Dissecting eukaryotic cells by coherent phase microscopy: Quantitative analysis of quiescent and activated T lymphocytes

Journal of Biomedical Optics

Volumn 17, Issue 7, 2012, Pages

  Tychinsky, Vladimir P ^a   Kretushev, Alexander V ^a   Vyshenskaya, Tatiana V ^b   Shtil, Alexander A ^c  

^a MIREA RUSSIAN TECHNOLOGICAL UNIVERSITY   (Russian Federation)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

^c N N BLOKHIN RUSSIAN CANCER RESEARCH CENTER   (Russian Federation)

Author keywords

Coherent phase microscopy; Optical model of living cell; Phytohemagglutinin; Subcellular structures; T lymphocyte

Indexed keywords

ARTICLE; AUDIOVISUAL EQUIPMENT; AUTOMATED PATTERN RECOGNITION; BIOLOGICAL MODEL; CELL CULTURE; COMPUTER ASSISTED DIAGNOSIS; COMPUTER SIMULATION; CYTOLOGY; DISSECTION; HUMAN; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METHODOLOGY; PHASE CONTRAST MICROSCOPY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; T LYMPHOCYTE;

CELLS, CULTURED; COMPUTER SIMULATION; DISSECTION; HUMANS; IMAGE INTERPRETATION, COMPUTER-ASSISTED; LYMPHOCYTE ACTIVATION; MICROSCOPY, PHASE-CONTRAST; MODELS, ANATOMIC; MODELS, IMMUNOLOGICAL; PATTERN RECOGNITION, AUTOMATED; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; T-LYMPHOCYTES;

EID: 84872043031     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.JBO.17.7.076020     Document Type: Article

References (18)

1. Y. Park et al., "Metabolic remodeling of the human red blood cell membrane," Proc. Natl. Acad. Sci. U. S. A. 107(4), 1289-1294 (2010).
2. Y. Park et al., "Static and dynamic light scattering of healthy malaria-parasite invaded red blood cells," J. Biomed. Opt. 15(2), 020506 (2010).
3. Y Park et al., "Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum," Proc. Natl. Acad. Sci. U. S. A. 105(37), 13730-13753 (2008).
4. B. Rappaz et al., "Erythrocyte volume and refractive index measurement with digital holographic microscope," Proc. SPIE 6445, 644509, (2007).
5. B. Rappaz et al., "Measurements of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy," Opt. Express 13(23), 9361-9373 (2005). (Pubitemid 41611969)
6. G. Popescu, "Quantitative phase imaging of nanoscale cell structure and dynamics," in Methods in Cell Biology, B. Jena, Ed., Vol. 90, pp. 87-115, Elsevier Inc., Amsterdam, (2008).
7. V. Tychinsky, "The metabolic component of cellular refractivity and its importance for optical cytometry," J. Biophotonics 2(8-9), 494-504 (2009).
8. Z. Wang et al., "Tissue refractive index as marker of disease," J. Biomed. Opt. 16(11), 116017 (2011).
9. V. P. Tychinsky and A. N. Tikhonov, "Interference microscopy in cell biophysics. Principles and methodological aspects of coherent phase microscopy," Cell Biochem. Biophys. 58(3), 107-116 (2010).
10. V. P. Tychinsky and A. N. Tikhonov, "Visualization of individual cells and energy-transducing organelles," Cell Biochem. Biophys. 58(3), 117-128 (2010).
11. Z. Wang et al., "Spatial light interference microscopy (SLIM)," Opt. Express 19(2), 1016-1026 (2011).
12. V P. Tychinsky, "Dynamic phase microscopy: is a "dialog" with the cell possible," Physics-Uspekhi 50(5), 513-528 (2007).
13. V P. Tychinsky, "Super-resolution and singularities in phase images," Phys. Uspekhi 51(11), 1161-1169 (2008).
14. Y Hiramoto, S. Shumei, and S. Yoko, "Refractive index of the protoplasm in sea urchin eggs," Develop. Growth Differ. 21(2), 141-153 (1979). (Pubitemid 10247131)
15. V Manteifel, L. Bakeeva, and T Karu, "Ultrastructural changes in chondriome of human lymphocytes after irradiation with He-Ne laser: appearance of giant mitochondria," J. Photochem. Photobiol. 38(1), 25-30 (1997). (Pubitemid 27183292)
16. V M. Manteifel and T I. Karu, "Loosening of condensed chromatin in human blood lymphocytes exposed to irradiation with a low-energy He-Ne laser," Izv. Akad. Nauk Ser. Biol. 36(6), 654-661 (2009).
17. V Manteifel and T Karu, "Ultrastructural changes in chondriome of human lymphocytes after irradiation with He-Ne laser: appearance of giant mitochondria," J. Photochem. Photobiol. 38, 25-30 (1997). (Pubitemid 27183292)
18. M. Mir, K. Tangella, and G. Popescu, "Blood testing at the single cell level using quantitative phase and amplitude microscopy," Biomed. Opt. Express 2(12), 3259-3266 (2011).