Immunofluorescence with dual microwave retrieval of paraffin-embedded sections in the assessment of human renal biopsy specimens

American Journal of Clinical Pathology

Volumn 139, Issue 1, 2013, Pages 71-78

  Shi, Suozhu ^a   Cheng, Qingli ^a   Zhang, Ping ^b   Wang, Nan ^a   Zheng, Ying ^a   Bai, Xue Yuan ^a   Chen, Xiangmei ^a  

^a CHINESE PLA GENERAL HOSPITAL   (China)

^b WANGJING HOSPITAL   (China)

Author keywords

Frozen tissue section; Immunofluorescence staining; Microwave retrieval; Paraffin embedded section; Renal biopsy specimen

Indexed keywords

ANTIGEN; COMPLEMENT COMPONENT C1Q; COMPLEMENT COMPONENT C3; FORMALDEHYDE; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN KAPPA CHAIN; IMMUNOGLOBULIN M; PARAFFIN;

ACUTE POSTINFECTIOUS GLOMERULONEPHRITIS; ARTICLE; BASEMENT MEMBRANE NEPHROPATHY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; FROZEN SECTION; GLOMERULONEPHRITIS; GLOMERULUS; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOGLOBULIN A NEPHROPATHY; INTERMETHOD COMPARISON; KIDNEY AMYLOIDOSIS; KIDNEY BIOPSY; LUPUS ERYTHEMATOSUS NEPHRITIS; MAJOR CLINICAL STUDY; MEMBRANOUS GLOMERULONEPHRITIS; MICROWAVE RADIATION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; TISSUE DISTRIBUTION;

BIOLOGICAL MARKERS; BIOPSY; FLUORESCENT ANTIBODY TECHNIQUE; FROZEN SECTIONS; HUMANS; IMMUNOGLOBULINS; KIDNEY; KIDNEY DISEASES; KIDNEY GLOMERULUS; MICROWAVES; PARAFFIN EMBEDDING; PREDICTIVE VALUE OF TESTS;

EID: 84871792007     PISSN: 00029173     EISSN: 19437722     Source Type: Journal    
DOI: 10.1309/AJCPRZG8EXN7BAID     Document Type: Article

References (23)

1. Molne J, Breimer ME, Svalander CT. Immunoperoxidase versus immunofluorescence in the assessment of human renal biopsies. Am J Kidney Dis. 2005;45:674-683. (Pubitemid 40467511)
2. Choi YJ, Reiner L. Immunofluorescence of renal lesions in paraffin-embedded and fresh-frozen sections. Am J Clin Pathol. 1980;73:116-119. (Pubitemid 10180408)
3. Fogazzi GB, Bajetta M, Banfi G, et al. Comparison of immunofluorescent findings in kidney after snap-freezing and formalin fixation. Pathol Res Pract. 1989;185:225-230. (Pubitemid 19224388)
4. Nasr SH, Galgano SJ, Markowitz GS, et al. Immunofluorescence on pronase-digested paraffin sections: a valuable salvage technique for renal biopsies. Kidney Int. 2006;70:2148-2151. (Pubitemid 44871379)
5. Qualman SJ, Keren DF. Immunofluorescence of deparaffinized, trypsin-treated renal tissues: preservation of antigens as an adjunct to diagnosis of disease. Lab Invest. 1979;41:483-489. (Pubitemid 10168514)
6. Morgan JM, Navabi H, Schmid KW, et al. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J Pathol. 1994;174:301-307.
7. Shi SR, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry: past, present, and future. J Histochem Cytochem. 1997;45:327-343. (Pubitemid 27120589)
8. Shi SR, Gu J, Turrens F. Development of the antigen retrieval technique: philosophy and theoretical base. In: Shi SR, Gu J, Taylor CR, eds. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. Natick, MA: Eaton; 2000:17-39.
9. Shi SR, Key ME, Kalar KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave ovenheating of tissue sections. J Histochem Cytochem. 1991;39:741-745.
10. Martins AR, Zanella CA, Zucchi FC, et al. Immunolocalization of nitric oxide synthase isoforms in human archival and rat tissues, and cultured cells. J Neurosci Methods. 2011;198:16-22.
11. Chetty R, Serra S, Gauchotte G, et al. Sclerosing nodular lesions of the gastrointestinal tract containing large numbers of IgG4 plasma cells. Pathology. 2011;43:31-35.
12. Ferris AM, Giberson RT, Sanders MA, et al. Advanced laboratory techniques for sample processing and immunolabeling using microwave radiation. J Neurosci Methods. 2009;182:157-164.
13. Saed GM. Immunohistochemical staining of cyclooxygenases with monoclonal antibodies. Methods Mol Biol. 2008;477:219-228.
14. Gao C, Wang AY, Han YJ. Microwave antigen retrieval blocks endogenous peroxidase activity in immunohistochemistry. Appl Immunohistochem Mol Morphol. 2008;16:393-399.
15. Liesegang A, Singer K, Boos A. Vitamin D receptor amounts across different segments of the gastrointestinal tract in Brown Swiss and Holstein Frisean cows of different age. J Anim Physiol Anim Nutr (Berl). 2008;92:316-323. (Pubitemid 351670174)
16. Leong AS, Haffajee Z. Citraconic anhydride: a new antigen retrieval solution. Pathology. 2010;42:77-81.
17. Lai FM, Lai KN, Chan KW, et al. Pattern of glomerulonephritis in Hong Kong. Pathology. 1987;19:247-252. (Pubitemid 17132301)
18. Chiu KY, Chan KW. Rapid immunofluorescence staining of human renal biopsy specimens using microwave irradiation. J Clin Pathol. 1987;40:689-692. (Pubitemid 17092857)
19. Leong AS, Milios J. Accelerated immunohistochemical staining by microwaves. J Pathol. 1990;161:327-334.
20. Bánkfalvi Á, Piffkó J, Öfner D, et al. Significance of wet autoclave pretreatment in immunohistochemistry. Pathol Oncol Res. 1996;2:71-77.
21. Kumada T, Tsuneyama K, Hatta H, et al. Improved 1-H rapid immunostaining method using intermittent microwave irradiation: practicability based on 5 years application in Toyama Medical and Pharmaceutical University hospital. Mod Pathol. 2004;17:1141-1149. (Pubitemid 39128241)
22. Rhodes A, Sarson J, Assam EE, et al. The reliability of rabbit monoclonal antibodies in the immunohistochemical assessment of estrogen receptors, progesterone receptors, and HER2 in human breast carcinomas. Am J Clin Pathol. 2010;134:621-632.
23. Chen X. How to evaluate the pathological diagnosis report of renal biopsy. Presented at 2011 Annual Conference of Chinese Association of Integrative Nephrology and 2011 International Congress of Integrative Nephrology; August 12-16, 2011; Beijing, China. PL-14, pp 34-36.