Real-time cell viability assays using a new anthracycline derivative DRAQ7®

Cytometry Part A

Volumn 83 A, Issue 2, 2013, Pages 227-234

  Akagi, Jin ^a   Kordon, Magdalena ^b   Zhao, Hong ^c   Matuszek, Anna ^a   Dobrucki, Jurek ^b   Errington, Rachel ^d   Smith, Paul J ^d   Takeda, Kazuo ^e   Darzynkiewicz, Zbigniew ^c   Wlodkowic, Donald ^a,f  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b JAGIELLONIAN UNIVERSITY   (Poland)

^c NEW YORK MEDICAL COLLEGE   (United States)

^d CARDIFF UNIVERSITY   (United Kingdom)

^e On Chip Biotechnologies Co Ltd   (Japan)

^f RMIT UNIVERSITY   (Australia)

Author keywords

Cell cycle; Cell viability; Cytometry; Cytotoxicity; DNA damage response; DRAQ7; Drug; Microfluidic; Real time assays

Indexed keywords

4 [4 (4' CHLORO 2 BIPHENYLYLMETHYL) 1 PIPERAZINYL] N [4 [3 DIMETHYLAMINO 1 (PHENYLTHIOMETHYL)PROPYLAMINO] 3 NITROBENZENESULFONYL]BENZAMIDE; ABT-737; ANTHRACYCLINE; BENZAMIDE DERIVATIVE; BIPHENYL DERIVATIVE; DACTINOMYCIN; DRAQ7 FLUORESCENT PROBE; ETOPOSIDE; FLUORESCENT DYE; H2AFX PROTEIN, HUMAN; HISTONE; NITROPHENOL; PIPERAZINE DERIVATIVE; RHODAMINE; STAUROSPORINE; SULFONAMIDE; SULFONE; TETRAMETHYLRHODAMINE METHYL ESTER; TW 37 COMPOUND; TW-37 COMPOUND;

APOPTOSIS; ARTICLE; CELL SURVIVAL; CHEMISTRY; DNA DAMAGE; DRUG EFFECT; FLOW CYTOMETRY; HUMAN; IC 50; METABOLISM; MITOCHONDRIAL MEMBRANE POTENTIAL; TUMOR CELL LINE;

ANTHRACYCLINES; APOPTOSIS; BENZAMIDES; BIPHENYL COMPOUNDS; CELL LINE, TUMOR; CELL SURVIVAL; DACTINOMYCIN; DNA DAMAGE; ETOPOSIDE; FLOW CYTOMETRY; FLUORESCENT DYES; HISTONES; HUMANS; INHIBITORY CONCENTRATION 50; MEMBRANE POTENTIAL, MITOCHONDRIAL; NITROPHENOLS; PIPERAZINES; RHODAMINES; STAUROSPORINE; SULFONAMIDES; SULFONES;

EID: 84873093379     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22228     Document Type: Article

References (26)

1. Wlodkowic D, Skommer J, Pelkonen J. Multiparametric analysis of HA14-1-induced apoptosis in follicular lymphoma cells. Leuk Res 2006; 30: 1187-1192.
2. Wlodkowic D, Skommer J, Pelkonen J. Brefeldin A triggers apoptosis associated with mitochondrial breach and enhances HA14-1- and anti-Fas-mediated cell killing in follicular lymphoma cells. Leuk Res 2007; 31: 1687-700.
3. Wlodkowic D, Skommer J, Darzynkiewicz Z. Cytometry in cell necrobiology revisited. Recent advances and new vistas. Cytometry Part A 2010; 77A: 591-606.
4. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F. Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 1997; 27: 1-20.
5. Khoshmanesh K, Akagi J, Nahavandi S, Skommer J, Baratchi S, Cooper JM, Kalantar-Zadeh K, Williams DE, Wlodkowic D. Dynamic analysis of drug-induced cytotoxicity using chip-based dielectrophoretic cell immobilization technology. Anal Chem 2011; 83: 2133-2144.
6. Zhao H, Oczos J, Janowski P, Trembecka D, Dobrucki J, Darzynkiewicz Z, Wlodkowic D. Rationale for the real-time and dynamic cell death assays using propidium iodide. Cytometry Part A 2010; 77A: 399-405.
7. Skommer J, Darzynkiewicz Z, Wlodkowic D. Cell death goes LIVE: Technological advances in real-time tracking of cell death. Cell Cycle 2010; 9: 2330-2341.
8. Skommer J, Raychaudhuri S, Wlodkowic D. Timing is everything: Stochastic origins of cell-to-cell variability in cancer cell death. Front Biosci 2011; 16: 307-314.
9. Skommer J, Brittain T, Raychaudhuri S. Bcl-2 inhibits apoptosis by increasing the time-to-death and intrinsic cell-to-cell variations in the mitochondrial pathway of cell death. Apoptosis 2010; 15: 1223-1233.
10. Hawkins ED, Markham JF, McGuinness LP, Hodgkin PD. A single-cell pedigree analysis of alternative stochastic lymphocyte fates. Proc Natl Acad Sci USA 2009; 106: 13457-13462.
11. Raychaudhuri S, Willgohs E, Nguyen TN, Khan EM, Goldkorn T. Monte Carlo simulation of cell death signaling predicts large cell-to-cell stochastic fluctuations through the type 2 pathway of apoptosis. Biophys J 2008; 95: 3559-3562.
12. Wlodkowic D, Faley S, Darzynkiewicz Z, Cooper JM. Real-time cytotoxicity assays. Meth Mol Biol 2011; 731: 285-291.
13. Khoshmanesh K, Akagi J, Nahavandi S, Kalantar-zadeh K, Baratchi S, Williams DE, Cooper JM, Wlodkowic D. Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis. Anal Chem 2011; 83: 3217-3221.
14. Wlodkowic D, Faley S, Skommer J, McGuinness D, Cooper JM. Biological implications of polymeric microdevices for live cell assays. Anal Chem 2009; 81: 9828-9833.
15. Kanda T, Sullivan KF, Wahl GM. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr Biol 1998; 8: 377-385.
16. Zhao H, Rybak P, Dobrucki J, Traganos F, Darzynkiewicz Z. Relationship of DNA damage signaling to DNA replication following treatment with DNA topoisomerase inhibitors camptothecin/topotecan, mitoxantrone, or etoposide. Cytometry. Part A 2012; 81A: 45-51.
17. Zhao H, Dobrucki J, Rybak P, Traganos F, Dorota Halicka H, Darzynkiewicz Z. Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using "click chemistry". Cytometry Part A 2011; 79A: 897-902.
18. Takao M, Takeda K. Enumeration, characterization, and collection of intact circulating tumor cells by cross contamination-free flow cytometry. Cytometry A 2011; 79A: 107-117.
19. Wlodkowic D, Khoshmanesh K, Sharpe JC, Darzynkiewicz Z, Cooper JM. Apoptosis goes on a chip: Advances in the microfluidic analysis of programmed cell death. Anal Chem 2011; 83: 6439-6446.
20. Wlodkowic D, Cooper JM. Tumors on chips: Oncology meets microfluidics. Curr Opin Chem Biol 2010; 14: 556-567.
21. Zhao H, Traganos F, Dobrucki J, Wlodkowic D, Darzynkiewicz Z. Induction of DNA damage response by the supravital probes of nucleic acids. Cytometry Part A 2009; 75A: 510-519.
22. Wlodkowic D, Skommer J, McGuinness D, Faley S, Kolch W, Darzynkiewicz Z, Cooper JM. Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells. Anal Chem 2009; 81: 6952-6959.
23. Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper JM. Dynamic analysis of apoptosis using cyanine SYTO probes: From classical to microfluidic cytometry. Exp Cell Res 2009; 315: 1706-1714.
24. Floryk D, Houstek J. Tetramethyl rhodamine methyl ester (TMRM) is suitable for cytofluorometric measurements of mitochondrial membrane potential in cells treated with digitonin. Biosci Reports 1999; 19: 27-34.
25. Skommer J, Wlodkowic D, Matto M, Eray M, Pelkonen J. HA14-1, a small molecule Bcl-2 antagonist, induces apoptosis and modulates action of selected anticancer drugs in follicular lymphoma B cells. Leuk Res 2006; 30: 322-331.
26. Zhao H, Halicka HD, Traganos F, Jorgensen E, Darzynkiewicz Z. New biomarkers probing depth of cell senescence assessed by laser scanning cytometry. Cytometry Part A 2010; 77A: 999-1007.