Development and validation of LC-MS/MS method for the estimation of β-hydroxy-β-methylbutyrate in rat plasma and its application to pharmacokinetic studies

Biomedical Chromatography

Volumn 27, Issue 2, 2013, Pages 142-147

  Deshpande, Parag ^a   Jie, Zhang ^b   Subbarayan, Ramesh ^a   Mamidi, Vijay Kumar ^a   Chunduri, Raja Haranadha Babu ^a   Das, Tapas ^b   Shreeram, Sathyavageeswaran ^b  

^a SYNGENE INTERNATIONAL LTD   (India)

^b ABBOTT LABORATORIES   (United States)

Author keywords

Bioavailability; LC MS MS; Pharmacokinetics; Rat plasma; hydroxy methylbutyrate

Indexed keywords

BIOCHEMISTRY; BODY FLUIDS; ELECTROSPRAY IONIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PLASMA STABILITY; RATS;

BIOAVAILABILITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRIES; INTERNAL STANDARDS; ITS APPLICATIONS; LC-MS-MS; LC.MS/MS; PHARMACOKINETIC STUDIES; RAT PLASMA; SIMPLE++; Β-HYDROXY-Β-METHYLBUTYRATE;

PHARMACOKINETICS;

BETA HYDROXY BETA METHYLBUTYRATE; BUTYRIC ACID DERIVATIVE; UNCLASSIFIED DRUG; WARFARIN;

ACCURACY; ANIMAL EXPERIMENT; ARTICLE; CONCENTRATION (PARAMETERS); DRUG SELECTIVITY; DRUG STABILITY; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; LIQUID LIQUID EXTRACTION; MASS SPECTROMETRY; MAXIMUM PLASMA CONCENTRATION; NONHUMAN; RAT; TIME TO MAXIMUM PLASMA CONCENTRATION;

RATTUS;

EID: 84871968007     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.2758     Document Type: Article

References (10)

1. Aversa Z, Bonetto A, Costelli P, Minero VG, Penna F, Baccino FM, Lucia S, Rossi FF and Muscaritoli M. β-hydroxy-β-methylbutyrate (HMB) attenuates muscle and body weight loss in experimental cancer cachexia. International Journal of Oncology 2011; 38: 713-720.
2. Baracos VE. Management of muscle wasting in cancer-associated cachexia: understanding gained from experimental studies. Cancer 2001; 92: 1669-1677.
3. Eley HL, Russell ST, Baxter JH, Mukerji P and Tisdale MJ. Signaling pathways initiated by β-hydroxy-β-methylbutyrate to attenuate the depression of protein synthesis in skeletal muscle in response to cachectic stimuli. American Journal of Physiology - Endocrinology and Metabolism 2007; 293: E923-E931.
4. Kornasio R, Riederer I, Butler-Browne G, Mouly V, Uni Z and Halevy O. β-Hydroxy-β-methylbutyrate (HMB) stimulates myogenic cell proliferation, differentiation and survival via the MAPK/ERK and PI3K/Akt pathways. Biochimica et Biophysica Acta 2009; 1793: 755-763.
5. Nissen S, Van Koevering M and Webb D. Analysis of β-Hydroxy-β-methylbutyrate in plasma by gas chromatography and mass spectrometry. Analytical Biochemistry 1990; 188: 17-19.
6. Nissen S, Sharp R, Ray M, Rathmacher JA, Rice D, Fuller JC Jr, Connelly AS and Abumrad N. Effect of leucine metabolite β-hydroxy-β-methylbutyrate on muscle metabolism during resistance-exercise training. Journal of Applied Physiology 1996; 81: 2095-2104.
7. Slater GJ and Jenkins D. β-Hydroxy-β-methylbutyrate (HMB) supplementation and the promotion of muscle growth and strength. Sports Medicine 2000; 30: 105-116.
8. US Food and Drug Administration. FDA Guidance for Industry: Bioanalytical Method Validation. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research: Rockville, MD, 2001.
9. Vukovich MD, Slater G, Macchi MB, Turner MJ, Fallon K, Boston T and Rathmacher J. β-Hydroxy-β-methylbutyrate (HMB) kinetics and the influence of glucose ingestion in humans. Journal of Nutritional Biochemistry 2001; 12: 631-639.
10. Zhang Z, Rathmacher J, Coates C and Nissen S. Occurrence of β-hydroxy-β-methylbutyrate in foods and feeds. FASEB Journal 1994; 8: A464 (Abstract 2685).