-877.
36. Grøndahl-Hansen J, Ralfkiaer E, Kirkeby LT, et al. Localization of urokinasetype plasminogen activator in stromal cells in adenocarcinomas of the colon in humans. Am J Pathol 1991; 138: 111-117.