Studies of loose protein aggregates by flow field-flow fractionation (FFF) coupled to multi-angle laser light scattering (MALLS)

Field-Flow Fractionation in Biopolymer Analysis

Volumn , Issue , 2012, Pages 103-112

  Palais, Caroline ^a   Capelle, Martinus ^a   Arvinte, Tudor ^a  

^a Therapeomic Inc   (Switzerland)

Author keywords

Antibody aggregation; Effects of cross flow; Effects of focus flow; Flow field flow fractionation; Freeze thaw cycles; Loose aggregates; Protein aggregation; Protein polysaccharide conjugate; Stability studies

Indexed keywords

ANTIBODIES; FLOW FIELDS; FRACTIONATION; LIGHT SCATTERING; LIQUID CHROMATOGRAPHY;

ANTIBODY AGGREGATION; CROSS FLOWS; EFFECTS OF FOCUS FLOW; FLOW FIELD FLOW FRACTIONATIONS; FREEZE-THAW CYCLES; PROTEIN AGGREGATION; STABILITY STUDY;

AGGREGATES;

EID: 84872858353     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-3-7091-0154-4_7     Document Type: Chapter

References (3)

1. Demeule B, Palais C, Machaidze G, Gumy R, Arvinte R (2009) New methods allowing the detection of protein aggregates: a case study on trastuzumab. MAbs 1(2):142-150
2. Arakawa T, Philo JS, Ejima D, Sato H, Tsumoto K (2007) Aggregation analysis of therapeutic proteins, part 3; principles and optimization of field-flow fractionation (FFF). BioProcess Int 5(10):52-70
3. Demeule B, Gurny R, Arvinte T (2007) Detection and characterization of protein aggregates by fluorescence microscopy. Int J Pharm 329(1-2):37-45