Biological activity analysis of native and recombinant treptokinase using clot lysis and chromogenic substrate assay

Iranian Journal of Pharmaceutical Research

Volumn 11, Issue 4, 2012, Pages 1087-1093

  Mahboubi, Arash ^a   Sadjady, Seyyed Kazem ^b   Abadi, Mohammad Mirzaei Saleh ^c   Azadi, Saeed ^c   Solaimanian, Roya ^c  

^a SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b ISLAMIC AZAD UNIVERSITY   (Iran)

^c Daroupakhsh Pharmaceutical Company   (Iran)

Author keywords

Biological activity; Chromogenic substrate; Clot lysis; Native streptokinase; Recombinant streptokinase

Indexed keywords

CHROMOGENIC SUBSTRATE; HEBERKINASA; METHIONINE; PLASMINOGEN; RECOMBINANT STREPTOKINASE; STREPTOKINASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOLOGICAL ACTIVITY; BLOOD CLOT LYSIS; CONTROLLED STUDY; DOSE RESPONSE; ENZYME ACTIVATION; HUMAN; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

EID: 84871356795     PISSN: 17350328     EISSN: 17266890     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Hermentin P, Cuesta-Linker T, Weisse J, Schmidt KH, Knorst M, Scheld M and Thimme M. Comparative analysis of the activity and content of different streptokinase preparations. Eur. Heart J. (2005) 26: 933-940.
2. th List, March 2011. Available at: http://whqlibdoc.who.int/hq/2011/a95053_eng.pdf.
3. Thelwell C. Fibrinolysis standards: A review of the current status. Biologicals (2010) 38: 437-448
4. Longstaff C, Thelwell C and Whitton C. The poor quality of streptokinase products in use in developing countries. J. Thromb. Haemost. (2005) 3: 1092-1093.
5. Banerjee A, Chisti Y and Banerjee UC. Streptokinase a clinically useful thrombolytic agent. Biotechnol. Adv. (2004) 22: 287-307
6. Couto LT, Donato JL and de Nucci G. Analysis of fve streptokinase formulations using the euglobulin lysis test and the plasminogen activation assay. Braz. J. Med. Biol. Res. (2004) 37: 1889-1894.
7. Mundada LV, ProrokM, DeFordME, FigueraM, Castellino FJ and Fay WP. Structure-function analysis of the streptokinase amino terminus (residues 1-59). J. Biol. Chem. (2003) 278: 24421-24427.
8. Marsh NA and Gaffney PJ. The rapid fibrin plate-a method for plasminogen activator assay. Thromb. Hemost. (1977) 38: 545- 551.
9. rdInternational Standard for Streptokinase. J. Thromb. Haemost. (2004) 2: 1411-1415.
10. Hernández L and Marrero M A. Streptokinase: about of a thrombolytic patented in Cuba. Biotecnologia Aplicada (2005) 22:191-198.
11. Hernández L, Martinez Y, Quintana M, Besada V and Martinez E. Heberkinasa: recombinant streptokinase. Eur. Heart J. (2005) 26:1691.
12. Salarifar M, Sadeghian S, Abbasi A, Davoodi G, Amirzadegan A, Hosseini SK, Paydari N, Biria A and Moemeni P. Efficacy of two streptokinase formulation in acute myocardial infarction: A double-blind randomized clinical trial. J. Tehran Univ. Heart Center (2009) 1: 29-34.
13. Gladysheva IP, Turner RB, Sazonova IY, Liu L and Reed GL. Coevolutionary patterns in plasminogen activation. Proc. Natl. Acad. Sci. USA (2003) 100: 9168-9172.
14. Wang S, Reed GL and Hedstrom L. Zymogen activation in the streptokinase-plasminogen complex Ile1 is required for formation of a functional active site. Eur. J. Biochem. (2000) 267: 3994-4001.
15. Longstaff C, Whitton C, Stebbings R and Gray E. How do we assure the quality of biological medicines? Drug Discovery Today (2009) 14: 50-55.
16. Longstaff C. Standardization of fibrinolytic factors. Proceedings of the Fibrinolysis Congress. Oslo Kongressenter, Norway, 30 June 2006.
17. Sazonova IY, Mcnamee RA, Houng AK, King SM, Hedstrom L and Reed GL. Reprogrammed streptokinase develop fibrin-targeting and dissolve blood clots with more potency than tissue plasminogen activator. J. Thromb. Haemost. (2009)7: 1321-1328.
18. Monograph. Streptokinse. British Pharmacopeia. (2003): 0356.
19. Monograph. Streptokinse bulk solution. British Pharmacopeia. (2006): 0356.
20. Jackson CM, White GC, Barrowcliffe T, Esnoul MP, Jespersen J, Kluft C, Lenahan J. A reference system approach to future standardization of laboratory tests for hemostasis. Thromb. Haemost. (2002) 87: 165-6.