Production of a de-novo designed antimicrobial peptide in Nicotiana benthamiana

Plant Molecular Biology

Volumn 81, Issue 3, 2013, Pages 259-272

  Zeitler, Benjamin ^a   Bernhard, Antonie ^a   Meyer, Helge ^a,b   Sattler, Michael ^a,b   Koop, Hans Ulrich ^c   Lindermayr, Christian ^a  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

Antimicrobial peptides; Designed peptide; Fusion protein; Molecular farming; Plant transformation; Tobacco mosaic virus

Indexed keywords

BACTERIA (MICROORGANISMS); NICOTIANA BENTHAMIANA; PSEUDOMONAS; PSEUDOMONAS CORRUGATA; PSEUDOMONAS SYRINGAE; TOBACCO MOSAIC VIRUS; XANTHOMONAS VESICATORIA;

EID: 84872608665     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-012-9996-9     Document Type: Article

References (73)

1. Ajikumar PK, Devaky KS (2001) Solid phase synthesis of hydrophobic difficult sequence peptides on BDDMA-PS support. J Pept Sci 7(12): 641-649. doi: 10. 1002/psc. 355.
2. Andreu D, Rivas L (1998) Animal antimicrobial peptides: an overview. Biopolymers 47(6): 415-433.
3. Asurmendi S, Berg RH, Smith TJ, Bendahmane M, Beachy RN (2007) Aggregation of TMV CP plays a role in CP functions and in coat-protein-mediated resistance. Virology 366(1): 98-106. doi: 10. 1016/j. virol. 2007. 03. 014.
4. Basaran P, Rodriguez-Cerezo E (2008) Plant molecular farming: opportunities and challenges. Crit Rev Biotechnol 28(3): 153-172. doi: 10. 1080/07388550802046624.
5. Bendahmane M, Fitchen JH, Zhang G, Beachy RN (1997) Studies of coat protein-mediated resistance to tobacco mosaic tobamovirus: correlation between assembly of mutant coat proteins and resistance. J Virol 71(10): 7942-7950.
6. Bendahmane M, Koo M, Karrer E, Beachy RN (1999) Display of epitopes on the surface of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions. J Mol Biol 290(1): 9-20. doi: 10. 1006/jmbi. 1999. 2860.
7. Boothe J, Nykiforuk C, Shen Y, Zaplachinski S, Szarka S, Kuhlman P, Murray E, Morck D, Moloney MM (2010) Seed-based expression systems for plant molecular farming. Plant Biotechnol J 8(5): 588-606. doi: 10. 1111/j. 1467-7652. 2010. 00511. x.
8. Brogden KA (2005) Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol 3(3): 238-250.
9. Castro MS, Fontes W (2005) Plant defense and antimicrobial peptides. Protein Pept Lett 12(1): 13-18.
10. Chapman SN (1998) Tobamovirus isolation and RNA extraction. Methods Mol Biol 81: 123-129. doi: 10. 1385/0-89603-385-6: 123.
11. Condron MM, Monien BH, Bitan G (2008) Synthesis and purification of highly hydrophobic peptides derived from the C-terminus of amyloid beta-protein. Open Biotechnol J 2(1): 87-93. doi: 10. 2174/1874070700802010087.
12. Crimmins DL, Mische SM, Denslow ND (2005) Chemical cleavage of proteins in solution. Curr Protoc Protein Sci Chapter 11: Unit 11 14. doi: 10. 1002/0471140864. ps1104s40.
13. Dathe M, Wieprecht T (1999) Structural features of helical antimicrobial peptides: their potential to modulate activity on model membranes and biological cells. Biochim Biophys Acta 1462(1-2): 71-87.
14. Durham AC (1972) Structures and roles of the polymorphic forms of tobacco mosaic virus protein. I. Sedimentation studies. J Mol Biol 67(2): 289-305.
15. Ehrenfeld N, Gonzalez A, Canon P, Medina C, Perez-Acle T, Arce-Johnson P (2008) Structure-function relationship between the tobamovirus TMV-Cg coat protein and the HR-like response. J Gen Virol 89(Pt 3): 809-817. doi: 10. 1099/vir. 0. 83355-0.
16. Eisenberg D (1984) Three-dimensional structure of membrane and surface proteins. Annu Rev Biochem 53: 595-623.
17. Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 7(2): 152-158. doi: 10. 1016/j. pbi. 2004. 01. 007.
18. Fitchen J, Beachy RN, Hein MB (1995) Plant virus expressing hybrid coat protein with added murine epitope elicits autoantibody response. Vaccine 13(12): 1051-1057. doi: 10264410X9500075C.
19. Fontana A, Gross E (1986) Fragmentation of polypeptides by chemical methods. In: Darbre A (ed) Practical protein chemistry: a handbook. Wiley, Chester, pp 67-120.
20. Fraenkel-Conrat H (1957) Degradation of tobacco mosaic virus with acetic acid. Virology 4(1): 1-4. doi: 10042-6822(57)90038-7.
21. Fraenkel-Conrat H, Williams RC (1955) Reconstitution of active tobacco mosaic virus from its inactive protein and nucleic acid components. Proc Natl Acad Sci U S A 41(10): 690-698.
22. Fraenkel-Conrat H, Singer B, Williams RC (1957) Infectivity of viral nucleic acid. Biochim Biophys Acta 25(1): 87-96.
23. Fritig B, Heitz T, Legrand M (1998) Antimicrobial proteins in induced plant defense. Curr Opin Immunol 10(1): 16-22.
24. Garcia-Olmedo F, Molina A, Alamillo JM, Rodriguez-Palenzuela P (1998) Plant defense peptides. Biopolymers 47(6): 479-491.
25. Gennaro R, Zanetti M (2000) Structural features and biological activities of the cathelicidin-derived antimicrobial peptides. Biopolymers 55(1): 31-49. doi: 10. 1002/1097-0282(2000)55: 1& 31: AID-BIP40> 3. 0. CO; 2-9.
26. Giddings G, Allison G, Brooks D, Carter A (2000) Transgenic plants as factories for biopharmaceuticals. Nat Biotechnol 18(11): 1151-1155. doi: 10. 1038/81132.
27. Gleba Y, Klimyuk V, Marillonnet S (2007) Viral vectors for the expression of proteins in plants. Curr Opin Biotechnol 18(2): 134-141. doi: 10. 1016/j. copbio. 2007. 03. 002.
28. Goelet P, Lomonossoff GP, Butler PJ, Akam ME, Gait MJ, Karn J (1982) Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci U S A 79(19): 5818-5822.
29. Goldstein DA, Thomas JA (2004) Biopharmaceuticals derived from genetically modified plants. QJM: Monthly Journal of the Association of Physicians 97(11): 705-716. doi: 10. 1093/qjmed/hch121.
30. Goodin MM, Zaitlin D, Naidu RA, Lommel SA (2008) Nicotiana benthamiana: its history and future as a model for plant-pathogen interactions. Mol Plant Microbe Interact 21(8): 1015-1026. doi: 10. 1094/MPMI-21-8-1015.
31. Gooding GV Jr, Hebert TT (1967) A simple technique for purification of tobacco mosaic virus in large quantities. Phytopathology 57(11): 1285.
32. Holt CA, Hodgson RA, Coker FA, Beachy RN, Nelson RS (1990) Characterization of the masked strain of tobacco mosaic virus: identification of the region responsible for symptom attenuation by analysis of an infectious cDNA clone. Mol Plant Microbe Interact 3(6): 417-423.
33. Horn ME, Woodard SL, Howard JA (2004) Plant molecular farming: systems and products. Plant Cell Rep 22(10): 711-720. doi: 10. 1007/s00299-004-0767-1.
34. Jervis L, Pierpoint WS (1989) Purification technologies for plant proteins. J Biotechnol 11: 161-198.
35. Kaiser R, Metzka L (1999) Enhancement of cyanogen bromide cleavage yields for methionyl-serine and methionyl-threonine peptide bonds. Anal Biochem 266(1): 1-8. doi: 10. 1006/abio. 1998. 2945.
36. Koprowski H, Yusibov V (2001) The green revolution: plants as heterologous expression vectors. Vaccine 19(17-19): 2735-2741.
37. Kusnadi AR, Nikolov ZL, Howard JA (1997) Production of recombinant proteins in transgenic plants: practical considerations. Biotechnol Bioeng 56(5): 473-484. doi: 10. 1002/(SICI)1097-0290(19971205)56: 5& 473: AID-BIT1> 3. 0. CO; 2-F.
38. Li Q, Li M, Jiang L, Zhang Q, Song R, Xu Z (2006) TMV recombinants encoding fused foreign transmembrane domains to the CP subunit caused local necrotic response on susceptible tobacco. Virology 348(2): 253-259. doi: 10. 1016/j. virol. 2005. 11. 013.
39. Lico C, Chen Q, Santi L (2008) Viral vectors for production of recombinant proteins in plants. J Cell Physiol 216(2): 366-377. doi: 10. 1002/jcp. 21423.
40. Lico C, Santi L, Twyman RM, Pezzotti M, Avesani L (2012) The use of plants for the production of therapeutic human peptides. Plant Cell Rep 31(3): 439-451. doi: 10. 1007/s00299-011-1215-7.
41. Lindbo JA (2007) TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Plant Physiol 145(4): 1232-1240. doi: 10. 1104/pp. 107. 106377.
42. Ma JK, Drake PM, Christou P (2003) The production of recombinant pharmaceutical proteins in plants. Nat Rev Genet 4(10): 794-805. doi: 10. 1038/nrg1177.
43. Ma JK, Barros E, Bock R, Christou P, Dale PJ, Dix PJ, Fischer R, Irwin J, Mahoney R, Pezzotti M, Schillberg S, Sparrow P, Stoger E, Twyman RM (2005) Molecular farming for new drugs and vaccines. Current perspectives on the production of pharmaceuticals in transgenic plants. EMBO Rep 6(7): 593-599. doi: 10. 1038/sj. embor. 7400470.
44. Marshall SH, Arenas G (2003) Antimicrobial peptides: a natural alternative to chemical antibiotics and a potential for applied biotechnology. Electron J Biotechnol 6(3): 271-284.
45. McCormick AA, Corbo TA, Wykoff-Clary S, Nguyen LV, Smith ML, Palmer KE, Pogue GP (2006) TMV-peptide fusion vaccines induce cell-mediated immune responses and tumor protection in two murine models. Vaccine 24(40-41): 6414-6423. doi: 10. 1016/j. vaccine. 2006. 06. 003.
46. Montesinos E (2007) Antimicrobial peptides and plant disease control. FEMS Microbiol Lett 270(1): 1-11. doi: 10. 1111/j. 1574-6968. 2007. 00683. x.
47. Nedoluzhko A, Douglas T (2001) Ordered association of tobacco mosaic virus in the presence of divalent metal ions. J Inorg Biochem 84(3-4): 233-240.
48. Nelson M, McClelland M (1992) Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes. Methods Enzymol 216: 279-303.
49. Nilsson MR, Nguyen LL, Raleigh DP (2001) Synthesis and purification of amyloidogenic peptides. Anal Biochem 288(1): 76-82. doi: 10. 1006/abio. 2000. 4887.
50. Obembe OO, Popoola JO, Leelavathi S, Reddy SV (2011) Advances in plant molecular farming. Biotechnol Adv 29(2): 210-222. doi: 10. 1016/j. biotechadv. 2010. 11. 004.
51. Palmer KE, Benko A, Doucette SA, Cameron TI, Foster T, Hanley KM, McCormick AA, McCulloch M, Pogue GP, Smith ML, Christensen ND (2006) Protection of rabbits against cutaneous papillomavirus infection using recombinant tobacco mosaic virus containing L2 capsid epitopes. Vaccine 24(26): 5516-5525. doi: 10. 1016/j. vaccine. 2006. 04. 058.
52. Papworth C, Braman J, Wright DA (1996) Site-directed mutagenesis in one day with > 80% efficiency. Strategies 9: 3-4.
53. Parish CL, Zaitlin M (1966) Defective tobacco mosaic virus strains: identification of the protein of strain PM1 in leaf homogenates. Virology 30(2): 297-302.
54. Pogue GP, Lindbo JA, Garger SJ, Fitzmaurice WP (2002) Making an ally from an enemy: plant virology and the new agriculture. Annu Rev Phytopathol 40: 45-74. doi: 10. 1146/annurev. phyto. 40. 021102. 150133.
55. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
56. Sampson WR, Patsiouras H, Ede NJ (1999) The synthesis of 'difficult' peptides using 2-hydroxy-4-methoxybenzyl or pseudoproline amino acid building blocks: a comparative study. J Pept Sci 5(9): 403-409. doi: 10. 1002/(SICI)1099-1387(199909)5: 9& 403: AID-PSC213> 3. 0. CO; 2-S.
57. Schägger H (2006) Tricine-SDS-PAGE. Nat Protoc 1(1): 16-22. doi: 10. 1038/nprot. 2006. 4.
58. Scheele RB, Lauffer MA (1967) Acid-base titrations of tobacco mosaic virus and tobacco mosaic virus protein. Biochemistry 6(10): 3076-3081.
59. Schillberg S, Fischer R, Emans N (2003) 'Molecular farming' of antibodies in plants. Naturwissenschaften 90(4): 145-155. doi: 10. 1007/s00114-002-0400-5.
60. Shinmyo A, Kato K (2010) Molecular farming: production of drugs and vaccines in higher plants. J Antibiot (Tokyo) 63(8): 431-433. doi: 10. 1038/ja. 2010. 63.
61. Simmaco M, Mignogna G, Barra D (1998) Antimicrobial peptides from amphibian skin: what do they tell us? Biopolymers 47(6): 435-450. doi: 10. 1002/(SICI)1097-0282(1998)47: 6& 435: AID-BIP3> 3. 0. CO; 2-8.
62. Smith BJ (1994) Chemical cleavage of proteins. Methods Mol Biol 32: 297-309. doi: 10. 1385/0-89603-268-X: 297.
63. Smith ML, Fitzmaurice WP, Turpen TH, Palmer KE (2009) Display of peptides on the surface of tobacco mosaic virus particles. Curr Top Microbiol Immunol 332: 13-31. doi: 10. 1007/978-3-540-70868-1_2.
64. Takamatsu N, Watanabe Y, Yanagi H, Meshi T, Shiba T, Okada Y (1990) Production of enkephalin in tobacco protoplasts using tobacco mosaic virus RNA vector. FEBS Lett 269(1): 73-76. doi: 0014-5793(90)81121-4.
65. Thomma BP, Cammue BP, Thevissen K (2002) Plant defensins. Planta 216(2): 193-202.
66. Turpen TH (1999) Tobacco mosaic virus and the virescence of biotechnology. Philos Trans R Soc Lond B Biol Sci 354(1383): 665-673. doi: 10. 1098/rstb. 1999. 0419.
67. Turpen TH, Reinl SJ, Charoenvit Y, Hoffman SL, Fallarme V, Grill LK (1995) Malarial epitopes expressed on the surface of recombinant tobacco mosaic virus. Biotechnology (N Y) 13(1): 53-57.
68. van 't Hof W, Veerman EC, Helmerhorst EJ, Amerongen AV (2001) Antimicrobial peptides: properties and applicability. Biol Chem 382(4): 597-619. doi: 10. 1515/BC. 2001. 072.
69. Werner S, Marillonnet S, Hause G, Klimyuk V, Gleba Y (2006) Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A. Proc Natl Acad Sci U S A 103(47): 17678-17683. doi: 10. 1073/pnas. 0608869103.
70. Wu L, Jiang L, Zhou Z, Fan J, Zhang Q, Zhu H, Han Q, Xu Z (2003) Expression of foot-and-mouth disease virus epitopes in tobacco by a tobacco mosaic virus-based vector. Vaccine 21(27-30): 4390-4398.
71. Yeaman MR, Yount NY (2003) Mechanisms of antimicrobial peptide action and resistance. Pharmacol Rev 55(1): 27-55. doi: 10. 1124/pr. 55. 1. 2.
72. Yuan JM, Hsiung LM, Gagnon J (1986) CNBr cleavage of the light chain of human complement factor I and alignment of the fragments. Biochem J 233(2): 339-345.
73. Zasloff M (2002) Antimicrobial peptides of multicellular organisms. Nature 415(6870): 389-395.