Entamoeba histolytica: The over expression of a mutated EhRabB protein produces a decrease of in vitro and in vivo virulence

Experimental Parasitology

Volumn 133, Issue 3, 2013, Pages 339-345

  Juarez Hernandez L J ^a,b   Garcia Perez R M ^a   Salas Casas, A ^a,c   Garcia Rivera G ^a   Orozco, E ^a   Rodriguez M A ^a  

^a DEPARTAMENTO DE FÍSICA   (Mexico)

^b SISSA   (Italy)

^c UNIVERSIDAD AUTÓNOMA DEL ESTADO DE HIDALGO   (Mexico)

Author keywords

Dominant negative mutant; EhRabB; Entamoeba histolytica; Phagocytosis; Rab GTPase; Virulence

Indexed keywords

EHRABB PROTEIN; RAB PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; CYTOPATHOGENIC EFFECT; EHRABB GENE; ENTAMOEBA HISTOLYTICA; GENE; HAMSTER; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PARASITE VIRULENCE; PHAGOCYTOSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; TROPHOZOITE;

ANIMALS; BLOTTING, WESTERN; CRICETINAE; DOGS; ENTAMOEBA HISTOLYTICA; ERYTHROCYTES; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; GTP PHOSPHOHYDROLASES; HUMANS; LIVER ABSCESS, AMEBIC; MUTAGENESIS; PHAGOCYTOSIS; PROTOZOAN PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION; VIRULENCE;

CRICETINAE; ENTAMOEBA HISTOLYTICA; PROTOZOA;

EID: 84872691075     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2012.12.008     Document Type: Article

References (39)

1. Arroyo R., Orozco E. Localization and identification of an Entamoeba histolytica adhesin. Molecular and Biochemical Parasitology 1987, 23:151-158.
2. Bracha R., Mirelman D. Virulence of Entamoeba histolytica trophozoites. Effects of bacteria, microaerobic conditions, and metronidazole. Journal of Experimental Medicine 1984, 160:353-368.
3. Bruchhaus I., Roeder T., Lotter H., Schwerdtfeger M., Tannich E. Differential gene expression in Entamoeba histolytica isolated from amoebic liver abscess. Molecular Microbiology 2002, 44:1063-1072.
4. Chen X., Edwards J.A., Logsdon C.D., Ernst S.A., Williams J.A. Dominant negative Rab3D inhibits amylase release from mouse pancreatic acini. Journal of Biological Chemistry 2002, 277:18002-18009.
5. Debnath A., Tashker J.S., Sajid M., McKerrow J.H. Transcriptional and secretory responses of Entamoeba histolytica to mucins, epithelial cells and bacteria. International Journal for Parasitology 2007, 37:897-906.
6. Diamond L.S., Harlow D.R., Cunnick C.C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Transactions of the Royal Society of Tropical Medicine and Hygiene 1978, 72:431-432.
7. Feng Y., Press B., Wandinger-Ness A. Rab 7: an important regulator of late endocytic membrane traffic. Journal of Cell Biology 1995, 131:1435-1452.
8. Guzman-Medrano R., Castillo-Juarez B.A., Garcia-Perez R.M., Salas-Casas A., Orozco E., Rodriguez M.A. Entamoeba histolytica: alterations in EhRabB protein in a phagocytosis deficient mutant correlate with the Entamoeba dispar RabB sequence. Experimental Parasitology 2005, 110:259-264.
9. Hamann L., Nickel R., Tannich E. Transfection and continuous expression of heterologous genes in the protozoan parasite Entamoeba histolytica. Proceedings of the National Academy of Sciences USA 1995, 92:8975-8979.
10. Haque R., Huston C.D., Hughes M., Houpt E., Petri W.A. Amebiasis. New England Journal of Medicine 2003, 348:1565-1573.
11. Hermann-Le Denmat S., Jacquet M. Yeast RAS2 mutations modulating the ras-guanine exchange factor interaction. FEBS Letters 1997, 403:95-99.
12. Hume A.N., Collinson L.M., Rapak A., Gomes A.Q., Hopkins C.R., Seabra M.C. Rab27a regulates the peripheral distribution of melanosomes in melanocytes. Journal of Cell Biology 2001, 152:795-808.
13. Kyei G.B., Vergne I., Chua J., Roberts E., Harris J., Junutula J.R., Deretic V. Rab14 is critical for maintenance of Mycobacterium tuberculosis phagosome maturation arrest. EMBO Journal 2006, 25:5250-5259.
14. Lazzarino D.A., Blier P., Mellman I. The monomeric guanosine triphosphatase rab4 controls an essential step on the pathway of receptor-mediated antigen processing in B cells. Journal of Experimental Medicine 1998, 188:1769-1774.
15. Lynch E.C., Rosenberg I.M., Gitler C. An ion-channel forming protein produced by Entamoeba histolytica. EMBO Journal 1982, 1:801-804.
16. Marion S., Laurent C., Guillen N. Signalization and cytoskeleton activity through myosin IB during the early steps of phagocytosis in Entamoeba histolytica: a proteomic approach. Cell Microbiology 2005, 7:1504-1518.
17. Martinez-Lopez C., Orozco E., Sanchez T., Garcia-Perez R.M., Hernandez-Hernandez F., Rodriguez M.A. The EhADH112 recombinant polypeptide inhibits cell destruction and liver abscess formation by Entamoeba histolytica trophozoites. Cell Microbiology 2004, 6:367-376.
18. Mitra B.N., Saito-Nakano Y., Nakada-Tsukui K., Sato D., Nozaki T. Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica. Cell Microbiology 2007, 9:2112-2125.
19. Nozaki T., Nakada-Tsukui K. Membrane trafficking as a virulence mechanism of the enteric protozoan parasite Entamoeba histolytica. Parasitology Research 2006, 98:179-183.
20. Okada M., Huston C.D., Mann B.J., Petri W.A., Kita K., Nozaki T. Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica. Eukaryotic Cell 2005, 4:827-831.
21. Okada M., Huston C.D., Oue M., Mann B.J., Petri W.A., Kita K., Nozaki T. Kinetics and strain variation of phagosome proteins of Entamoeba histolytica by proteomic analysis. Molecular and Biochemical Parasitology 2006, 145:171-183.
22. Orozco E., Guarneros G., Martinez-Palomo A., Sanchez T. Entamoeba histolytica. Phagocytosis as a virulence factor. Journal of Experimental Medicine 1983, 158:1511-1521.
23. Petri W.A., Smith R.D., Schlesinger P.H., Murphy C.F., Ravdin J.I. Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. Journal of Clinical Investigation 1987, 80:1238-1244.
24. Picazarri K., Luna-Arias J.P., Carrillo E., Orozco E., Rodriguez M.A. Entamoeba histolytica: identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB. Experimental Parasitology 2005, 110:253-258.
25. Que X., Reed S.L. Cysteine proteinases and the pathogenesis of amebiasis. Clinical Microbiology Reviews 2000, 13:196-206.
26. Ravdin J.I., Croft B.Y., Guerrant R.L. Cytopathogenic mechanisms of Entamoeba histolytica. Journal of Experimental Medicine 1980, 152:377-390.
27. Riley K.N., Maldonado A.E., Tellier P., D'Souza-Schorey C., Herman I.M. Betacap73-ARF6 interactions modulate cell shape and motility after injury in vitro. Molecular Biology of the Cell 2003, 14:4155-4161.
28. Rodriguez M.A., Orozco E. Isolation and characterization of phagocytosis- and virulence-deficient mutants of Entamoeba histolytica. Journal of Infectious Diseases 1986, 154:27-32.
29. Rodriguez M.A., Garcia-Perez R.M., Garcia-Rivera G., Lopez-Reyes I., Mendoza L., Ortiz-Navarrete V., Orozco E. An Entamoeba histolytica rab-like encoding gene and protein: function and cellular location. Molecular and Biochemical Parasitology 2000, 108:199-206.
30. Romero-Diaz M., Gomez C., Lopez-Reyes I., Martinez M.B., Orozco E., Rodriguez M.A. Structural and functional analysis of the Entamoeba histolytica EhrabB gene promoter. BMC Molecular Biology 2007, 8:82.
31. Saito-Nakano Y., Nakazawa M., Shigeta Y., Takeuchi T., Nozaki T. Identification and characterization of genes encoding novel Rab proteins from Entamoeba histolytica. Molecular and Biochemical Parasitology 2001, 116:219-222.
32. Saito-Nakano Y., Loftus B.J., Hall N., Nozaki T. The diversity of Rab GTPases in Entamoeba histolytica. Experimental Parasitology 2005, 110:244-252.
33. Sambrook J., Russell D. Molecular Cloning. A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York.
34. Temesvari L.A., Harris E.N., Stanley S.L., Cardelli J.A. Early and late endosomal compartments of Entamoeba histolytica are enriched in cysteine proteases, acid phosphatase and several Ras-related Rab GTPases. Molecular and Biochemical Parasitology 1999, 103:225-241.
35. Tsutsumi V., Mena-Lopez R., Anaya-Velazquez F., Martinez-Palomo A. Cellular bases of experimental amebic liver abscess formation. American Journal of Pathology 1984, 117:81-91.
36. Welter B.H., Temesvari L.A. Overexpression of a mutant form of EhRabA, a unique Rab GTPase of Entamoeba histolytica, alters endoplasmic reticulum morphology and localization of the Gal/GalNAc adherence lectin. Eukaryotic Cell 2009, 8:1014-1026.
37. Welter B.H., Laughlin R.C., Temesvari L.A. Characterization of a Rab7-like GTPase, EhRab7: a marker for the early stages of endocytosis in Entamoeba histolytica. Molecular and Biochemical Parasitology 2002, 121:254-264.
38. Welter B.H., Powell R.R., Leo M., Smith C.M., Temesvari L.A. A unique Rab GTPase, EhRabA, is involved in motility and polarization of Entamoeba histolytica cells. Molecular and Biochemical Parasitology 2005, 140:161-173.
39. Zerial M., McBride H. Rab proteins as membrane organizers. Nature Reviews Molecular Cell Biology 2001, 2:107-117.