M2 isoform of pyruvate kinase is dispensable for tumor maintenance and growth

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 2, 2013, Pages 489-494

  Cortés Cros, Marta ^a   Hemmerlin, Christelle ^a   Ferretti, Stephane ^a   Zhang, Juan ^a   Gounarides, John S ^b   Yin, Hong ^b   Muller, Alban ^a   Haberkorn, Anne ^a   Chene, Patrick ^a   Sellers, William R ^b   Hofmann, Francesco ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

^b NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOSE; GLUTAMINE; GLYCINE; ISOENZYME; LACTIC ACID; ONCOPROTEIN; PHOSPHOENOLPYRUVATE; PYRUVATE KINASE; PYRUVATE KINASE M2; SERINE; UNCLASSIFIED DRUG;

AMINO ACID SYNTHESIS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; FEMALE; GENE SILENCING; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; KNOCKOUT GENE; MOUSE; NONHUMAN; ONCOGENE; PRIORITY JOURNAL; PROTEIN FUNCTION; TUMOR CELL LINE; TUMOR GROWTH; TUMOR XENOGRAFT;

CARBON ISOTOPES; CELL LINE, TUMOR; CHROMATOGRAPHY, ION EXCHANGE; DNA PRIMERS; GENE KNOCKDOWN TECHNIQUES; GLYCOLYSIS; HUMANS; IMMUNOBLOTTING; LACTIC ACID; MAGNETIC RESONANCE SPECTROSCOPY; NEOPLASMS; PHOSPHOENOLPYRUVATE; PYRUVATE KINASE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TANDEM MASS SPECTROMETRY;

EID: 84872174405     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1212780110     Document Type: Article

References (19)

1. Warburg O (1956) On the origin of cancer cells. Science 123(3191):309-314.
2. Christofk HR, et al. (2008) The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature 452(7184):230-233.
3. Hitosugi T, et al. (2009) Tyrosine phosphorylation inhibits PKM2 to promote the Warburg effect and tumor growth. Sci Signal 2(97):ra73.
4. Cooper JA, Reiss NA, Schwartz RJ, Hunter T (1983) Three glycolytic enzymes are phosphorylated at tyrosine in cells transformed by Rous sarcoma virus. Nature 302 (5905):218-223.
5. Mazurek S, Boschek CB, Hugo F, Eigenbrodt E (2005) Pyruvate kinase type M2 and its role in tumor growth and spreading. Semin Cancer Biol 15(4):300- 308.
6. Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC (2008) Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature 452(7184):181-186.
7. Luo W, et al. (2011) Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxiainducible factor 1. Cell 145(5):732-744.
8. Lv L, et al. (2011) Acetylation targets the M2 isoform of pyruvate kinase for degradation through chaperone-mediated autophagy and promotes tumor growth. Mol Cell 42(6):719-730.
9. Macintyre AN, Rathmell JC (2011) PKM2 and the tricky balance of growth and energy in cancer. Mol Cell 42(6):713-714.
10. Mazurek S (2011) Pyruvate kinase type M2: A key regulator of the metabolic budget system in tumor cells. Int J Biochem Cell Biol 43(7):969-980.
11. DeBerardinis RJ, et al. (2007) Beyond aerobic glycolysis: Transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis. Proc Natl Acad Sci USA 104(49):19345-19350.
12. Dang CV (2009) PKM2 tyrosine phosphorylation and glutamine metabolism signal a different view of the Warburg effect. Sci Signal 2(97):pe75.
13. Yang C, et al. (2009) Glioblastoma cells require glutamate dehydrogenase to survive impairments of glucose metabolism or Akt signaling. Cancer Res 69(20):7986-7993.
14. Yang W, et al. (2011) Nuclear PKM2 regulates β-catenin transactivation upon EGFR activation. Nature 480(7375):118-122.
15. Vander Heiden MG, et al. (2010) Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science 329(5998):1492-1499.
16. Vander Heiden MG, et al. (2011) Metabolic pathway alterations that support cell proliferation. Cold Spring Harb Symp Quant Biol 76:325-334.
17. Jiang JK, et al. (2010) Evaluation of thieno[3,2-b]pyrrole[3,2-d] pyridazinones as activators of the tumor cell specific M2 isoform of pyruvate kinase. Bioorg Med Chem Lett 20(11):3387-3393.
18. Shimada N, Shinagawa T, Ishii S (2008) Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein. Genes Cells 13(3):245-254.
19. Sekiguchi Y, Mitsuhashi N, Kokaji T, Miyakoda H, Mimura T (2005) Development of a comprehensive analytical method for phosphate metabolites in plants by ion chromatography coupled with tandem mass spectrometry. J Chromatogr A 1085(1):131-136.