Construction of an unmarked Zymomonas mobilis mutant using a site-specific FLP recombinase

Food Technology and Biotechnology

Volumn 50, Issue 4, 2012, Pages 406-411

  Zou, Shao Lan ^a   Hong, Jie Fang ^a   Wang, Cui ^a   Jing, Xin ^a   Zhang, Min Hua ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

Flippase expression; Homologous recombination; Transformation; Unmarked mutants; Zymomonas mobilis

Indexed keywords

FLIPPASE EXPRESSION; HOMOLOGOUS RECOMBINATION; TRANSFORMATION; UNMARKED MUTANTS; ZYMOMONAS MOBILIS;

GENE EXPRESSION; GENETIC ENGINEERING; YEAST;

GENE ENCODING;

SACCHAROMYCES CEREVISIAE; ZYMOMONAS MOBILIS;

EID: 84872744524     PISSN: 13309862     EISSN: 13342606     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. H. Yun, B. G. Kim, Enzymatic production of (R)-phenylacetylcarbinol by pyruvate decarboxylase from Zymomonas mobilis, Biotechnol. Bioprocess. Eng. 13 (2008) 372-376. (Pubitemid 352037722)
2. M. A. Hermans, B. Neuss, H. Sahm, Content and composition of hopanoids in Zymomonas mobilis under various growth conditions, J. Bacteriol. 173 (1991) 5592-5595.
3. J. Swings, J. De Ley, The biology of Zymomonas, Bacteriol. Rev. 41 (1977) 1-46. (Pubitemid 8082285)
4. P. L. Rogers, Y. J. Jeon, K. J. Lee, H. G. Lawford, Zymomonas mobilis for fuel ethanol and higher value products, Adv. Biochem. Eng. Biotechnol. 108 (2007) 263-288. (Pubitemid 47439712)
5. B. S. Montenecourt: Zymomonas, A Unique Genus of Bacteria. In: Biology of Industrial Microorganisms, A. L. Demain, N. A. Solomon (Eds.), Benjamin/Cummings, Menlo Park, CA, USA (1985) pp. 261-289.
6. G. A. Sprenger, M. A. Typas, C. Drainas, Genetics and genetic engineering of Zymomonas mobilis, World J. Microbiol. Biotechnol. 9 (1993) 17-24.
7. T. Okamoto, K. Nakamura, Simple and highly efficient transformation method for Zymomonas mobilis: Electroporation, Biosci. Biotechnol. Biochem. 56 (1992) 833.
8. C. K. Lam, P. O'Mullan, D. E. Eveleigh, Transformation of Zymononas mobilis by electroporation, Appl. Microbiol. Biotechnol. 39 (1993) 305-308. (Pubitemid 23167179)
9. C. C. Liang, W. C. Lee, Characteristics and transformation of Zymomonas mobilis with plasmid pKT230 by electroporation, Bioprocess Biosyst. Eng. 19 (1998) 81-85.
10. Y. J. Jeon, C. J. Svenson, E. L. Joachimsthal, P. L. Rogers, Kinetic analysis of ethanol production by an acetate-resistant strain of recombinant Zymomonas mobilis, Biotechnol. Lett. 24 (2002) 819-824. (Pubitemid 34600959)
11. Y. J. Jeon, C. J. Svenson, P. L. Rogers, Over-expression of xylulokinase in a xylose-metabolising recombinant strain of Zymomonas mobilis, FEMS Microbiol. Lett. 244 (2005) 85-92. (Pubitemid 40267702)
12. U. Kalnenieks, N. Galinina, M. M. Torna, J. L. Pickford, R. Rutkis, R. K. Poole, Respiratory behaviour of a Zymomonas mobilis adhB::kanr mutant supports the hypothesis of two alcohol dehydrogenase isoenzymes catalysing opposite reactions, FEBS Lett. 580 (2006) 5084-5088. (Pubitemid 44332383)
13. S. H. Yang, D. A. Pelletier, T. Y. S. Lu, S. D. Brown, The Zymomonas mobilis regulator hfcj contributes to tolerance against multiple lignocellulosic pretreatment inhibitors, BMC Microbiol. 10 (2010) 135.
14. H. P. Schweizer, Applications of the Saccharomyces cerevisiae Flp-FRT system in bacterial genetics, J. Mol. Microbiol. Biotechnol. 5 (2003) 67-77. (Pubitemid 37475749)
15. K. A. Datsenko, B. L. Wanner, One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products, PNAS, 97 (2000) 6640-6645. (Pubitemid 30412767)
16. N. G. Copeland, N. A. Jenkins, D. L. Court, Recombineering: A powerful new tool for mouse functional genomics, Nat. Rev. Genet. 2 (2001) 769-779. (Pubitemid 33685574)
17. S. Yang, M. L. Land, D. M. Klingeman, D. A. Pelletier, T. Y. S. Lu, S. L. Martin et al., Paradigm for industrial strain improvement identifies sodium acetate tolerance loci in Zymomonas mobilis and Saccharomyces cerevisiae, PNAS, 107 (2010) 10395-10400.
18. V. De Lorenzo, M. Herrero, U. Jakubzik, K. N. Timmis, Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria, J. Bacteriol. 172 (1990) 6568-6572. (Pubitemid 20372802)
19. J. S. Seo, H. Chong, H. S. Park, K. O. Yoon, C. Jung, J. J. Kim et al., The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4, Nat. Biotechnol. 23 (2005) 63-68. (Pubitemid 41724626)
20. S. L. Zou, J. F. Hong, Y. Y. Ma, Y. T. Zhang, K. Zhang, M. H. Zhang, Construction of a specific integrating plasmid for Zymomonas mobilis, Nankai Daxue Xuebao, 42 (2009) 42-47 (in Chinese).
21. M. E. Kovach, R. H. Elzer, D. S. Hill, G. T. Robertson, M. A. Farris, R. M. Roop II, K. M. Peterson, Four new derivatives of the broad-host-range cloning vector pBBRlMCS, carrying different antibiotic-resistance cassettes, Gene, 166 (1995) 175-176.
22. J. Sambrook, D. W. Russel: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA (2001).
23. L. P. Yomano, R. K. Scopes, L. O. Ingram, Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli, J. Bacteriol. 175 (1993) 3926-3933. (Pubitemid 23206884)
24. M. Zhang, Y. C. Chou, X. K. Lai, S. Milstrey, N. Danielson, K. Evans et al., Recent advances in the development of Zymomonas mobilis for pentose utilization, Abstracts of the 21st Symposium on Biotechnology for Fuels and Chemistry, Fort Collins, CO, USA (1999) 2-16.
25. H. G. Lawford, J. D. Rousseau, Steady-state measurements of lactic acid production in a wild-type and a putative D-lactic acid dehydrogenase-negative mutant of Zymomonas mobilis: Influence of glycolytic flux, Appl. Biochem. Biotechnol. 98 (2002) 215-228. (Pubitemid 34460640)
26. * for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilis, J. Bacteriol. 174 (1992) 7370-7378.
27. K. B. Song, J. W. Seo, S. K. Rhee, Transcriptional analysis of levll operon encoding saccharolytic enzymes and two apparent genes involved in amino acid biosynthesis in Zymomonas mobilis, Gene, 232 (1999) 107-114.
28. A. Christogianni, E. Douka, A. I. Koukkou, E. Hatziloukas, C. Drainas, Transcriptional analysis of a gene cluster involved in glucose tolerance in Zymomonas mobilis: Evidence for an osmoregulated promoter, J. Bacteriol. 187 (2005) 5179-5188. (Pubitemid 41022936)
29. S. L. Zou, M. H. Zhang, J. F. Hong, Y. Y. Ma, W. N. Zhang, Comparison of the electrotransformation of plasmids and plasmid stability between Zymomonas mobilis ZM4 and CP4, Afr. J. Microbiol. Res. 5 (2011) 2026-2033.
30. W. O. Barneil, J. Liu, T. L. Hesman, M. C. O'Neill, T. Conway, The Zymomonas mobilis glf, zwf, edd, and glk genes form an operon: Localization of the promoter and identification of a conserved sequence in the regulatory region, J. Bacteriol. 174 (1992) 2816-2823.
31. K. Deanda, M. Zhang, C. Eddy, S. Picataggio, Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering, Appl. Environ. Microbiol. 62 (1996) 4465-4470. (Pubitemid 26399442)
32. N. Bernard, T. Ferain, D. Garmyn, P. Hols, J. Delcour, Cloning of the D-lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli, FEBS Lett. 290 (1991) 61-64.