Fire blight: Applied genomic insights of the pathogen and host

Annual Review of Phytopathology

Volumn 50, Issue , 2012, Pages 475-494

  Malnoy, Mickael ^a   Martens, Stefan ^a   Norelli, John L ^b   Barny, Marie Anne ^c   Sundin, George W ^d   Smits, Theo H M ^e   Duffy, Brion ^e  

^a RESEARCH AND INNOVATION CENTRE   (Italy)

^b APPALACHIAN FRUIT RESEARCH STATION   (United States)

^c AGROPARISTECH   (France)

^d MICHIGAN STATE UNIVERSITY   (United States)

^e AGROSCOPE   (Switzerland)

Author keywords

Erwinia amylovora; evolutionary genomics; Malus; Pantoea; Pyrus; transcriptomics

Indexed keywords

BACTERIAL GENE; ERWINIA AMYLOVORA; GENETICS; GENOMICS; HOST PATHOGEN INTERACTION; MALUS; MICROBIOLOGY; PATHOGENICITY; PHYSIOLOGY; PLANT DISEASE; PLANT GENE; REVIEW; ROSACEAE; VIRULENCE;

ERWINIA AMYLOVORA; GENES, BACTERIAL; GENES, PLANT; GENOMICS; HOST-PATHOGEN INTERACTIONS; MALUS; PLANT DISEASES; ROSACEAE; VIRULENCE;

ENTEROBACTERIACEAE; ERWINIA AMYLOVORA; MALUS; MALUS X DOMESTICA; PANTOEA; PYRUS; ROSACEAE;

EID: 84872252485     PISSN: 00664286     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev-phyto-081211-172931     Document Type: Review

References (128)

1. Aldwinckle H, Malnoy M. 2009. Plant regeneration and transformation in the Rosaceae. Spec. Issue Transgen. Plant J. 1:1-39
2. Asselin JE, Bonasera JM, Kim JF, Oh CS, Beer SV. 2011. Eop1 from a Rubus strain of Erwinia amylovora functions as a host-range limiting factor. Phytopathology 101:935-44
3. Baldo A, Norelli JL, Farrell RE Jr, Bassett CL, Aldwinckle HS, Malnoy M. 2010. Identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora. BMC Plant Biol. 10:1
4. Barionovi D, Giorgi S, Stoeger AR, Ruppitsch W, Scortichini M. 2006. Characterization of Erwinia amylovora strains from different host plants using repetitive-sequences PCR analysis, and restriction fragment length polymorphism and short-sequence DNA repeats of plasmid pEA29. J. Appl. Microbiol. 100:1084-94
5. Bernard CS, Brunet YR, Gueguen E, Cascales E. 2010. Nooks and crannies in type VI secretion regulation. J. Bacteriol. 192:3850-60
6. Bocsanczy AM, Nissinen RM, Oh C-S, Beer SV. 2008. HrpN of Erwinia amylovora functions in the translocation of DspA/E into plant cells. Mol. Plant Pathol. 9:425-34
7. Bocsanczy AM, Phillips JG, Dardick CD, Korban SS, Bassett CL, et al. 2009. Analysis of apple (Malus) responses to bacterial pathogens using an oligo microarray. Phytopathology 99:S514
8. Bocsanczy AM, Schneider DJ, Declerck GA, Cartinhour S, Beer SV. 2012. HopX1ea (Eop3) in Erwinia amylovora functions as an avirulence gene in apple and is regulated by HrpL. J. Bacteriol. 194:553-60
9. Bonasera JM, Kim JF, Beer SV. 2006. PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora. BMC Plant Biol. 6:23
10. Bonaterra A, Camps J,Montesinos E. 2005. Osmotically induced trehalose and glycine betaine accumulation improves tolerance to dessication, survival and efficacy of the postharvest biocontrol agent Pantoea agglomerans EPS125. FEMS Microbiol. Lett. 250:1-8
11. Borejsza-Wysocka E, Malnoy M, Meng X, Bonasera JM, Nissinen RM, et al. 2006. The fire blight resistance of apple clones in which DspE-interacting proteins are silenced. Acta Hortic. 704:509-13
12. Born Y, Fiesler L, Marazzi J, Lurz R, Duffy B, Loessner MJ. 2011. Novel virulent and broad host range Erwinia amylovora bacteriophages reveal a high degree of mosaicism and relationship to Enterobacteriacea phages. Appl. Environ. Microbiol. 77:5945-54
13. Boureau T, El Maarouf-Bouteau H, Garnier A, Brisset MN, Perino C, et al. 2006. DspA/E, a type III effector essential for Erwinia amylovora pathogenicity and growth in planta, induces cell death in host apple and nonhost tobacco plants. Mol. Plant-Microbe Interact. 19:16-24
14. BoureauT, Siamer S, Perino C, Gaubert S, Patrit O, et al. 2011. The HrpN effector of Erwinia amylovora, which is involved in type III translocation, contributes directly or indirectly to callose elicitation on apple leaves. Mol. Plant-Microbe Interact. 24:577-84
15. Broggini GAL, Duffy B, Holliger E, Sch?arer H-J, Gessler C, Patocchi A. 2005. Detection of the fire blight biocontrol agent Bacillus subtilis BD170 (BioproR) in a Swiss apple orchard. Eur. J. Plant Pathol. 111:93-100
16. Calenge F, Drouet D, Denance C, Van de WegWE, Brisset MN, et al. 2005. Identification of a major QTL together with several minor additive or epistatic QTLs for resistance to fire blight in apple in two related progenies. Theor. Appl. Genet. 111:128-35
17. Coplin DL, Majerczak DR, Bugert P, Geider K. 1996. Nucleotide sequence analysis of the Erwinia stewartii cps gene cluster for synthesis of stewartan and comparison to the Erwinia amylovora ams cluster for synthesis of amylovoran. Acta Hortic. 411:251-57
18. DebRoy S, Thilmony R, Kwack YB, Nomura K, He SY. 2004. A family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants. Proc. Natl. Acad. Sci. USA 101:9927-32
19. Djennane S, Cesbron C, Sourice S, Cournol R, Dupuis F, et al. 2011. Iron homeostasis and fire blight susceptibility in transgenic pear plants overexpressing a pea ferritin gene. Plant Sci. 180:694-701
20. Dondini L, Pierantoni L,Gaiotti F, Chiodini R, Tartarini S, et al. 2004. Identifying QTLs for fire blight resistance via a European pear (Pyrus communis L.) genetic linkage map. Mol. Breed. 14:407-18
21. Dreo T, Ravnikar M, Frey JE, Smits THM, Duffy B. 2011. In silico analysis of variable number of tandem repeats in Erwinia amylovora genomes. Acta Hortic. 896:115-18
22. Duffy B, Sch?arer H-J, Vogelsanger J, Schoch B, Holliger E. 2005. Regulatory measures against Erwinia amylovora in Switzerland. EPPO Bull. 35:239-44
23. Durel C-E, Denance C, Brisset MN. 2009. Two distinct major QTL for resistance to fire blight colocalize on linkage group 12 in apple genotypes "Evereste" and Malus floribunda clone 821. Genome 52:139-47
24. Engelhardt S, Lee J, Gaebler Y, Kemmerling B, Haapalainen M-L, et al. 2009. Separable roles of the Pseudomonas syringae pv. phaseolicola accessory protein HrpZ1 in ion-conducting pore formation and activation of plant immunity. Plant J. 57:706-17
25. Fahrentrapp J, Broggini GAL, Kellerhals M, Peil A, Richter K, et al. 2012. Malus × robusta 5 fire blight candidate resistance gene coding for a CC-NBS-LRR. PloS ONE. In press
26. Feistner GJ, Ishimaru C. 1996. Proferrioxamine profiles of Erwinia herbicola and related bacteria. BioMetals 9:337-44
27. Flachowsky H, Halbwirth H, Treutter D, Richter K, Hanke MV, et al. 2012. Silencing of flavanone-3-hydroxylase in apple (Malus × domestica Borkh.) leads to accumulation of flavanones, but not to reduced fire blight susceptibility. Plant Physiol. Biochem. 51:18-25
28. Flachowsky H, Le Roux PM, Peil A, Patocchi A, Richter K, Hanke MV. 2011. Application of a highspeed breeding technology to apple (Malus × domestica) based on transgenic early flowering plants and marker-assisted selection. New Phytol. 192:364-67
29. Flachowsky H, Szankowski I, Fischer TC, Richter K, Peil A, et al. 2010. Transgenic apple plants overexpressing the Lc gene of maize show an altered growth habit and increased resistance to apple scab and fire blight. Planta 231:623-35
30. Foster GC, McGhee GC, Jones AL, Sundin GW. 2004. Nucleotide sequences, genetic organization, and distribution of pEU30 and pEL60 from Erwinia amylovora. Appl. Environ. Microbiol. 70:7539-44
31. Franc?es J, Bonaterra A, Moreno MC, Cabrefiga J, Badosa E,Montesinos E. 2006. Pathogen aggressiveness and postharvest biocontrol efficiency in Pantoea agglomerans. Postharvest Biol. Technol. 39:299-307
32. GaletskiyD, Susnea I, Reiser V, Adamska I, PrzybylskiM. 2008. Structure and dynamics of photosystem II light-harvesting complex revealed by high-resolution FTICR mass spectrometric proteome analysis. J. Am. Soc. Mass Spectrom. 19:1004-13
33. Gardiner SE, Norelli JL, de Silva N, Fazio G, Peil A, et al. 2012. Candidate gene markers associated with quantitative trait loci for fire blight resistance in Malus "robusta 5" accessions. BMC Genet. 13(1):25
34. Gasic K, Gonzalez DO, Thimmapuram J, et al. 2009. Comparative analysis and functional annotation of a large expressed sequence tag collection of apple. Plant Genome 2:23-38
35. Gaudriault S, Paulin JP, Barny MA. 2002. The DspB/F protein of Erwinia amylovora is a type III secretion chaperone ensuring efficient intrabacterial production of the Hrp-secreted DspA/E pathogenicity factor. Mol. Plant Pathol. 3:313-20
36. Griffith CS, Sutton TB, Peterson PD. 2003. Fire Blight: The Foundation of Phytobacteriology. St. Paul MN: APS Press. 144 pp.
37. Hubert DA, He Y, McNulty BC, Tornero P, Dangl JL. 2009. Specific Arabidopsis HSP90.2 alleles recapitulate RAR1 cochaperone function in plant NB-LRR disease resistance protein regulation. Proc. Natl. Acad. Sci. USA 106:9556-63
38. Ishimaru CA, Klos EJ, Brubaker RR. 1988. Multiple antibiotic productions by Erwinia herbicola. Phytopathology 78:746-50
39. Jang YS, Sohn SI, Wang MH. 2006. The hrpN gene of Erwinia amylovora stimulates tobacco growth and enhances resistance to Botrytis cinerea. Planta 223:449-56
40. Jensen PJ, Halbrendt N, Fazio G, Makalowska I, Altman N, et al. 2012. Rootstock-regulated gene expression patterns associated with fire blight resistance in apple. BMC Genomics 9:13
41. Jensen PJ,Makalowska I, Altman N, Fazio G, Praul C, et al. 2010. Rootstock-regulated gene expression patterns in apple tree scions. Tree Genet. Genomes 6:57-72
42. Johnson KB, Stockwell VO. 1998. Management of fire blight: a case study in microbial ecology. in Annu. Rev. Phytopathol. 36:227-48
43. Kamber T, Lansdell TA, Stockwell VO, Ishimaru CA, Smits THM, Duffy B. 2012. Characterization of the antibacterial peptide herbicolin I biosythetic operon in Pantoea vagans biocontrol strain C9-1, and prevalence in Pantoea species. Appl. Environ. Microbiol. 78:4412-19
44. Kamber T, Smits THM, Rezzonico F, Duffy B. 2012. Genomics and current genetic understanding of Erwinia amylovora and the fire blight antagonist Pantoea vagans. Trees Struct. Funct. 26:227-38
45. Khajanchi BK, Sha J, Kozlova EV, Erova TE, SuarezG, et al. 2009. N-acylhomoserine lactones involved in quorum sensing control type VI secretion system, biofilm formation, protease production, and in vivo virulence from a clinical isolate of Aeromonas hydrophila. Microbiology 155:3518-31
46. Khan MA, Duffy B, Gessler C, Patocchi A. 2006. QTL mapping of fire blight resistance in apple. Mol. Breed. 17:299-306
47. Khan MA, Zhao YF, Korban SS. 2012. Molecular mechanisms of pathogenesis and resistance to the bacterial pathogen Erwinia amylovora, causal agent of fire blight disease in Rosaceae. PlantMol. Biol. Rep. 30:247-60
48. Khan MA, Zhao YF, Korban SS. 2012. A high-throughput apple SNP genotyping platform using the GoldenGateTM assay. Gene 494:196-201
49. Koczan JM,McGrath MJ, Zhao Y, SundinGW. 2009. Contribution of Erwinia amylovora exopolysaccharides amylovoran and levan to biofilm formation: implications in pathogenicity. Phytopathology 99:1237-44
50. KubeM,MigdollAM,Gehring I,HeitmannK,Mayer Y, et al. 2010.Genomecomparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae. BMC Genomics 11:393
51. Kube M, Migdoll AM,Müller I, Kuhl H, Beck A, et al. 2008. The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia. Environ. Microbiol. 10:2211-22
52. Le Roux P-M, Flachowsky H, Hanke M-V, Gessler C, Patocchi A. 2011. Use of a transgenic early flowering approach in apple (Malus × domestica Borkh.) to introgress fire blight resistance from "Evereste." Mol. Breed. doi: 10.1007/s11032-011-9669-4
53. Le Roux PMF, Khan MA, Broggini GAL,Duffy B, Gessler C, Patocchi A. 2010. Mapping of quantitative trait loci for fire blight resistance in the apple cultivars "Florina" and "Nova Easygro." Genome 53:710-22
54. Llop P, BarbéS,L? opezMM.2012. Functions and origin of plasmids in Erwinia species that are pathogenic to or epiphytically associated with pome fruit trees. Trees Struct. Funct. 26:31-46
55. Llop P, Cabrefiga J, Smits THM, Dreo T, Barbé S, et al. 2011. Complete sequence, biogeography, and role in aggressiveness of a novel Erwinia amylovora plasmid pEI70. PLoS ONE 6(12):e28651
56. Llop P, Donat V, RodrguezM, Cabrefiga J, Ruz L, et al. 2006. An indigenous virulent strain of Erwinia amylovora lacking the ubiquitous plasmid pEA29. Phytopathology 96:900-7
57. Loh J, Pierson EA, Pierson LS III, Stacey G, Chatterjee A. 2002. Quorum sensing in plant-associated bacteria. Curr. Opin. Plant Biol. 5:285-90
58. López MM, Roselló M, Llop P, Ferrer S, Christen R, Gardan L. 2011. Erwinia piriflorinigrans sp. nov., a novel pathogen that causes necrosis of pear blossoms. Int. J. Syst. Evol. Microbiol. 61:561-67
59. Malnoy M, Boresjza-Wysocka EW, Norelli JL, Flaishman M, Gidoni D, Aldwinckle HS. 2010. Genetic transformation of apple (Malus × domestica) without use of a selectable marker gene. Tree Genet. Genomes 6:423-33
60. Malnoy M, Jin Q, Borejsza-Wysocka E, He SY, Aldwinckle HS. 2007. Overexpression of the apple MpNPR1 gene causes increased disease resistance in Malus × domestica. Mol. Plant-Microbe Interact. 20:1568-80
61. Malnoy M, Venisse JS, Brisset MN, Chevreau E. 2003. Expression of bovine lactoferrin cDNA confers resistance to Erwinia amylovora in transgenic pear. Mol. Breed. 12:231-44
62. Malnoy M, Venisse JS, Chevreau E. 2005. Expression of a bacterial effector, harpin N, causes increased resistance to fire blight in Pyrus communis. Tree Genet. Genomes 1:41-49
63. Mann RA, Blom J, Bühlmann A, Plummer KM, Beer SV, et al. 2012. Comparative analysis of the Hrp pathogenicity island of Rubus-and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element. Gene. 504:6-12
64. Maxson-Stein K, McGhee GC, Smith JJ, Jones AL, Sundin GW. 2003. Genetic analysis of a pathogenic Erwinia sp. isolated from pear in Japan. Phytopathology 93:1393-99
65. McGhee GC, Jones AL. 2000. Complete nucleotide sequence of ubiquitous plasmid pEA29 from Erwinia amylovora strain Ea88: gene organization and intraspecies variation. Appl. Environ.Microbiol. 66:4897-907
66. McGhee GC, Schnabel EL, Maxson-Stein K, JonesB, Stromberg VK, et al. 2002. Relatedness of chromosomal and plasmidDNAs of Erwinia pyrifoliae and Erwinia amylovora. Appl. Environ.Microbiol. 68:6182-92
67. McGhee GC, Sundin GW. 2011. Evaluation of kasugamycin for fire blight management, effect on nontarget bacteria, and assesment of kasugamycin resistance potential in Erwinia amylovora. Phytopathology 101:192-204
68. McManus PS, Stockwell VO, Sundin GW, Jones AL. 2002. Antibiotic use in plant agriculture. Annu. Rev. Phytopathol. 40:443-65
69. McNally RR, Toth IK, Cock PJA, Pritchard L, Hedley PE, et al. 2012. Genetic characterization of the HrpL regulon of the fire blight pathogen Erwinia amylovora reveals novel virulence factors. Mol. Plant Pathol. 13:160-73
70. Meng XD, Bonasera JM, Kim JF, NissinenRM, Beer SV. 2006. Apple proteins that interact with DspA/E, a pathogenicity effector of Erwinia amylovora, the fire blight pathogen. Mol. Plant-Microbe Interact. 19:53-61
71. Metzger M, Bellemann P, Bugert P, Geider K. 1994. Genetics of galactose metabolism of Erwinia amylovora and its influence on polysaccharide synthesis and virulence of the fire blight pathogen. J. Bacteriol. 176:450-59
72. Milecevicova R, Gosch R, Halbwirth H, Stich K, Hanke MV, et al. 2011. Erwinia amylovora-induced defense mechanisms of two apple species that differ in susceptibility to fire blight. Plant Sci. 179:60-67
73. Momol MT, Norelli JL, Piccioni DE, Momol EA, Gustafson HL, et al. 1998. Internal movement of Erwinia amylovora through symptomless apple scion tissues into the rootstock. Plant Dis. 70:1017-19
74. Nissinen RM, Ytterberg AJ, Bogdanove AJ, van Wijk KJ, Beer SV. 2007. Analyses of the secretomes of Erwinia amylovora and selected hrp mutants reveal novel type III secreted proteins and an effect of HrpJ on extracellular harpin levels. Mol. Plant Pathol. 8:55-67
75. Norelli JL, Aldwinckle HS, Beer SV. 1986. Differential susceptibility of Malus spp. cultivars robusta 5, Novole, and Ottawa 523 to Erwinia amylovora. Plant Dis. 70:1017-19
76. Norelli JL, Farrell RE, Bassett CL, Baldo AM, Lalli DA, et al. 2009. Rapid transcriptional response of apple to fire blight disease revealed by cDNA suppression subtractive hybridization analysis. Tree Genet. Genomes 5:27-40
77. Norelli JL, Jones AL, Aldwinckle HS. 2003. Fire blight management in the twenty-first century: using new technologies that enhance host resistance in apple. Plant Dis. 87:756-65
78. Oh C-S, Beer SV. 2005. Molecular genetics of Erwinia amylovora involved in the development of fire blight. FEMS Microbiol. Lett. 253:185-92
79. Oh C-S, Beer SV. 2007. AtHIPM, an ortholog of the apple HrpN-interacting protein, is a negative regulator of plant growth and mediates the growth-enhancing effect ofHrpN in Arabidopsis. Plant Physiol. 145:426-36
80. Oh C-S, Carpenter CD, Hayes ML, Beer SV. 2010. Secretion and translocation signals and DspB/Fbinding domains in the type III effector DspA/E of Erwinia amylovora. Microbiology 156:1211-20
81. Oh C-S, Martin GB, Beer SV. 2007. DspA/E, a type III effector of Erwinia amylovora, is required for early rapid growth in Nicotiana benthamiana and causesNbSGT1-dependent cell death. Mol. Plant Pathol. 8:255-65
82. Park DH, Thapa SP, Choi B-S, Kim W-S, Hur JH, et al. 2011. Complete genome sequence of Japanese Erwinia strain Ejp617, a bacterial shoot blight pathogen of pear. J. Bacteriol. 193:586-87
83. Parravicini G, Gessler C, Denancé C, Lasserre-Zuber P, Vergne E, et al. 2011. Identification of serine/threonine kinase and nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes in the fire blight resistance quantitative trait locus of apple cultivar "Evereste." Mol. Plant Pathol. 12:493-505
84. Parsot C, Hamiaux C, Page AL. 2003. The various and varying roles of specific chaperones in type III secretion systems. Curr. Opin. Microbiol. 6:7-14
85. Paternoster T, Défago G, Duffy B, Gessler C, Pertot I. 2010. Selection of a biocontrol agent based on a potential mechanism of action: degradation of nicotinic acid, a growth factor essential for Erwinia amylovora. Int. Microbiol. 13:195-206
86. Peace C, Norelli JL. 2009. Genomics approaches to crop improvement in the Rosaceae. In Genetics and Genomics of Rosaceae, ed. K Folta, S Gardiner, pp. 55-70. New York: Springer
87. Peil A, Flachowsky H, Hanke M-V, Richter K, Rode J. 2011. Inoculation of Malus × robusta 5 progeny with a strain breaking resistance to fire blight reveals a minor QTL on LG5. Acta Hortic. 986:357-62
88. Peil A, Garcia-Libreros T, Richter K, Trognitz FC, Trognitz B, et al. 2007. Strong evidence for a fire blight resistance gene of Malus robusta located on linkage group 3. Plant Breed. 126:470-75
89. Perino C, Gaudriault S, Vian B, Barny MA. 1999. Visualization of harpin secretion in planta during infection of apple seedlings by Erwinia amylovora. Cell. Microbiol. 1:131-41
90. Powney R, Smits THM, Sawbridge T, Frey B, Blom J, et al. 2011. Genome sequence of an Erwinia amylovora strain with restricted pathogenicity to Rubus plants. J. Bacteriol. 193:785-86
91. Pusey PL, Curry EA. 2004. Temperature and pomaceous flower age related to colonization by Erwinia amylovora and antagonists. Phytopathology 94:901-11
92. Pusey PL, Stockwell VO, Reardon C, Smits THM, Duffy B. 2011. Antibiotic production by Pantoea agglomerans biocontrol strain E325 and activity against Erwinia amylovora on apple flower stigmas. Phytopathology 101:1234-41
93. Reboutier D, Frankart C, Briand J, Biligui B, Laroche S, et al. 2007. The HrpNea harpin from Erwinia amylovora triggers differential responses on the nonhost Arabidopsis thaliana cells and on the host apple cells. Mol. Plant-Microbe Interact. 20:94-100
94. Rezzonico F, Duffy B. 2007. The role of luxS in the fire blight pathogen Erwinia amylovora is limited to metabolism and does not involve quorum sensing. Mol. Plant-Microbe Interact. 20:1284-97
95. Rezzonico F, Duffy B. 2008. Lack of genomic evidence of AI-2 receptors suggests a non-quorum sensing role for luxS in most bacteria. BMC Microbiol. 8:154
96. Rezzonico F, Smits THM, Duffy B. 2011. Diversity, evolution, and functionality of clustered regularly interspaced short palindromic repeat (CRISPR) regions in the fire blight pathogen Erwinia amylovora. Appl. Environ. Microbiol. 77:3819-29
97. Rezzonico F, Smits THM, Montesinos E, Frey JE, Duffy B. 2009. Genotypic comparison of Pantoea agglomerans plant and clinical strains. BMC Microbiol. 9:204
98. Rezzonico F, Vogel G, Duffy B, Tonolla M. 2010. Application of whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification and clustering analysis of Pantoea species. Appl. Environ. Microbiol. 76:4497-509
99. Sarowar S, Zhao Y, Soria-Guerra RE, Ali S, Zheng D, et al. 2011. Expression profiles of differentially regulated genes during the early stages of apple flower infection with Erwinia amylovora. J. Exp. Bot. 62:4851-61
100. Sebaihia M, Bocsanczy AM, Biehl BS, Quail MA, Perna NT, et al. 2010. Complete genome sequence of the plant pathogen Erwinia amylovora strain ATCC 49946. J. Bacteriol. 192:2020-21
101. Singh DK, Maximova SN, Jensen PJ, Lehman BL, Ngugi HK, McNellis TW. 2010. FIBRILLIN4 is required for plastoglobule development and stress resistance in apple and Arabidopsis. Plant Physiol. 154:1281-93
102. Smits THM, Duffy B. 2011. Genomics of iron acquisition in the plant pathogen Erwinia amylovora: insights in the biosynthetic pathway of the siderophore desferrioxamine E. Arch. Microbiol. 193:693-99
103. Smits THM, Jaenicke S, Rezzonico F, Kamber T, Goesmann A, et al. 2010. Complete genome sequence of the fire blight pathogen Erwinia pyrifoliae DSM 12163T and comparative genomic insights into plant pathogenicity. BMC Genomics 11:2
104. Smits THM, Rezzonico F, Blom J, Frey JE, López MM, Duffy B. 2012. Comparative analysis of the genome of Erwinia piriflorinigrans CFBP 5888T reveals its pathogenic potential and its phylogenetic position within the Erwinia species pathogenic to Spiraeoideae host plants. BMC Genomics. Submitted
105. Smits THM, Rezzonico F, Duffy B. 2011. Evolutionary insights from Erwinia amylovora genomics. J. Biotechnol. 155:34-39
106. Smits THM, Rezzonico F, Kamber T, Blom J, Goesmann A, et al. 2010. Complete genome sequence of the fire blight pathogen Erwinia amylovora CFBP 1430 and comparison to other Erwinia spp. Mol. Plant-Microbe Interact. 23:384-93
107. Smits THM, Rezzonico F, Kamber T, Goesmann A, Ishimaru CA, et al. 2011. Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1. PLoS ONE 6:e22247
108. Smits THM, Rezzonico F, Kamber T, Goesmann A, Ishimaru CA, et al. 2010. The genome sequence of the biocontrol agent Pantoea vagans strain C9-1. J. Bacteriol. 192:6486-87
109. Smits THM, Rezzonico F, Pelludat C, Goesmann A, Frey JE, Duffy B. 2010. Genomic and phenotypic characterization of a non-pigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530 kb megaplasmid pPag3. FEMS Microbiol. Lett. 308:48-54
110. Song X, Fan H, Wei Z-M. 2002. Receptors for hypersensitive response elicitors and uses thereof. U.S. Patent No. 20020007501
111. Soria-Guerra RE, Rosales-Mendoza SR, Gasic K, Wisniewski ME, Band M, Korban SS. 2011. Gene expression is highly regulated in early developing fruit of apple. Plant Mol. Biol. Rep. 29:885-97
112. Thomson SV. 1986. The role of the stigma in fire blight infections. Phytopathology 76:476-82
113. Treutter D. 2006. Significance of flavonoids in plant resistance and enhancement of their biosynthesis. Plant Biol. 7:581-91
114. Triplett LR, Melotto M, Sundin GW. 2009. Functional analysis of the N terminus of the Erwinia amylovora secreted effector DspA/E reveals features required for secretion, translocation, and binding to the chaperone DspB/F. Mol. Plant-Microbe Interact. 22:1282-92
115. Triplett LR,Wedemeyer WJ, Sundin GW. 2010. Homology-based modeling of the Erwinia amylovora type III secretion chaperone DspF used to identify amino acids required for virulence and interaction with the effector DspE. Res. Microbiol. 161:613-18
116. Truman W, de Zabala MT, Grant M. 2006. Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J. 46:14-33
117. van der Zwet T, Orolaza-Halbrendt N, ZellerW. 2011. Fire Blight: History, Biology and Management. St Paul, MN: APS Press. 420 pp.
118. vanneste JL. 2000. What is fire blight? Who is Erwinia amylovora? How to control it? In Fire Blight, ed. J. vanneste, pp. 1-7. New York: CABI
119. Velasco R, Zharkikh A, Affourtit J, Dhingra A, Cestaro A, et al. 2010. The genome emergence of the domesticated apple (Malus × domestica Borkh.). Nat. Genet. 42:833-41
120. Venisse JS,BarnyMA,Paulin JP,BrissetMN. 2003. Involvement of three pathogenicity factors of Erwinia amylovora in the oxidative stress associated with compatible interaction in pear. FEBS Lett. 537:198-202
121. Venisse JS, Gullner G, Brisset MN. 2001. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora. Plant Physiol. 125:2164-72
122. Venisse JS, Malnoy M, Faize M, Brisset MN. 2002. Modulation of plant defense responses of Malus spp. during compatible and incompatible interaction with Erwinia amylovora. Mol. Plant-Microbe Interact. 15:1204-12
123. Wang D, Qi M, Calla B, Korban SS, Clough SJ, et al. 2012. Genome-wide identification of genes regulated by the Rcs phosphorelay system in Erwinia amylovora. Mol. Plant-Microbe Interact. 25:6-17
124. Yoder KS, Miller SS, Byers RE. 1999. Suppression of fireblight in apple shoots by prohexadione-calcium following experimental and natural inoculation. HortScience 34:1202-4
125. ZhangW,Xu S, Li J, ShenX,Wang Y, Yuan Z. 2011. Modulation of a thermoregulated typeVI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch. Microbiol. 193:351-63
126. Zhao Y, Sundin GW,WangD. 2009. Construction and analysis of pathogenicity island deletionmutants of Erwinia amylovora. Can. J. Microbiol. 55:457-64
127. Zhao YF, Blumer SE, Sundin GW. 2005. Identification of Erwinia amylovora genes induced during infection of immature pear tissue. J. Bacteriol. 187:8088-103
128. Zhao YF, He SY, Sundin GW. 2006. The Erwinia amylovora avrRpt2EA gene contributes to virulence on pear and AvrRpt2EA is recognized by Arabidopsis RPS2 when expressed in Pseudomonas syringae. Mol. Plant-Microbe Interact. 19:644-54