Development and application of an indirect ELISA for detection of antibodies against avian hepatitis E virus

Journal of Virological Methods

Volumn 187, Issue 1, 2013, Pages 32-36

  Zhao, Qin ^a   Sun, Yani ^a   Zhao, Jinan ^a   Hu, Shoubin ^b   Zhao, Feifei ^b   Chen, Fuyong ^c   Clavijo, Alfonso ^d   Zhou, En Min ^a   Xiao, Yihong ^b  

^a NORTHWEST A F UNIVERSITY   (China)

^b SHANDONG AGRICULTURAL UNIVERSITY   (China)

^c CHINA AGRICULTURAL UNIVERSITY   (China)

^d TEXAS A AND M UNIVERSITY SYSTEM   (United States)

Author keywords

Avian hepatitis E virus; Indirect ELISA; ORF2 protein; Serology

Indexed keywords

AMINO ACID; IMMUNOGLOBULIN G ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIBODY DETECTION; ANTIBODY TITER; ARTICLE; BLOOD SAMPLING; CARBOXY TERMINAL SEQUENCE; CHICKEN; CHINA; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DILUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; HEPATITIS E; HEPATITIS E VIRUS; INDIRECT ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; RECEIVER OPERATING CHARACTERISTIC; REFERENCE VALUE; SENSITIVITY AND SPECIFICITY; SEROPREVALENCE; VIRUS ISOLATION; VIRUS STRAIN; WESTERN BLOTTING;

ANIMALS; CHICKENS; CHINA; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEPATITIS ANTIBODIES; HEPATITIS, VIRAL, ANIMAL; HEPEVIRUS; IMMUNOGLOBULIN G; POULTRY DISEASES; RNA VIRUS INFECTIONS; SEROEPIDEMIOLOGIC STUDIES;

AVES; AVIAN HEPATITIS E VIRUS;

EID: 84871793464     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.08.026     Document Type: Article

References (15)

1. Agunos A.C., Yoo D., Youssef S.A., Ran D., Binnington B., Hunter D.B. Avian hepatitis E virus in an outbreak of hepatitis-splenomegaly syndrome and fatty liver haemorrhage syndrome in two flaxseed-fed layer flocks in Ontario. Avian Pathology 2006, 35:404-412.
2. Bilic I., Jaskulska B., Basic A., Morrow C.J., Hess M. Sequence analysis and comparison of avian hepatitis E viruses from Australia and Europe indicate the existence of different genotypes. Journal of General Virology 2009, 90:863-873.
3. Billam P., Huang F.F., Sun Z.F., Pierson F.W., Duncan R.B., Elvinger F., Guenette D.K., Toth T.E., Meng X.J. Systematic pathogenesis and replication of avian hepatitis E virus specific-pathogen free adult chickens. Journal of Virology 2005, 79:3429-3437.
4. Dong S.W., Zhao Q., Lu M.Z., Sun P., Qiu H.K., Zhang L., Lv J.H., Zhou E.M. Analysis of epitopes in the capsid protein of avian hepatitis E virus by using monoclonal antibodies. Journal of Virological Methods 2011, 171:374-380.
5. Greiner M. Two-graph receiver operating characteristic (TG-ROC): a Microsoft-EXCEL template for the selection of cut-off values in diagnostic tests. Journal of Immunological Methods 1995, 185:145-146.
6. Greiner M., Sohr D., Göbel P. A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnosis tests. Journal of Immunological Methods 1995, 185:123-132.
7. Haqshenas G., Shivaprasad H.L., Woolcock P.R., Read D.H., Meng X.J. Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States. Journal of General Virology 2001, 82:2449-2462.
8. Huang F.F., Haqshenas G., Shivaprasad H.L., Guenette P.R., Woolcock P.R., Larsen C.T., Pierson F.W., Elvinger F., Toth T.E., Meng X.J. Heterogeneity and seroprevalence of a newly identified avian hepatitis E virus from chickens in the United States. Journal of Clinical Microbiology 2002, 40:4197-4202.
9. Huang F.F., Sun Z.F., Emerson S.U., Purcell R.H., Shivaprasad H.L., Pierson F.W., Toth T.E., Meng X.J. Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV. Journal of General Virology 2004, 85:1609-1618.
10. Marek A., Bilic I., Prokofieva I., Hess M. Phylogenetic analysis avian hepatitis E virus samples from European and Australian chicken flocks supports the existence of a different genus within the Hepeviridae comprising at least three different genotypes. Veterinary Microbiology 2010, 145:54-61.
11. Payne C.J., Ellis T.M., Plant S.L., Gregory A.R., Wilcox G.E. Sequence data suggests big liver and spleen disease virus (BLSV) is genetically related to hepatitis E virus. Veterinary Microbiology 1999, 68:119-125.
12. Peralta B., Biarnés M., Ordóñez G., Porta R., Martín M., Mateu E., Pina S., Meng X.J. Evidence of widespread infection of avian hepatitis E virus (avian HEV) in chickens from Spain. Veterinary Microbiology 2009, 137:31-36.
13. Ritchie S.J., Riddell C. British Columbia. 'Hepatitis-splenomegaly' syndrome in commercial egg laying hens. Canadian Veterinary Journal 1991, 32:500-501.
14. Sun Z.F., Larsen C.T., Dunlop A., Huang F.F., Pierson F.W., Toth T.E., Meng X.J. Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis-splenomegaly syndrome in different geographical regions of the United States. Journal of General Virology 2004, 85:693-700.
15. Zhao Q., Zhou E.M., Dong S.W., Qiu H.K., Zhang L., Hu S.B., Zhao F.F., Jiang S.J., Sun Y.N. Analysis of avian hepatitis E virus from chickens, China. Emerging Infectious Diseases 2010, 16:1469-1472.