Usefulness of capillary electrophoresis-based multiplex PCR assay for species-specific identification of Candida spp.

Journal of Microbiological Methods

Volumn 92, Issue 2, 2013, Pages 150-152

  Mallus, F ^a   Martis, S ^a   Serra, C ^a   Loi, G ^a   Camboni, T ^a   Manzin, A ^a  

^a UNIVERSITY OF CAGLIARI   (Italy)

Author keywords

Candida spp.; Capillary electrophoresis; Multiplex PCR; Species specific identification

Indexed keywords

ARTICLE; CANDIDA; CAPILLARY ELECTROPHORESIS; CLINICAL LABORATORY; CONTROLLED STUDY; FUNGAL STRAIN; INTERMETHOD COMPARISON; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PERFORMANCE; PRIORITY JOURNAL; RELIABILITY; SPECIES IDENTIFICATION;

CANDIDA; ELECTROPHORESIS, CAPILLARY; HUMANS; MICROBIOLOGICAL TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOLOGY; TIME FACTORS;

CANDIDA;

EID: 84871488898     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.11.022     Document Type: Article

References (15)

1. Ahmad S., Khan Z. Invasive candidiasis: a review of nonculture-based laboratory diagnostic methods. Indian J. Med. Microbiol. 2012, 30:264-269.
2. Avni T., Leibovivi L., Paul M. PCR diagnosis of invasive candidiasis: systemic review and meta-analysis. J. Clin. Microbiol. 2011, 49:665-670.
3. Chang H.C., Leaw S.N., Huang A.H., Wu T.L., Chang T.C. Rapid identification of yeasts in positive blood cultures by a multiplex PCR method. J. Clin. Microbiol. 2001, 39:3466-3471.
4. Ferroni A., Suarez S., Beretti J.L., Dauphin B., Bille E., Meyer J., Bougnoux M.E., Alanio A., Berche P., Nassif X. Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 2010, 48:1542-1548.
5. Khlif M., Mary C., Sellami H., Sellami A., Dumon H., Ayadi A., Ranque S. Evaluation of nested and real-time PCR assays in the diagnosis of candidemia. Clin. Microbiol. Infect. 2009, 15:656-661.
6. Lee M.K., Kim H.R., Lee Y.J. Identification of Candida species by multiplex polymerase chain reaction. Korean J. Clin. Microbiol. 2006, 9:119-124.
7. Liguori G., Gallé F., Lucariello A., Di Onofrio V., Albano L., Mazzarella G., D'Amora M., Rossano F. Comparison between multiplex PCR and phenotypic systems for Candida spp. identification. New Microbiol. 2010, 33:63-67.
8. Logotheti M., Kotsovili-Tseleni A., Arsenis G., Legakis N.I. Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus. J. Microbiol. Meth. 2009, 76:209-211.
9. Luo G., Mitchell T.G. Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR. J. Clin. Microbiol. 2002, 40:2860-2865.
10. Merz W.G., Roberts G.D. Algorithms for detection and identification of fungi. Manual of Clinical Microbiology 2003, 1668-1685. American society for microbiology, Washington DC. P.R. Murray, E.J. Baron, J.H. Jorgensen, M.A. Pfaller, R.H. Yolken (Eds.).
11. Mikulska M., Calandra T., Sanguinetti M., Poulain D., Viscoli C., Third European Conference on Infections in Leukemia Group The use of mannan antigen and anti-mannan antibodies in the diagnosis of invasive candidiasis: recommendations from the Third European Conference on Infections in Leukemia. Crit. Care 2010, 14:R222.
12. Peres-Bota D., Rodriguez-Villalobos H., Dimopoulos G., Melot C., Vincent J.L. Potential risk factors for infection with Candida spp. in critically ill patients. Clin. Microbiol. Infect. 2004, 10:550-555.
13. Pfaller M.A., Diekema D.J. Epidemiology of invasive candidiasis: a persistent public health problem. Clin. Microbiol. Rev. 2007, 20:133-163.
14. Romeo O., Scordino F., Pernice I., Lo Passo C., Criseo G. A multiplex PCR protocol for rapid identification of Candida glabrata and its phylogenetically related species Candida nivariensis and Candida bracarensis. J. Microbiol. Meth. 2009, 79:117-120.
15. Stevenson J.B., Hymas W.C., Hillyard D.R. A novel capillary electrophoresis-based multiplex PCR assay for detection of respiratory pathogens. Ann. Clin. Lab. Sci. 2011, 41:33-38.