OMIP-016: Characterization of antigen-responsive macaque and human T-cells

Cytometry Part A

Volumn 83 A, Issue 2, 2013, Pages 182-184

  Guenounou, Sabrina ^a,b,c   Bosquet, Nathalie ^a,b,c   Dembek, Claudia J ^d   Le Grand, Roger ^a,b,c   Cosma, Antonio ^a,b,c  

^a INSTITUT DE RADIOPROTECTION ET DE SÛRETÉ NUCLÉAIRE   (France)

^b UNIVERSITÉ PARIS SACLAY   (France)

^c Vaccine Research Institute   (France)

^d INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

HIV; Intracellular cytokines; SIV; T cells; Vaccines

Indexed keywords

CD40 LIGAND; CYTOKINE; DIFFERENTIATION ANTIGEN; MACROPHAGE INFLAMMATORY PROTEIN 1BETA;

ANIMAL; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL SEPARATION; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; MACACA FASCICULARIS; METABOLISM; METHODOLOGY;

ANIMALS; ANTIGENS, DIFFERENTIATION; CD4-POSITIVE T-LYMPHOCYTES; CD40 LIGAND; CD8-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; CHEMOKINE CCL4; CYTOKINES; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MACACA FASCICULARIS;

EID: 84873084893     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22233     Document Type: Article

References (4)

1. Shedlock DJ, Silvestri G, Weiner DB. Monkeying around with HIV vaccines: Using rhesus macaques to define 'gatekeepers' for clinical trials. Nat Rev Immunol 2009; 9: 717-728.
2. Kutscher S, Dembek CJ, Allgayer S, Heltai S, Stadlbauer B, Biswas P, Nozza S, Tambussi G, Bogner JR, Stellbrink HJ, et al. The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting providing a sensitive alternative to the ELISPOT. AIDS Res Ther 2008; 5: 22.
3. Betts MR, Nason MC, West SM, De Rosa SC, Migueles SA, Abraham J, Lederman MM, Benito JM, Goepfert PA, Connors M, et al. HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells. Blood 2006; 107: 4781-4789.
4. Frentsch M, Arbach O, Kirchhoff D, Moewes B, Worm M, Rothe M, Scheffold A, Thiel A. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat Med 2005; 11: 1118-1124.