Isolation and culture of mesenchymal stem cells from mouse compact bone

Methods in Molecular Biology

Volumn 946, Issue , 2013, Pages 335-347

  Short, Brenton ^a   Wagey, Ravenska ^a  

^a STEMCELL Technologies   (Canada)

Author keywords

CFU F; Colony forming unit fibroblast; Compact bone; Mesenchymal Stem Cell; MSC; Multipotent

Indexed keywords

ANIMALIA; RODENTIA;

EID: 84871897089     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-128-8_21     Document Type: Article

References (7)

1. Simmons PJ, Torok-Storb B (1991) Identification of stromal cell precursors inhuman bone marrow by a novel monoclonal antibody, STRO-1. Blood 78(1):55-62
2. Gronthos S et al (2003) Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci 116(Pt 9):1827-1835
3. Phinney DG et al (1999) Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation. J Cell Biochem 72(4): 570-585
4. Friedenstein AJ, Gorskaja JF, Kulagina NN (1976) Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 4(5):267-274
5. Taichman RS, Wang Z, Shiozawa Y, Jung Y, Song J, Balduino A, Wang J, Patel LR, Havens AM, Kucia M, Ratajczak MZ, Krebsbach PH (2010) Prospective identification and skeletal localization of cells capable of multilineage differentiation in vivo. Stem Cells Dev 19(10): 1557-1570
6. Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, Suzuki S, Miyauchi-Hara C, Nagoshi N, Sunabori T, Shimmura S, Miyawaki A, Nagagawa T, Suda T, Okano H, Matsuzaki Y (2009) Prospective identification, isolation, and systemic transplantation of multipotent mesen-chymal stem cells in murine bone marrow. J Exp Med 206(11):2483-2496
7. Zhu H, Guo ZK, Jiang XX, Li H, Wang XY, Yao HY, Zhang Y, Mao N (2010) A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. Nat Protoc 5:550-560