Identification of novel mutant p53 interacting proteins by proteomic analysis

Methods in Molecular Biology

Volumn 962, Issue , 2013, Pages 85-94

  Deb, Sumitra ^a   Graves, Paul R ^b  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b NEW YORK METHODIST HOSPITAL   (United States)

Author keywords

Co precipitation; Mass spectrometry; Mutant p53; Proteomics

Indexed keywords

EID: 84871869428     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-236-0_7     Document Type: Article

References (7)

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2. Kerppola TK (2008) Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. Annu Rev Biophys 37:465-487
3. Sankala H, Vaughan C, Wang J, Deb S, Graves PR (2011) Upregulation of the mitochondrial transport protein, Tim50, by mutant p53 contributes to cell growth and chemoresistance. Arch Biochem Biophys 512:52-60
4. Deb D, Scian M, Roth KE, Li W, Keiger J, Chakraborti AS, Deb SP, Deb S (2002) Hetero-oligomerization does not compromise 'gain of function' of tumor-derived p53 mutants. Oncogene 21:176-189
5. Scian MJ, Stagliano KE, Deb D, Ellis MA, Carchman EH, Das A, Valerie K, Deb SP, Deb S (2004) Tumor-derived p53 mutants induce oncogenesis by transactivating growth-promoting genes. Oncogene 23:4430-4443
6. Lanyi A, Deb D, Seymour RC, Ludes-Meyers JH, Subler MA, Deb S (1998) 'Gain of function' phenotype of tumor-derived mutant p53 requires the oligomerization/nonsequence-specific nucleic acid-binding domain. Oncogene 16:3169-3176
7. Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68:850-858