Isolation, enumeration, and expansion of human mesenchymal stem cells in culture

Methods in Molecular Biology

Volumn 946, Issue , 2013, Pages 315-334

  Wagey, Ravenska ^a   Short, Brenton ^a  

^a STEMCELL Technologies   (Canada)

Author keywords

Bone marrow mononuclear cells; CFU F; Expansion; FBS containing medium; Human mesenchymal stem cells; Xeno free medium

Indexed keywords

BOVINAE;

EID: 84871875975     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-128-8_20     Document Type: Article

References (28)

1. Bruder SP, Jaiswal N, Haynesworth SE (1997) Growth kinetics, self-renewal, and osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem 64:278-294
2. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF (1998) Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng 4:415-428
3. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesen-chymal stem cells. Science 284:143147
4. Erices A, Conget P, Minguell JJ (2000) Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 109:235-242
5. Debari C, Dell'Accio F, Tylazanowski P et al (2001) Multipotent mesenchymal stem cells form adult human synovial membrane. Arthritis Rheum 44:1928-1942
6. Kuznetsov SA, Mankani MH, Gronthos S et al (2001) Circulating skeletal stem cells. J Cell Biol 153:113-114
7. Tondreau T, Meuleman N, Delforge A et al (2005) Mesenchymal stem cells derived from CD133 positive cells in mobilized peripheral blood and cord blood: proliferation, Oct4 expression, and plasticity. Stem Cells 23:1105-1112
8. Roberts IA, Campagnoli IA, Kumar S et al (2001) Identification of mesenchymal stem/progenitor cells in humanfirst trimester fetal blood, liver and bone marrow. Blood 98:2396-2402
9. Int'l Anker PS, Scherjon SA, Kleiburg-van der Keur C et al (2003) Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation. Blood 102:1548-1549
10. Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279-4295
11. Crisan M, Chen CW, Corselli M, Andriolo G, Lazzari L, Péault B (2009) Perivascular multipotent progenitor cells in human organs. Ann N Y Acad Sci 1176:118-123
12. Friedenstein AJ, Chailakhjan RK, Lalykina KS (1970) The development offibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 3:393-403
13. Friedenstein AJ (1980) Stromal mechanisms of bone marrow: cloning in vitro and transplantation in vivo. Haematol Blood Transfus 25:19-29
14. Clarke E, McCann SR (1989) Age dependent in vitro stromal growth. Bone Marrow Transplant 4:596-597
15. Gronthos S, Graves SE, Ohta S, Simmons PJ (1994) The STRO-1+ fraction of adult human bone marrow contains the osteogenic precursors. Blood 84:4164-4173
16. Sekiya I, Vuoristo JT, Larson BL, Prockop DJ (2002) In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. Proc Natl Acad Sci U S A 99:4397-4402
17. Russell KC, Phinney DG, Lacey MR, Barrilleaux BL, Meyertholen KE, O'Connor KC (2010) In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment. Stem Cells 28:788-798
18. Phinney DG, Prockop DJ (2007) Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair-current views. Stem Cells 25:2896-2902
19. Deng J, Petersen BE, Steindler DA, Jorgensen ML, Laywell ED (2006) Mesenchymal stem cells spontaneously express neural proteins in culture and are neurogenic after transplantation. Stem Cells 24:1054-1064
20. Tsuji H, Miyoshi S, Ikegami Y, Hida N, Asada H, Togashi I, Suzuki J, Satake M, Nakamizo H, Tanaka M, Mori T, Segawa K, Nishiyama N, Inoue J, Makino H, Miyado K, Ogawa S, Yoshimura Y, Umezawa A (2010) Xenografted human amniotic membrane-derived mesen-chymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytes. Circ Res 106:1613-1623
21. Horwitz EM, Prockop DJ, Fitzpatrick LA, Koo WWK, Gordon PL, Neel M, Sussman M, Orchard P, Marx JC, Pyeritz RE, Brenner MK (1999) Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med 5:309-313
22. Lazarus HM, Koc ON, Devine SM, Curtin P, Maziarz RT, Holland HK, Shpall EJ, McCarthy P, Atkinson K, Cooper BW, Gerson SL, Laughlin MJ, Loberiza FR Jr, Moseley AB, Bacigalupo A (2005) Cotransplantation of HLA-identical sibling culture-expanded mes-enchymal stem cells and hematopoietic stem cells in hematologic malignancy patients. Biol Blood Marrow Transplant 11:389-398
23. Le Blanc K, Frassoni F, Ball L, Locatelli F, Roelofs H, Lewis I, Lanino E, Sundberg B, Bernardo ME, Remberger M, Dini G, Egeler RM, Bacigalupo A, Fibbe W, Ringdén O, Group for Blood and Marrow Transplantation (2008) Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study. Lancet 371:1579-86
24. Meuleman N, Tondreau T, Delforge A et al (2006) Human marrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical alpha-MEM medium. Eur J Haematol 76:309-316
25. Kocaoemer A, Kern S, Kluter H, Bieback K (2007) Human AB-serum and thrombin-activated platelet-rich plasma are suitable alterna tives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 25:1270-1278
26. Schallmoser K, Bartmann C, Rohde E et al (2007) Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion 47:1436-1446
27. Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM (2001) Purification and ex vivo expansion of postnatal human marrow meso-dermal progenitor cells. Blood 98:2615-2625
28. D'Ippolito G, Diabira S, Howard GA, Menei P, Roos BA, Schiller PC (2004) Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential. J Cell Sci 117:2971-2981