메뉴 건너뛰기




Volumn 7, Issue 5, 2012, Pages 921-934

A single vesicle-vesicle fusion assay for in vitro studies of snares and accessory proteins

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; SNARE PROTEIN;

EID: 84871527218     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2012.020     Document Type: Article
Times cited : (98)

References (34)
  • 4
    • 3543096759 scopus 로고    scopus 로고
    • Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4-angstrom resolution
    • Sutton, R.B., Fasshauer, D., Jahn, R., Brunger, A.T., Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4-angstrom resolution. Nature 395, 347-353 (1998).
    • (1998) Nature , vol.395 , pp. 347-353
    • Sutton, R.B.1    Fasshauer, D.2    Jahn, R.3    Brunger, A.T.4
  • 6
    • 67650759736 scopus 로고    scopus 로고
    • Single-molecule studies of the neuronal SNARE fusion machinery. Annu
    • Brunger, A.T., Weninger, K., Bowen, M., Chu, S., Single-molecule studies of the neuronal SNARE fusion machinery. Annu. Rev. Biochem. 78, 903-928 (2009).
    • (2009) Rev. Biochem , vol.78 , pp. 903-928
    • Brunger, A.T.1    Weninger, K.2    Bowen, M.3    Chu, S.4
  • 7
    • 0032549708 scopus 로고    scopus 로고
    • SNAREpins: Minimal machinery for membrane fusion
    • Weber, T. et al. SNAREpins: minimal machinery for membrane fusion. Cell 92, 759-772 (1998).
    • (1998) Cell , vol.92 , pp. 759-772
    • Weber, T.1
  • 8
    • 33646007069 scopus 로고    scopus 로고
    • SNARE-mediated lipid mixing depends on the physical state of the vesicles
    • Chen, X.C. et al. SNARE-mediated lipid mixing depends on the physical state of the vesicles. Biophys. J. 90, 2062-2074 (2006).
    • (2006) Biophys. J , vol.90 , pp. 2062-2074
    • Chen, X.C.1
  • 10
    • 2442569963 scopus 로고    scopus 로고
    • Imaging single membrane fusion events mediated by SNARE proteins
    • Fix, M. et al. Imaging single membrane fusion events mediated by SNARE proteins. Proc. Natl. Acad. Sci. USA 101, 7311-7316 (2004).
    • (2004) Proc. Natl. Acad. Sci. USA , vol.101 , pp. 7311-7316
    • Fix, M.1
  • 13
    • 78650137964 scopus 로고    scopus 로고
    • A single-vesicle content mixing assay for SNARE-mediated membrane fusion
    • Artn 54
    • Diao, J.J. et al. A single-vesicle content mixing assay for SNARE-mediated membrane fusion. Nat. Commun. 1, Artn 54 doi:10.1038/Ncomms1054 (2010).
    • (2010) Nat. Commun , vol.1
    • Diao, J.J.1
  • 14
    • 59049093107 scopus 로고    scopus 로고
    • Effects of linker sequences on vesicle fusion mediated by lipid-anchored DNA oligonucleotides
    • Chan, Y.H.M., van Lengerich, B., Boxer, S.G., Effects of linker sequences on vesicle fusion mediated by lipid-anchored DNA oligonucleotides. Proc. Natl. Acad. Sci. USA 106, 979-984 (2009).
    • (2009) Proc. Natl. Acad. Sci. USA , vol.106 , pp. 979-984
    • Chan, Y.H.M.1    Van Lengerich, B.2    Boxer, S.G.3
  • 15
    • 0018876557 scopus 로고
    • Fusion of phospholipid-vesicles with planar phospholipid-bilayer membranes.1.Discharge of vesicular contents across the planar membrane
    • Zimmerberg, J., Cohen, F.S., Finkelstein, A., Fusion of phospholipid-vesicles with planar phospholipid-bilayer membranes.1. Discharge of vesicular contents across the planar membrane. J. General Phys. 75, 241-250 (1980).
    • (1980) J. General Phys , vol.75 , pp. 241-250
    • Zimmerberg, J.1    Cohen, F.S.2    Finkelstein, A.3
  • 16
    • 0036932526 scopus 로고    scopus 로고
    • Early steps of supported bilayer formation probed by single vesicle fluorescence assays
    • Johnson, J.M., Ha T., Chu, S., Boxer, S.G., Early steps of supported bilayer formation probed by single vesicle fluorescence assays. Biophys. J. 83, 3371-3379 (2002).
    • (2002) Biophys. J , vol.83 , pp. 3371-3379
    • Johnson, J.M.1    Ha, T.2    Chu, S.3    Boxer, S.G.4
  • 18
    • 0033607182 scopus 로고    scopus 로고
    • Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs
    • Nickel, W. et al. Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs. Proc. Natl. Acad. Sci. USA 96, 12571-12576 (1999).
    • (1999) Proc. Natl. Acad. Sci. USA , vol.96 , pp. 12571-12576
    • Nickel, W.1
  • 19
    • 0035819204 scopus 로고    scopus 로고
    • Immobilization in surface-tethered lipid vesicles as a new tool for single biomolecule spectroscopy
    • Boukobza, E., Sonnenfeld, A., Haran, G., Immobilization in surface-tethered lipid vesicles as a new tool for single biomolecule spectroscopy. J. Phys. Chem. B 105, 12165-12170 (2001).
    • (2001) J. Phys. Chem. B , vol.105 , pp. 12165-12170
    • Boukobza, E.1    Sonnenfeld, A.2    Haran, G.3
  • 20
    • 46049088692 scopus 로고    scopus 로고
    • The SNARE complex from yeast is partially unstructured on the membrane
    • Su, Z.L., Ishitsuka, Y., Ha T., Shin, Y.K. The SNARE complex from yeast is partially unstructured on the membrane. Structure 16, 1138-1146 (2008).
    • (2008) Structure , vol.16 , pp. 1138-1146
    • Su, Z.L.1    Ishitsuka, Y.2    Ha, T.3    Shin, Y.K.4
  • 21
    • 46449139821 scopus 로고    scopus 로고
    • Complexin and Ca2+stimulate SNARE-mediated membrane fusion
    • Yoon, T.Y. et al. Complexin and Ca2+stimulate SNARE-mediated membrane fusion. Nat. Struct. Mol. Biol. 15, 707-713 (2008).
    • (2008) Nat. Struct. Mol. Biol , vol.15 , pp. 707-713
    • Yoon, T.Y.1
  • 22
    • 67650021208 scopus 로고    scopus 로고
    • C2AB: A molecular glue for lipid vesicles with a negatively charged surface
    • Diao, J.J., Yoon, T.Y., Su, Z.L., Shin, Y.K., Ha T., C2AB: a molecular glue for lipid vesicles with a negatively charged surface. Langmuir 25, 7177-7180 (2009).
    • (2009) Langmuir , vol.25 , pp. 7177-7180
    • Diao, J.J.1    Yoon, T.Y.2    Su, Z.L.3    Shin, Y.K.4    Ha, T.5
  • 23
    • 77951729758 scopus 로고    scopus 로고
    • Single-vesicle fusion assay reveals Munc18-1 binding to the SNARE core is sufficient for stimulating membrane fusion. ACS
    • Diao, J.J. et al. Single-vesicle fusion assay reveals Munc18-1 binding to the SNARE core is sufficient for stimulating membrane fusion. ACS Chem. Neurosci. 1, 168-174 (2010).
    • (2010) Chem. Neurosci , vol.1 , pp. 168-174
    • Diao, J.J.1
  • 24
    • 77952231768 scopus 로고    scopus 로고
    • Dynamic Ca2+-dependent stimulation of vesicle fusion by membrane-anchored synaptotagmin 1
    • Lee, H.K. et al. Dynamic Ca2+-dependent stimulation of vesicle fusion by membrane-anchored synaptotagmin 1. Science 328, 760-763 (2010).
    • (2010) Science , vol.328 , pp. 760-763
    • Lee, H.K.1
  • 26
    • 70450235266 scopus 로고    scopus 로고
    • Single vesicle millisecond fusion kinetics reveals number of SNARE complexes optimal for fast SNARE-mediated membrane fusion
    • Domanska, M.K., Kiessling, V., Stein, A., Fasshauer, D., Tamm, L.K., Single vesicle millisecond fusion kinetics reveals number of SNARE complexes optimal for fast SNARE-mediated membrane fusion. J. Biol. Chem. 284, 32158-32166 (2009).
    • (2009) J. Biol. Chem , vol.284 , pp. 32158-32166
    • Domanska, M.K.1    Kiessling, V.2    Stein, A.3    Fasshauer, D.4    Tamm, L.K.5
  • 28
    • 77649239540 scopus 로고    scopus 로고
    • A fast, single-vesicle fusion assay mimics physiological SNARE requirements
    • Karatekin, E. et al. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proc. Natl. Acad. Sci. USA 107, 3517-3521 (2010).
    • (2010) Proc. Natl. Acad. Sci. USA , vol.107 , pp. 3517-3521
    • Karatekin, E.1
  • 29
    • 79959729394 scopus 로고    scopus 로고
    • Docking, not fusion, as the rate-limiting step in a SNARE-driven vesicle fusion assay
    • Smith, E.A., Weisshaar, J.C., Docking, not fusion, as the rate-limiting step in a SNARE-driven vesicle fusion assay. Biophys. J. 100, 2141-2150 (2011).
    • (2011) Biophys. J , vol.100 , pp. 2141-2150
    • Smith, E.A.1    Weisshaar, J.C.2
  • 30
    • 84870037460 scopus 로고    scopus 로고
    • Fusion of single proteoliposomes with planar, supported bilayers in microfluidic flow cells. Nat
    • Karatekin, E., Rothman, J.E., Fusion of single proteoliposomes with planar, supported bilayers in microfluidic flow cells. Nat. Protoc. 7, 903-920 (2012).
    • (2012) Protoc , vol.7 , pp. 903-920
    • Karatekin, E.1    Rothman, J.E.2
  • 31
    • 50649121477 scopus 로고    scopus 로고
    • Advances in single-molecule fluorescence methods for molecular biology. Annu
    • Joo, C., Balci, H., Ishitsuka, Y., Buranachai, C., Ha T., Advances in single-molecule fluorescence methods for molecular biology. Annu. Rev. Biochem. 77, 51-76 (2008).
    • (2008) Rev. Biochem , vol.77 , pp. 51-76
    • Joo, C.1    Balci, H.2    Ishitsuka, Y.3    Buranachai, C.4    Ha, T.5
  • 32
    • 44449134820 scopus 로고    scopus 로고
    • A practical guide to single-molecule FRET. Nat
    • Roy, R., Hohng, S., Ha T., A practical guide to single-molecule FRET. Nat. Methods 5, 507-516 (2008).
    • (2008) Methods , vol.5 , pp. 507-516
    • Roy, R.1    Hohng, S.2    Ha, T.3
  • 33
    • 44449103347 scopus 로고    scopus 로고
    • Single Molecule Techniques
    • Cold Spring Harbor Laboratory Press
    • Selvin, P.R.H., Ha T., Single Molecule Techniques: A Laboratory Manual(Cold Spring Harbor Laboratory Press, 2008).
    • (2008) A Laboratory Manual
    • Selvin, P.R.H.1    Ha, T.2
  • 34
    • 0029670262 scopus 로고    scopus 로고
    • Molecular beacons: Probes that fluoresce upon hybridization. Nat
    • Tyagi, S., Kramer, F.R., Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14, 303-308 (1996).
    • (1996) Biotechnol , vol.14 , pp. 303-308
    • Tyagi, S.1    Kramer, F.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.