Analysis of photoreceptor outer segment phagocytosis by RPE cells in culture

Methods in Molecular Biology

Volumn 935, Issue , 2013, Pages 285-295

  Mao, Yingyu ^a   Finnemann, Silvia C ^a  

^a FORDHAM UNIVERSITY   (United States)

Author keywords

Cell culture; Cell receptors; Engulfment; Fluorescence scanning; Immunoblotting; Immunofluorescence microscopy; Phagocytosis; Photoreceptor outer segments; Quantification; Recognition; Retinal pigment epithelium

Indexed keywords

OPSIN;

ANIMAL; ARTICLE; CELL CULTURE; CELL SEPARATION; CULTURE TECHNIQUE; CYTOLOGY; FLUORESCENCE MICROSCOPY; IMMUNOBLOTTING; METHODOLOGY; PHAGOCYTOSIS; PHOTORECEPTOR OUTER SEGMENT; PIGMENT EPITHELIUM; SWINE; ULTRASTRUCTURE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; IMMUNOBLOTTING; MICROSCOPY, FLUORESCENCE; OPSINS; PHAGOCYTOSIS; RETINAL PHOTORECEPTOR CELL OUTER SEGMENT; RETINAL PIGMENT EPITHELIUM; SWINE;

SUS;

EID: 84871951857     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-080-9-20     Document Type: Article

References (11)

1. Strauss O (2005) The retinal pigment epithelium in visual function. Physiol Rev 85:845-881 (Pubitemid 40885218)
2. LaVail MM (1976) Rod outer segment disk shedding in rat retina: Relationship to cyclic lighting. Science 194:1071-1074
3. Nandrot EF, Kim Y, Brodie SE, Huang X, Sheppard D, Finnemann SC (2004) Loss of synchronized retinal phagocytosis and agerelated blindness in mice lacking a v b 5 integrin. J Exp Med 200:1539-1545 (Pubitemid 40094178)
4. Mayerson PL, Hall MO (1986) Rat retinal pigment epithelial cells show speci fi city of phagocytosis in vitro. J Cell Biol 103:299-308 (Pubitemid 16067013)
5. Nabi IR, Mathews AP, Cohen-Gould L, Gundersen D, Rodriguez-Boulan E (1993) Immortalization of polarized rat retinal pigment epithelium. J Cell Sci 104:37-49 (Pubitemid 23073166)
6. Davis AA, Bernstein PS, Bok D, Turner J, Nachtigal M, Hunt RC (1995) A human retinal pigment epithelial cell line that retains epithelial characteristics after prolonged culture. Invest Ophthalmol Vis Sci 36:955-964
7. Dunn KC, Aotaki-Keen AE, Putkey FR, Hjelmeland LM (1996) ARPE-19, a human retinal pigment epithelial cell line with differentiated properties. Exp Eye Res 62:155-169 (Pubitemid 26140102)
8. Finnemann SC, Bonilha VL, Marmorstein AD, Rodriguez-Boulan E (1997) Phagocytosis of rod outer segments by retinal pigment epithelial cells requires a v b 5 integrin for binding but not for internalization. Proc Natl Acad Sci U S A 94:12932-12937 (Pubitemid 27518454)
9. Molday RS, Hicks D, Molday L (1987) Peripherin. A rim-speci fi c membrane protein of rod outer segment discs. Invest Ophthalmol Vis Sci 28:50-61 (Pubitemid 17230190)
10. Finnemann SC (2003) Focal adhesion kinase signaling promotes phagocytosis of integrinbound photoreceptors. EMBO J 22: 4143-4154 (Pubitemid 37021743)
11. Adamus G, Zam ZS, Arendt A, Palczewski K, McDowell JH, Hargrave PA (1991) Antirhodopsin monoclonal antibodies of de fi ned speci fi city: Characterization and application. Vision Res 31:17-31