Enlightening G-protein-coupled receptors on the plasma membrane using super-resolution photoactivated localization microscopy

Biochemical Society Transactions

Volumn 41, Issue 1, 2013, Pages 191-196

  Scarselli, Marco ^a   Annibale, Paolo ^b   Gerace, Claudio ^a   Radenovic, Aleksandra ^b  

^a UNIVERSITY OF PISA   (Italy)

^b EPFL   (Switzerland)

Author keywords

Clusters; G protein coupled receptors; Oligomerization; Photoactivated localization microscopy

Indexed keywords

ACTIN; G PROTEIN COUPLED RECEPTOR; GAG PROTEIN;

ACTIN FILAMENT; CELL MEMBRANE; CONFERENCE PAPER; FLUORESCENCE MICROSCOPY; OLIGOMERIZATION; PHOTOACTIVATED LOCALIZATION MICROSCOPY; PHOTOACTIVATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SPECTROMETRY; STOICHIOMETRY;

ANIMALS; CELL LINE; LIGHT; MEMBRANE PROTEINS; MICROSCOPY; RATS; RECEPTORS, G-PROTEIN-COUPLED;

EID: 84873123405     PISSN: 03005127     EISSN: 14708752     Source Type: Journal    
DOI: 10.1042/BST20120250     Document Type: Conference Paper

References (26)

1. Hell, S. W. (2009) Microscopy and its focal switch. Nat. Methods 6, 24-32
2. Harding, A. S. and Hancock, J. F. (2008) Using plasma membrane nanoclusters to build better signaling circuits. Trends Cell Biol. 18, 364-371
3. Manz, B. N. and Groves, J. T. (2010) Spatial organization and signal transduction at intercellular junctions. Nat. Rev. Mol. Cell Biol. 11, 342-352
4. Bethani, I., Skånland, S. S., Dikic, I. and Acker-Palmer, A. (2010) Spatial organization of transmembrane receptor signaling. EMBO J. 29, 2677-2688
5. Patel, H. H., Murray, F. and Insel, P. A. (2008) G-protein-coupled receptorsignaling components in membrane raft and caveolae microdomains. Handb. Exp. Pharmacol. 186, 167-184
6. Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J. and Hess, H. F. (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642-1645 (Pubitemid 44414039)
7. Rust, M. J., Bates, M. and Zhuang, X. (2006) Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (storm). Nat. Methods 3, 793-796
8. Hell, S. W. and Wichmann, J. (1994) Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescencemicroscopy. Opt. Lett. 11, 780-782
9. Hess, S. T., Girirajan, T. P. and Mason, M. D. (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 11, 4258-4272 (Pubitemid 44887284)
10. Fotiadis, D., Scheuring, S., Muller, S. A., Engel, A. and Muller, D. J. (2002) Imaging and manipulation of biological structures with the AFM. Micron 33, 385-397 (Pubitemid 34231290)
11. Ianoul, A., Grant, D. D., Rouleau, Y., Bani-Yaghoub, M., Johnston, L. J. and Pezacki, J. P. (2005) Imaging nanometer domains of β-adrenergic receptor complexes on the surface of cardiac myocytes. Nat. Chem. Biol. 1, 196-202
12. Powell, R. D., Halsey, C. M. and Hainfeld, J. F. (1998) Combined fluorescent and gold immunoprobes. Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech. 42, 2-12 (Pubitemid 28363790)
13. Tanaka, K. A., Suzuki, K. G., Shirai, Y. M., Shibutani, S. T., Miyahara, M. S., Tsuboi, H., Yahara, M., Yoshimura, A., Mayor, S., Fujiwara, T. K. and Kusumi, A. (2010) Membrane molecules mobile even after chemical fixation. Nat. Methods 11, 865-866
14. Greenfield, D., McEvoy, A. L., Shroff, H., Crooks, G. E., Wingreen, N. S., Betzig, E. and Liphardt, J. (2009) Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy. PLoS Biol. 7, e1000137
15. Annibale, P., Vanni, S., Scarselli, M., Rothlisberger, U. and Radenovic, A. (2011) Quantitative photo activated localization microscopy: unraveling the effects of photoblinking. PLoS ONE 6, e22678
16. Annibale, P., Vanni, S., Scarselli, M., Rothlisberger, U. and Radenovic, A. (2011) Identification of clustering artifacts in photoactivated localization microscopy. Nat. Methods 8, 527-528
17. Sengupta, P. and Lippincott-Schwartz, J. (2012) Quantitative analysis of photoactivated localization microscopy (PALM) datasets using pair-correlation analysis. Bioessays 5, 396-405
18. 2-adrenergic receptor clusters identified using photoactivated localization microscopy are not lipid raft related, but depend on actin cytoskeleton integrity. J. Biol. Chem. 20, 16768-16780
19. Ganguly, S., Clayton, A. H. and Chattopadhyay, A. (2011) Organization of higher-order oligomers of the serotonin1 (A) receptorexplored utilizing homo-FRET in live cells. Biophys. J. 100, 361-368
20. 2-adrenergic receptors in the plasma membrane of cardiomyocyte-like H9c2 cells is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins but not caveolae. Mol. Biol. Cell 22, 2970-2982
21. Wheeler, D., Sneddon, W. B., Wang, B., Friedman, P. A. and Romero, G. (2007) NHERF-1 and the cytoskeleton regulate the traffic and membrane dynamics of G protein-coupled receptors. J. Biol. Chem. 34, 25076-25087 (Pubitemid 47347543)
22. Sengupta, P., Jovanovic-Talisman, T., Skoko, D., Renz, M., Veatch, S. L. and Lippincott-Schwartz, J. (2011) Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis. Nat. Methods 11, 969-975
23. Shroff, H., Galbraith, C. G., Galbraith, J. A. and Betzig, E. (2008) Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat. Methods 5, 417-423 (Pubitemid 351619100)
24. Patterson, G., Davidson, M., Manley, S. and Lippincott-Schwartz, J. (2012) Superresolution imaging using single-molecule localization. Annu. Rev. Phys. Chem. 61, 345-367
25. Subach, F. V., Patterson, G. H., Manley, S., Gillette, J. M., Lippincott-Schwartz, J. and Verkhusha, V. V. (2009) Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nat. Methods 2, 153-159
26. Yao, J., Fetter, R. D., Hu, P., Betzig, E. and Tjian, R. (2011) Subnuclear segregation of genes and core promoter factors in myogenesis. Genes Dev. 6, 569-580