An Efficient In-gel Digestion Protocol for Mass Spectral Analysis by MALDI-TOF-MS and MS/MS and Its Use for Proteomic Analysis of Vigna mungo Leaves

Plant Molecular Biology Reporter

Volumn 31, Issue 1, 2013, Pages 47-54

  Kundu, Subrata ^a   Chakraborty, Dipjyoti ^a,b   Das, Kalipada ^a   Pal, Amita ^a  

^a BOSE INSTITUTE   (India)

^b BANASTHALI UNIVERSITY   (India)

Author keywords

In gel digestion; MALDI TOF MS and MS MS; Plant proteomics; Vigna mungo

Indexed keywords

VIGNA MUNGO;

EID: 84872179614     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/s11105-012-0475-x     Document Type: Article

References (21)

1. Aghaei K, Ali AE, Setsuko K (2008) Proteome analysis of potato under salt stress. J Proteome Res 7: 4858-4868.
2. Basak J, Kundagrami S, Ghosh TK, Pal A (2005) Development of yellow mosaic virus (YMV) resistance linked DNA marker in Vigna mungo from populations segregating for YMV-reaction. Mol Breed 14: 375-383.
3. Basha SM, Roberts RM (1981) The glycoproteins of plant seeds: analysis by two-dimensional polyacrylamide gel electrophoresis and by their lectin-binding properties. Plant Physiol 67: 936-939.
4. Basha SM, Katam R, Vasanthaiah H, Matta F (2009) Proteome analysis of muscadine grape leaves. Int J Wine Res 1: 1-13.
5. Bradford MM (1976) A rapid and sensitive method for quantification of proteins utilizing the principle of protein dye binding. Anal Biochem 72: 248-254.
6. Ding C, You J, Liu Z, Rehmani IA, Wang S, Li G, Wang O, Ding Y (2011) Proteomic analysis of low nitrogen stress-responsive proteins in roots of rice. Plant Mol Biol Rep 29: 618-625.
7. Emanuelsson O, Brunak S, Heijne G, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc 2: 953-971.
8. Granvogl B, Ploscher M, Eichacker LA (2007) Sample preparation by in-gel digestion for mass spectrometry-based proteomics. Anal Bioanal Chem 389: 991-1002.
9. Hashiguchi A, Sakata K, Komatsu S (2009) Proteome analysis of early-stage soybean seedlings under flooding stress. J Proteome Res 8: 2058-2069.
10. Havlis J, Thomas H, Sebela M, Shevchenko A (2003) Fast-response proteomics by accelerated in-gel digestion of proteins. Anal Chem 75: 1300-1306.
11. Isaacson T, Damasceno CMB, Saravanan RS, He Y, Catala C, Saladie M, Rose JKC (2006) Sample extraction techniques for enhanced proteomic analysis of plant tissues. Nat Protoc 1: 769-774.
12. Kong F, Mao S, Jiang J, Wang J, Fang X, Wang Y (2011) Proteomic changes in newly synthesized Brassica napus allotetraploids and their early generations. Plant Mol Biol Rep 29: 927-935.
13. Kumarathasan P, Mohottalage S, Goegan P, Vincent R (2005) An optimized protein in-gel digest method for reliable proteome characterization by MALDI-TOF-MS analysis. Anal Biochem 346: 85-89.
14. Kundagrami S, Basak J, Maiti S, Kundu A, Das B, Ghosh TK, Pal A (2009) Agronomic, genetic and molecular characterization of MYMIV tolerant mutant lines of Vigna mungo. Int J Plant Breed Genet 3: 1-10.
15. Kundu S, Chakraborty D, Pal A (2011) Proteomic analysis of salicylic acid induced resistance to Mungbean Yellow Mosaic India Virus in Vigna mungo. J Proteomics 74: 337-349.
16. Lin H, Tseng L (2010) DBCP: a web server for disulfide bonding connectivity pattern prediction without the prior knowledge of the bonding state of cysteines. Nucleic Acids Res 38(suppl 2): W503-W507.
17. Shen S, Yuxiang J, Kuang T (2003) Proteomics approach to identify wound-response related proteins from rice leaf sheath. Proteomics 3: 527-535.
18. Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M (2007) In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 1: 2856-2860.
19. Speicher KD, Kolbas O, Harper S, Speicher DW (2000) Systematic analysis of peptide recoveries from in-gel digestions for protein identifications in proteome studies. J Biomol Tech 11: 74-86.
20. Yin H, Yan F, Ji J, Li Y, Wang R, Xu C (2012) Proteomic analysis of Arabidopsis thaliana leaves infested by tobacco whitefly Bemisia tabaci (Gennadius) B biotype. Plant Mol Biol Rep 30: 379-390.
21. Zhou G, Yang LT, Li YR, Zou CL, Huang LP, Qiu LH, Huang X, Srivastava MK (2012) Proteomic analysis of osmotic stress-responsive proteins in sugarcane leaves. Plant Mol Biol Rep 30: 349-359.