Proliferation and harvest of human mesenchymal stem cells using new thermoresponsive nanocomposite gels

Journal of Biomedical Materials Research - Part A

Volumn 101 A, Issue 2, 2013, Pages 537-546

  Kotobuki, Noriko ^a   Murata, Kazutaka ^a   Haraguchi, Kazutoshi ^a  

^a KAWAMURA INSTITUTE OF CHEMICAL RESEARCH   (Japan)

Author keywords

Differentiation; Human mesenchymal stem cells; Nanocomposite gel; Thermoresponsive cell harvest

Indexed keywords

CELL ATTACHMENTS; CELL CULTURE SUBSTRATE; CELL SHEET; CLAY CONTENT; CULTURE MEDIUM; EPITHELIAL CELLS; HECTORITES; HUMAN CELLS; HUMAN MESENCHYMAL STEM CELLS; IN-VITRO; INORGANIC CLAYS; MESENCHYMAL STEM CELL; MONOMER TYPES; N ,N-DIMETHYLACRYLAMIDE; NANOCOMPOSITE GELS; PROTEIN ADSORPTION; REGENERATIVE MEDICINE; STEM CELL DIFFERENTIATION; SUSPENSION CELL; SWELLING PROPERTIES; THERMO-RESPONSIVE; THERMOSENSITIVITIES; TRYPSINIZATION;

DIFFERENTIATION (CALCULUS); FLOWCHARTING; GELS; NANOCOMPOSITES; STEM CELLS; SURFACE PROPERTIES; SUSPENSIONS (FLUIDS); TISSUE ENGINEERING;

CELL CULTURE;

2 METHOXYETHYL ACRYLATE; COPOLYMER; HECTORITE; INORGANIC COMPOUND; N,N DIMETHYLACRYLAMIDE; NANOCOMPOSITE; POLYVINYLIDENE FLUORIDE; TRYPSIN; UNCLASSIFIED DRUG;

ADSORPTION; ARTICLE; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL JUNCTION; CELL PROLIFERATION; CELL SUSPENSION; CONTROLLED STUDY; CULTURE MEDIUM; EPITHELIUM CELL; FIBROBLAST; FILM; HEAT SENSITIVITY; HUMAN; HUMAN CELL; HYDROGEL; MESENCHYMAL STEM CELL; OSTEOBLAST; SURFACE PROPERTY; TEMPERATURE;

ADENOSINE TRIPHOSPHATE; ADSORPTION; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; CELL SHAPE; CULTURE MEDIA; GELS; HUMANS; MESENCHYMAL STROMAL CELLS; MICROSCOPY, ELECTRON, TRANSMISSION; NANOCOMPOSITES; POLYMERS; POLYVINYLS; TEMPERATURE;

EID: 84872684314     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.34355     Document Type: Article

References (26)

1. Erices A, Conget P, Minguell JJ Mesenchymal progenitor cells in human umbilical cord blood. Br J Hematol 2000; 109: 235-242.
2. Miernik KI, Karasinski J. Porcine uterus contains a population of mesenchymal stem cells. Reproduction 2012; 143: 203-209.
3. Kuroda R, Ishida K, Matsumoto T., Akisue T, Fujioka H, Mizuno K., Ohgushi H, Wakitani S, Kurosaka M. Treatment of a full-thickness articular cartilage defect in the femoral condyle of an athlete with autologous bone-marrow stromal cells. Osteoarthritis Cartilage 2007; 15: 226-231.
4. Nagaya N, Fujii T, Iwase T., Ohgushi H, Itoh T, Uematsu M., Yamagishi M, Mori H, Kangawa K., Kitamura S. Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis. Am J Physiol Heart Circ Physiol 2004; 287: H2670-H2676.
5. Kotobuki N, Hirose M, Takakura Y., Ohgushi H. Cultured autologous human cells for hard tissue regeneration: Preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs 2004; 28: 33-39.
6. Morishita T, Honoki K, Ohgushi H., Kotobuki N, Matsushima A, Takakura Y. Tissue engineering approach to the treatment of bone tumors: Three cases of cultured bone grafts derived from patients' mesenchymal stem cells. Artif Organs 2006; 30: 115-118.
7. Miyahara Y, Nagaya N, Kataoka M., Yanagawa B, Tanaka K, Hao H., Ishino K, Ishida H, Shimizu T., Kangawa K, Sano S, Okano T, Kitamura S, Mori H. Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med 2006; 12: 459-465.
8. Engler AJ, Sen S, Sweeney H.L., Discher DE Matrix elasticity directs stem cell lineage specification. Cell 2006; 126: 677-689.
9. Reilly GC, Engler AJ Intrinsic extracellular matrix properties regulate stem cell differentiation. J Biomech 2010; 43: 55-62.
10. Gilbert PM, Havenstrite KL, Magnusson K.E., Sacco A., Leonardi NA, Kraft P, Nguyen NK, Thrun S, Lutolf M.P., Blau HM Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Science 2010; 329: 1078-1081.
11. Haraguchi K, Takehisa T, Ebato M. Control of cell cultivation and cell sheet detachment on the surface of polymer/clay nanocomposite hydrogels. Biomacromolecules 2006; 7: 3267-3275.
12. Chang C-W, van Spreeuwel A, Zhang C, Varghese S. PEG/clay nanocomposite hydrogel: A mechanically robust tissue engineering scaffold. Soft Matter 2010; 6: 5157-5164.
13. Chen JP, Yang TF Applications of chitosan-based thermo-sensitive copolymers for harvesting living cell sheet. Appl Surf Sci 2008; 255: 297-300.
14. Kwon HJ, Yasuda K, Ohmiya Y., Honma K, Chen YM, Gong JP In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities. Acta Biomater 2010; 6: 494-501.
15. Haraguchi K, Masatoshi S, Kotobuki N., Murata K. Thermoresponsible cell adhesion/detachment on transparent nanocomposite films consisting of poly(2-methoxyethyl acrylate) and clay. J Biomater Sci Polym Ed 2011; 22: 2389-2406.
16. Haraguchi K, Takehisa T. Nanocomposite hydrogels: A unique organic-inorganic network structure with extraordinary mechanical, optical, and swelling/de-swelling properties. Adv Mater 2002; 14: 1120-1124.
17. Haraguchi K, Farnworth R, Ohbayashi A., Takehisa T. Compositional effects on mechanical properties of nanocomposite hydrogels composed of poly(N, N-dimethylacrylamide) and clay. Macromolecules 2003; 36: 5732-5741.
18. Haraguchi K, Ebato M, Takehisa T. Polymer-clay nanocomposites exhibiting abnormal necking phenomena accompanied by extremely large reversible elongations and excellent transparency. Adv Mater 2006; 18: 2250-2254.
19. Haraguchi K, Murata K, Takehisa T. Stimuli-responsive nanocomposite gels and soft nanocomposites consisting of inorganic clays and copolymers with different chemical affinities. Macromolecules 2011; 45: 385-391.
20. Tsutsumi S, Shimazu A, Miyazaki K., Pan H, Koike C, Yoshida E., Takagishi K, Kato Y. Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun 2001; 288: 413-419.
21. Kotobuki N, Hirose M, Funaoka H., Ohgushi H. Enhancement of in vitro osteoblastic potential after selective sorting of osteoblasts with high alkaline phosphatase activity from human osteoblastlike cells. Cell Transplant 2004; 13: 377-383.
22. Kotobuki N, Ioku K, Kawagoe D., Fujimori H, Goto S, Ohgushi H. Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics. Biomaterials 2005; 26: 779-785.
23. Haraguchi Y, Shimizu T, Yamato M., Okano T. Regenerative therapies using cell sheet-based tissue engineering for cardiac disease. Cardiol Res Pract 2011; 2011: 845170.
24. Ren HY, Zhu M, Haraguchi K. Characteristic swelling-deswelling of polymer/clay nanocomposite gels. Macromolecules 2011; 44: 8516-8526.
25. Saito T, Ohashi K, Utoh R., Shimizu H, Ise K, Suzuki H., Yamato M, Okano T, Gotoh M. Reversal of diabetes by the creation of neo-islet tissues into a subcutaneous site using islet cell sheets. Transplantation 2011; 92: 1231-1236.
26. Hirose M, Kwon OH, Yamato M, Kikuchi A., Okano T. Creation of designed shape cell sheets that are noninvasively harvested and moved onto another surface. Biomacromolecules 2000; 1: 377-381.