Standard Addition Quantitative Real-Time PCR (SAQPCR): A Novel Approach for Determination of Transgene Copy Number Avoiding PCR Efficiency Estimation

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Huang, Yuji ^a   Yin, Xueren ^a   Zhu, Changqing ^a   Wang, Weiwei ^a   Grierson, Donald ^a,b   Xu, Changjie ^a   Chen, Kunsong ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA;

ARTICLE; GENE DOSAGE; GENETIC ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT TISSUE; REAL TIME POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; STANDARD ADDITION QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION; TOMATO; TRANSGENE; ZYGOSITY;

BLOTTING, SOUTHERN; DNA; GENE DOSAGE; LYCOPERSICON ESCULENTUM; PLANTS, GENETICALLY MODIFIED; PLASMIDS; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; TRANSGENES;

LYCOPERSICON ESCULENTUM;

EID: 84872133599     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0053489     Document Type: Article

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