Standardization of the filovirus plaque assay for use in preclinical studies

Viruses

Volumn 4, Issue 12, 2012, Pages 3511-3530

  Shurtleff, Amy C ^a   Biggins, Julia E ^a   Keeney, Ashley E ^a   Zumbrun, Elizabeth E ^a   Bloomfield, Holly A ^a   Kuehne, Ana ^a   Audet, Jennifer L ^a   Alfson, Kendra J ^b   Griffiths, Anthony ^b   Olinger, Gene G ^a   Bavari, Sina ^a  

^a UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

^b SOUTHWEST FOUNDATION FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Ebola; Ebolavirus; Filovirus; Marburg virus; Marburgvirus; Plaque assay; Vero

Indexed keywords

ANIMAL CELL; ARTICLE; BIOASSAY; CELL TYPE; CONTROLLED STUDY; EBOLA VIRUS; FILOVIRUS; FILOVIRUS PLAQUE ASSAY; GENETIC VARIABILITY; INCUBATION TIME; INOCULATION; MARBURG VIRUS; NONHUMAN; PRACTICE GUIDELINE; STANDARDIZATION; VALIDATION STUDY; VERO CELL; VIRUS PLAQUE;

ANIMALS; CERCOPITHECUS AETHIOPS; EBOLAVIRUS; MARBURGVIRUS; VERO CELLS; VIRAL LOAD; VIRAL PLAQUE ASSAY;

ANIMALIA; EBOLA VIRUS; LAKE VICTORIA MARBURGVIRUS; MARBURGVIRUS;

EID: 84871887989     PISSN: 19994915     EISSN: None     Source Type: Journal    
DOI: 10.3390/v4123511     Document Type: Article

References (22)

1. Kuhn, J.H.; Dodd, L.E.; Wahl-Jensen, V.; Radoshitzky, S.R.; Bavari, S.; Jahrling, P.B. Evaluation of perceived threat differences posed by filovirus variants. Biosecur. Bioterror. 2011, 9, 361-371.
2. Guidance for Industry-Antiviral Product Development-Conducting and Submitting Virology Studies to the Agency. In US Depart of Health and Human Services, F.D.A., Center for Drug Evaluation, Ed.; Fed Regist: 2006; Volume 70, pp. 30127-30128.
3. Warren, T.K.; Warfield, K.L.; Wells, J.; Swenson, D.L.; Donner, K.S.; van Tongeren, S.A.; Garza, N.L.; Dong, L.; Mourich, D.V.; Crumley, S.; et al. Advanced antisense therapies for postexposure protection against lethal filovirus infections. Nat. Med. 2010, 16, 991-994.
4. Warfield, K.L.; Swenson, D.L.; Olinger, G.G.; Nichols, D.K.; Pratt, W.D.; Blouch, R.; Stein, D.A.; Aman, M.J.; Iversen, P.L.; Bavari, S. Gene-specific countermeasures against Ebola virus based on antisense phosphorodiamidate morpholino oligomers. PLoS Pathog. 2006, 2, e1.
5. Geisbert, T.W.; Lee, A.C.; Robbins, M.; Geisbert, J.B.; Honko, A.N.; Sood, V.; Johnson, J.C.; de Jong, S.; Tavakoli, I.; Judge, A.; et al. Postexposure protection of non-human primates against a lethal Ebola virus challenge with RNA interference: A proof-of-concept study. Lancet 2010, 375, 1896-1905.
6. Cooper, P.D. The plaque assay of animal viruses. Adv. Virus Res. 1961, 8, 319-378.
7. Earley, E.; Johnson, K. The Lineage of Vero, Vero 76 and Its Clone C1008 in the United States; Department of Microbiology, School of Medicine, Chiba University: Tokyo, Japan, 1988.
8. Moe, J.B.; Lambert, R.D.; Lupton, H.W. Plaque assay for Ebola virus. J. Clin. Microbiol. 1981, 13, 791-793.
9. Kiley, M.P.; Regnery, R.L.; Johnson, K.M. Ebola virus: Identification of virion structural proteins. J. Gen. Virol. 1980, 49, 333-341.
10. Bushar, G.; Sagripanti, J.L. Method for improving accuracy of virus titration: Standardization of plaque assay for Junin virus. J. Virol. Methods 1990, 30, 99-107.
11. Payne, A.F.; Binduga-Gajewska, I.; Kauffman, E.B.; Kramer, L.D. Quantitation of flaviviruses by fluorescent focus assay. J. Virol. Methods 2006, 134, 183-189.
12. Mitchell, S.W.; McCormick, J.B. Physicochemical inactivation of Lassa, Ebola, and Marburg viruses and effect on clinical laboratory analyses. J. Clin. Microbiol. 1984, 20, 486-489.
13. Bray, M.; Hatfill, S.; Hensley, L.; Huggins, J.W. Haematological, biochemical and coagulation changes in mice, guinea-pigs and monkeys infected with a mouse-adapted variant of Ebola Zaire virus. J. Comp. Pathol. 2001, 125, 243-253.
14. Geisbert, T.W.; Daddario-Dicaprio, K.M.; Geisbert, J.B.; Reed, D.S.; Feldmann, F.; Grolla, A.; Stroher, U.; Fritz, E.A.; Hensley, L.E.; Jones, S.M.; et al. Vesicular stomatitis virus-based vaccines protect nonhuman primates against aerosol challenge with Ebola and Marburg viruses. Vaccine 2008, 26, 6894-6900.
15. Scott, E.P.; Aronson, J.F. Cytokine patterns in a comparative model of arenavirus haemorrhagic fever in guinea pigs. J. Gen. Virol. 2008, 89, 2569-2579.
16. Geisbert, T.W.; Jahrling, P.B. Use of immunoelectron microscopy to show Ebola virus during the 1989 United States epizootic. J. Clin. Pathol. 1990, 43, 813-816.
17. GraphPad Prism software, version 5; GraphPad Software, Inc: La Jolla, CA, USA, 2007
18. Wright, S. The theoretical variance within and among subdivisions of a population that is in a steady state. Genetics 1952, 37, 312-321.
19. Titenko, A.M.; Novozhilov, S.S.; Andaev, E.I.; Borisova, T.I.; Kulikova, E.V. Ebola virus reproduction in cell cultures. Vopr. Virusol. 1992, 37, 110-113.
20. Schafer, S.L.; Lin, R.; Moore, P.A.; Hiscott, J.; Pitha, P.M. Regulation of type I interferon gene expression by interferon regulatory factor-3. J. Biol. Chem. 1998, 273, 2714-2720.
21. Matrosovich, M.; Matrosovich, T.; Garten, W.; Klenk, H.D. New low-viscosity overlay medium for viral plaque assays. Virol. J. 2006, 3, 63.
22. USAMRIID, unpublished data. Amy Shurtleff, Ana Keuhne, Julia Biggins and Ashley Keeney. USAMRIID Fort Detrick, MD, USA. Use of alternative overlays in plaque assays. Unpublished work, 2011.