Soil zymography - A novel in situ method for mapping distribution of enzyme activity in soil

Soil Biology and Biochemistry

Volumn 58, Issue , 2013, Pages 275-280

  Spohn, Marie ^a   Carminati, Andrea ^a   Kuzyakov, Yakov ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Microbial activity; Rhizosphere; Soil enzyme; Spatial distribution; Zymography

Indexed keywords

AMYLASE ACTIVITY; CALIBRATION CURVES; COST-EFFICIENT; DIGITAL IMAGE ANALYSIS; ELECTROPHORESIS GELS; EMBEDDED SUBSTRATES; ENZYMATIC ACTIVITIES; ENZYME ASSAYS; HOTSPOTS; IN-SITU METHODS; MICROBIAL ACTIVITIES; RHIZOSPHERE; SOIL ENZYMES; SOIL STRUCTURE; SOIL SURFACES; TISSUE SECTIONS; TWO DIMENSIONAL DISTRIBUTION; ZYMOGRAPHY;

AMYLASES; ELECTROPHORESIS; ENZYME ACTIVITY; GELS; SPATIAL DISTRIBUTION; SUBSTRATES; TISSUE;

SOILS;

DIGITAL IMAGE; ENZYME ACTIVITY; GROWTH RATE; HOT SPOT; IN SITU MEASUREMENT; LEGUME; MICROBIAL ACTIVITY; RHIZOSPHERE; SOIL SURFACE; SPATIAL DISTRIBUTION;

LUPINUS POLYPHYLLUS;

EID: 84872665341     PISSN: 00380717     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.soilbio.2012.12.004     Document Type: Article

References (37)

1. Allison S.D., Jastrow J.D. Activities of extracellular enzymes in physically isolated fractions of restored grassland soils. Soil Biology and Biochemistry 2006, 38:3245-3256.
2. Becker J.M., Parkin T., Nakatsu C.H., Wilbur J.D., Konopka A. Bacterial activity, community structure, and centimeter-scale spatial heterogeneity in contaminated soil. Microbial Ecology 2006, 51:220-231.
3. Berner D., Marhan S., Keil D., Poll C., Schützenmeister A., Piepho H.P., Kandeler E. Land-use intensity modifies spatial distribution and function of soil microorganisms in grasslands. Pedobiologia 2011, 54:341-351.
4. Buroker K.M., Kennedy A.C., Kelley S.E. A Coomassie brilliant blue G250-based colorimetric assay for measuring activity of calpain and other proteases. Anal Biochem 1993, 208:387-392.
5. Carminati A., Moradi A.B., Vetterlein D., Vontobel P., Lehmann E., Weller U., Vogel H., Oswald S.E. Dynamics of soil water content in the rhizosphere. Plant and Soil 2010, 332:163-176.
6. Dick R., Kandeler E. Enzymes in Soil. Encyclopedia of Soils in the Environment 2005, Elsevier, Oxford, 448-456.
7. Enwall K., Throbäck I.N., Stenberg M., Söderström M., Hallin S. Soil resources influence spatial patterns of denitrifying communities at scales compatible with land management. Applied and Environmental Microbiology 2010, 76:2243-2250.
8. Ettema C.H., Wardle D.A. Spatial soil ecology. Trends in Ecology and Evology 2002, 17:177-183.
9. Gahoonia T.S., Nielsen N.E. A method to study rhizosphere processes in thin soil layers of different proximity to roots. Plant and Soil 1991, 135:143-146.
10. German D.P., Weintraub M.N., Stuart Grandy A., Lauber L.C., Rinkes Z.L., Allison S.D. Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies. Soil Biology and Biochemistry 2011, 43:1387-1397.
11. 2 oxidizing bacteria and serotypes at the millimeter scale along a soil transect. FEMS Microbiology Ecology 2000, 34:57-62.
12. 2 and different N supply in the rhizosphere of Phaseolus vulgaris L. Soil Biology and Biochemistry 2008, 40:1225-1234.
13. Hinsinger P., Bengough A., Vetterlein D., Young I. Rhizosphere: biophysics, biogeochemistry and ecological relevance. Plant and Soil 2009, 321:117-152.
14. Joanisse G.D., Bradley R.L., Preston C.M., Munson A.D. Soil enzyme inhibition by condensed litter tannins may drive ecosystem structure and processes: the case of Kalmia angustifolia. New Phytologist 2007, 175:535-546.
15. Kandeler E. Characterization of free and adsorbed phosphatases in soils. Biology and Fertility of Soils 1990, 9:199-202.
16. Kandeler E. Böden als Lebensraum. Bodenkunde und Standortlehre 2008, 261-284. UTB, Stuttgart. K. Stahr, E. Kandeler, L. Herrmann, T. Streck (Eds.).
17. Kandeler E., Marschner P., Tscherko D., Gahoonia T.S., Nielsen N.E. Microbial community composition and functional diversity in the rhizosphere of maize. Plant and Soil 2002, 238:301-312.
18. Kleiner D.E., Stetler-Stevenson W.G. Quantitative zymography: detection of pictogram quantities of gelatinases. Analytical Biochemistry 1994, 218:325-329.
19. Kuzyakov Y. Factors affecting rhizosphere priming effects. Journal of Plant Nutrition and Soil Science 2002, 165:382-396.
20. Kuzyakov Y., Raskatov A.V., Kaupenjohann M. Turnover and distribution of root exudates of Zea mays. Plant and Soil 2003, 254:317-327.
21. Li Y.T., Rouland C., Benedetti M., Li B., Pando A., Lavelle P., Dai J. Microbial biomass, enzyme and mineralization activity in relation to soil organic C, N and P turnover influenced by acid metal stress. Soil Biology and Biochemistry 2009, 41:969-977.
22. Manchenko G.P. Handbook of Detection of Enzymes on Electrophoretic Gels 2003, CRC Press, USA. second ed.
23. Marschner P., Gierson P.F., Rengel Z. Microbial community composition and functioning in the rhizosphere of three Banksia species in native woodland in Western Australia. Applied Soil Ecology 2005, 28:191-201.
24. Nannipieri P. Role of stabilised enzymes in microbial ecology and enzyme extraction from soil with potential applications in soil proteomics. Nucleic Acids and Proteins in Soil 2006, 75-94. Springer, Berlin. P. Nannipieri, K. Smalla (Eds.).
25. Nannipieri P., Kandeler E., Ruggiero P. Enzyme activities and microbiological and biochemical processes in soil. Enzymes in the Environment 2002, 1-33. Marcel Dekker, New York. R.G. Burns, R.P. Dick (Eds.).
26. Nemori R., Tachikawa T.A. Review of in situ zymography: method for localization of protease activities in a tissue. The Tissue Culture Engineering 1999, 25:29-32.
27. Nunan N., Wu K., Young M.N., Crawford J.W., Ritz K. In situ spatial patterns of soil bacterial populations, mapped at multiple scales, in an arable soil. Microbial Ecology 2002, 44:296-305.
28. 14C phosphor imaging. Journal of Plant Nutrition and Soil Science 2010, 174:12-19.
29. Ross D.J. Invertase and amylase activities in ryegrass and white clover plants and their relationships with activities in soils under pasture. Soil Biology and Biochemistry 1976, 8:351-356.
30. Rudolph N., Esser H.G., Carminati A., Moradi A.B., Hilger A., Kardjilov N., Nagl S., Oswald S.E. Dynamic oxygen mapping in the root zone by fluorescence dye imaging combined with neutron radiography. Journal of Soils and Sediments 2012, 12:63-74.
31. Sörensen J., Haubjerg Nicolaisen M., Ron E., Simonet P. Molecular tools in rhizosphere microbiology from single-cell to whole-community analysis. Plant and Soil 2009, 321:483-512.
32. Spohn, M., Ermak, A., Kuzyakov, Y. Microbial phosphorus mobilization is stimulated by root exudates: a 33P study. Soil Biology and Biochemistry, under review.
33. Tarafdar J.C., Jungk A. Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biology Fertility of Soils 1987, 3:199-204.
34. Wallenius K., Rita H., Mikkonen A., Lappi K., Lindström K., Hartikainen H., Raateland A., Niemi R.M. Effects of land use on the level, variation and spatial structure of soil enzyme activities and bacterial communities. Soil Biology and Biochemistry 2011, 43:1464-1473.
35. Wallenstein M.D., Weintraub M.N. Emerging tools for measuring and modeling the in situ activity of soil extracellular enzymes. Soil Biology and Biochemistry 2008, 40:2098-2106.
36. Wilkesman J., Kurz L. Protease analysis by zymography: a review on techniques and patents. Recent Patents on Biotechnology 2009, 2009:175-184.
37. Yoo Y.J., Hing J., Hatch R. Comparison of α-amylase activities from different assay methods. Biotechnology and Bioengineering 1987, 30:147-151.