Three-dimensional selection of leptin aptamers using capillary electrophoresis and implications for clone validation

Analytical Biochemistry

Volumn 434, Issue 1, 2013, Pages 146-152

  Ashley, Jon ^a   Li, Sam F Y ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Aptamer; Binding constant determination; CE SELEX; Leptin; Nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)

Indexed keywords

BINDING ENERGY; DNA; FLUORESCENCE; HORMONES; MIXTURES; PROTEINS; SYSTEMATIC ERRORS;

APTAMERS; BINDING AFFINITIES; BINDING CONSTANT; FLUORESCENCE INTENSITIES; LEPTIN; NON-EQUILIBRIUM CAPILLARY ELECTROPHORESIS OF EQUILIBRIUM MIXTURES; SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENTS; THREE-DIMENSIONAL ENVIRONMENT;

CAPILLARY ELECTROPHORESIS;

APTAMER; LEPTIN;

ARTICLE; BINDING AFFINITY; CAPILLARY ELECTROPHORESIS; CAPILLARY ELECTROPHORESIS SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT; CONTROLLED STUDY; FLUORESCENCE; PRIORITY JOURNAL; PROTEIN BINDING; VALIDATION PROCESS;

EID: 84872138565     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.11.024     Document Type: Article

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