Validation of a HPLC method for the measurement of erythrocyte encapsulated thymidine phosphorylase (EE-TP) activity

Journal of Pharmaceutical and Biomedical Analysis

Volumn 76, Issue , 2013, Pages 8-12

  Fairbanks, Lynette D ^a   Levene, Michelle ^b   Bax, Bridget E ^b  

^a Purine Research Laboratory   (United Kingdom)

^b ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Erythrocyte encapsulated thymidine phosphorylase; HPLC; MNGIE; Thymidine phosphorylase; Validation

Indexed keywords

AMMONIUM ACETATE; ERYTHROCYTE ENCAPSULATED THYMIDINE PHOSPHORYLASE; ETHER; SODIUM DIHYDROGEN PHOSPHATE; TETRABUTYLAMMONIUM; THYMIDINE PHOSPHORYLASE; TRICHLOROACETIC ACID; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CELL LYSATE; CENTRIFUGATION; DRUG DETERMINATION; ENZYME ACTIVITY; FLOW RATE; INCUBATION TIME; MNGIE SYNDROME; PH; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY ANALYSIS; SUPERNATANT; ULTRAVIOLET RADIATION;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; ERYTHROCYTES; HUMANS; LIMIT OF DETECTION; SENSITIVITY AND SPECIFICITY; THYMIDINE; THYMIDINE PHOSPHORYLASE;

EID: 84872044393     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2012.12.006     Document Type: Article

References (13)

1. Hirano M., Silvestri G., Blake D.M., Lombes A., Minetti C., Bonilla E., Hays A.P., Lovelace R.E., Butler I., Bertorini T.E., Threlkeld A.B., Mitsumoto H., Salberg L.M., Rowland L.P., DiMauro S. Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE): clinical, biochemical, and genetic features of an autosomal recessive mitochondrial disorder. Neurology 1994, 44:721-727.
2. Marti R., Nishigaki Y., Hirano M. Elevated plasma deoxyuridine in patients with thymidine phosphorylase deficiency. Biochem. Biophys. Res. Commun. 2003, 303:14-18.
3. Moran N.F., Bain M.D., Muqit M., Bax B.E. Carrier erythrocyte entrapped thymidine phosphorylase therapy in MNGIE. Neurology 2008, 71:686-688.
4. Godfrin Y., Horand F., Franco R., Dufour E., Kosenko E., Bax B.E., Banz A., Skorokhod O.A., Lanao J.M., Vitvitsky V., Sinauridze E., Bourgeaux V., Gunter K.C. Meeting highlights: international seminar on the red blood cells as vehicles for drugs. Expert. Opin. Biol. Ther. 2012, 12:127-133.
5. Godfrin Y., Bax B.E. Enzyme bioreactors as drugs. Drugs Future 2012, 37:263-272.
6. Levene M., Bain M.D., Fairbanks L.D., Moran N.F., Bax B.E. Erythrocyte encapsulated thymidine phosphosphorylase (EE-TP) for the treatment of mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). J. Inherit. Metab. Dis. 2012, 35:S150.
7. Gan T.E., Hallam L., Pilkington G.R., van der Weyden M.B. A rapid and simple radiometric assay for thymidine phosphorylase of human peripheral blood cells. Clin. Chim. Acta 1981, 116:231-236.
8. Marti R., Spinazzola A., Tadesse S., Nishino I., Nishigaki Y., Hirano M. Definitive diagnosis of mitochondrial neurogastrointestinal encephalomyopathy by biochemical assays. Clin. Chem. 2004, 50:120-124.
9. van Kuilenbur A.B.P., Zoetekouw L. Determination of thymidine phosphorylase activity by a non-radiochemical assay using reversed-phase high-performance liquid chromatography. J. Chromatogr. B 2005, 820:271-275.
10. International Conference on Harmonisation (ICH) (2005, Nov) Harmonised Tripartite Guideline Q2 (R1) Validation of Analytical Procedures: Text and Methodology.
11. Bax B.E., Bain M.D., Talbot P.J., Parker-Williams E.J., Chalmers R.A. In vivo survival of human carrier erythrocytes. Clin. Sci. 1999, 96:171-178.
12. Bax B.E., Bain M.D., Fairbanks L.D., Webster A.D.B., Chalmers R.A. In vitro and in vivo studies of human carrier erythrocytes loaded with polyethylene glycol-conjugated and native adenosine deaminase. Br. J. Haematol. 2000, 109:549-554.
13. Bax B.E., Bain M.D., Fairbanks L.D., Webster A.D.B., Ind P.W., Hershfield M.S., Chalmers R.A. A nine year evaluation of carrier erythrocyte encapsulated adenosine deaminase therapy in a patient with adult-type adenosine deaminase deficiency. Eur. J. Haematol. 2007, 79:338-348.